CHEMICAL CHARACTERISATION OF ALPINIA GALANGA (L.) WILLD BY GC–MS, XRD, FTIR AND UV-VIS SPECTROSCOPIC METHODS

Abstract

Objective: Present study was aimed to produce the chemical profile of Alpinia galanga (L.) Willd by various analytical methods.

Methods: The sample extracted with methanol were screened for their volatile organic constituents using a Shimadzu QP-2010 PLUS gas chromatograph coupled to a mass spectrometer (GC-MS) instrument (Shimadzu Corporation, Japan). Then the sample was analyzed for the identification of unknown crystalline materials and determination of crystal structure using a PANalytical X'PERT PRO X-ray diffractometer. Finally the extracts were examined under visible and UV light for the proximate analysis. FTIR method performed on IR AFFINITY-1 Spectrophotometer and UV-1700 Spectrophotometer detects characteristic peaks in the visible range.

Results: Forty significant compounds were identified in A. galanga by gas chromatography-mass spectrometry method. XRD pattern gave three prominent diffraction peaks at 25 position (15.39 ^o, 17.42^o and 31.32^o) results in d-spacing value 5.75, 5.08 and 2.85 Ã…; confirms the presence of Cu, Si and Pb elements. FTIR analysis confirmed the presence of alkanes, amides, carboxylic acids, epoxides, alcohols, aliphatic amines, aromatics and phenol compounds. UV Vis spectrum profile showed the peaks at 358, 268 and 224 nm with the absorption of 0.590, 1.199 and 2.752.

Conclusion: The information regarding the characterization and quantification may be useful in assessing the genotoxicity of the plant material and can be recommended for implementation in the official pharmacopeias.