REDOX REACTION BASED SPECTROPHOTOMETRIC ASSAY OF SOME DRUGS IN PHARMACEUTICALS
Keywords:
Pyridoxine Hydrochloride, Dobutamine Hydrochloride, Linezolid Form-1, Folin Ciocalteu reagent, Spectrophotometry, PharmaceuticalsAbstract
Objectives: Pyridoxine hydrochloride (PRC) is used in the treatment of side roblastic anemia's and is also used in a variety of disorders including the treatment of depression. Dobutamine Hydrochloride (DOB) is used in the case of congestive heart failure to increase cardiac output and is also commonly used in the hospital setting as a pharmacologic stress testing agent to identify coronary artery disease. Linezolid (LZD) is a synthetic antibiotic used for the treatment of serious infections caused by gram-positive bacteria that are resistant to several other antibiotics. The main objective of our method is to develop a simple, accurate and sensitive spectrophotometric method for the assay of the above mentioned drugs in both tablet and pharmaceutical dosage forms.
Methods: The method is based on the red ox reaction of drugs with Folin Ciocalteu (FC) reagent in sodium carbonate medium and the resulting blue colored chromogen is measured at 755 nm.
Results: Beer's law is obeyed in the concentration range of 2.5–30 µg/ml (PRC), 1–10 µg/ml (DOB) and 2.5–70 µg/ml (LZD) respectively, with the corresponding molar absorptivity values of 7.145 X 103, 3.2080 X 104 and 6.299 X 103 l mol-1 cm-1. The method is validated for accuracy, precision, LOD, LOQ, robustness and ruggedness as per the current ICH guidelines.
Conclusion: The validated method is successfully applied to quantify PRC, DOB and LZD in their commercial formulations with satisfactory results; hence the method is suitable for the determination of drugs in bulk and pharmaceuticals.
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References
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