HISTOMORPHOMETRIC ANALYSIS IN KIDNEY TISSUE OF MICE TREATED WITH INDIGOFERA SUFFRUTICOSA MILL

Authors

  • Marllon Alex Nascimento Santana Department of Embryology and Histology/Universidade Federal de Pernambuco, Recife-PE, Brazil
  • Ivanise Brito Da Silva Department of Embryology and Histology/Universidade Federal de Pernambuco, Recife-PE
  • JESSICA ANDREIA PEREIRA BARBOSA Department of Antibiotics/Universidade Federal de Pernambuco, Recife-PE, Brazil
  • Izabela Rangel Lima Department of Embryology and Histology/Universidade Federal de Pernambuco, Recife-PE
  • Jeymesson Raphael Cardoso Vieira Department of Embryology and Histology/Universidade Federal de Pernambuco, Recife-PE
  • Roberta Maria Pereira Leite Lima Department of Embryology and Histology/Universidade Federal de Pernambuco, Recife-PE, Brazil
  • SONIA PEREIRA LEITE Department of Embryology and Histology/Universidade Federal de Pernambuco, Recife-PE, Brazil

Keywords:

Histomorphometry, Indigofera suffruticosa, Kidney, Sarcoma 180

Abstract

Objective: To investigate by histomorphometry the effect of aqueous extract of Indigofera suffruticosa leaves on proximal convoluted tubules and renal corpuscles of mice with sarcoma 180.

Methods: Male swiss albino mice (Mus musculus) with 60 days of age were divided into 3 groups of 6 animals: Group 1-sarcoma 180 treated with aqueous extract (50 mg/kg, intraperitoneally (i. p.)), Group 2-sarcoma 180 treated with saline solution (15 ml/kg, i. p.) and Group 3–without sarcoma 180 treated with saline (15 ml/kg, i. p.). After 48 hours of tumor implantation, the treatment was initiated during 7 days. Histomorphometric analysis of kidney tissue was performed using IMAGEJ software.

Results: Antitumoral treatment with aqueous extract of I. suffruticosa leaves at a dose of 50 mg/kg has not caused mice renal tissue histological changes.

Conclusion: After treatment with aqueous extract of leaves of I. suffruticosa, renal architecture was preserved, suggesting its safety use as an antitumor agent.

 

Downloads

Download data is not yet available.

Author Biographies

Marllon Alex Nascimento Santana, Department of Embryology and Histology/Universidade Federal de Pernambuco, Recife-PE, Brazil

Department of Histology and Embriology

Ivanise Brito Da Silva, Department of Embryology and Histology/Universidade Federal de Pernambuco, Recife-PE

Department of Histology and Embriology

JESSICA ANDREIA PEREIRA BARBOSA, Department of Antibiotics/Universidade Federal de Pernambuco, Recife-PE, Brazil

Department of Antibiotics

Izabela Rangel Lima, Department of Embryology and Histology/Universidade Federal de Pernambuco, Recife-PE

Department of Histology and Embriology

Jeymesson Raphael Cardoso Vieira, Department of Embryology and Histology/Universidade Federal de Pernambuco, Recife-PE

Department of Histology and Embriology

Roberta Maria Pereira Leite Lima, Department of Embryology and Histology/Universidade Federal de Pernambuco, Recife-PE, Brazil

Department of Histology and Embriology

SONIA PEREIRA LEITE, Department of Embryology and Histology/Universidade Federal de Pernambuco, Recife-PE, Brazil

Department of Histology and Embriology

References

Elizabetsky E. Resenha: fitoterapia com base científica. Ciência Hoj, 2002;31:78-9.

López, CAA. Considerações gerais sobre plantas medicinais. Ambiente: Gestão e Desenvolvimento 2006;1:19-27.

Calvo TR, Cardoso CR, Silva MAC, Santos LC, Colus IM, Vilega W, et al. Mutagenic activity of Indigofera truxillensis and I. suffruticosa aerial parts. J Evidence-Based Complementary Altern Med 2009;20:1-8.

Leite SP, Silva LLS, Catanho MTJA, Lima EO, Lima VLM. Anti-inflammatory activity of Indigofera suffruticosa extract. Revbrasa 2003;7:47–52.

Leite SP, Silva EC, Cassilhas APP, Torquanto GN, Silva LLS, Medeiros PL, et al. Atividade antiparasitária de frações aquosas de Lonchocarpus araripenses (Papilionaceae) e Indigofera suffruticosa (Fabaceae). 55ª Reunião Anual da SBPC–Resumo 4694 C 13.4. Recife; 2003.

Leite SP, Medeiros PL, Silva EC, Maia MBS, Lima VLM, Saul DE. Embryotoxicity in vitro with extract of Indigofera suffruticosa leaves. Reprod Toxicol 2004;18:701-5.

Leite SP, Vieira JRC, Leite RMP. Antimicrobial activity of Indigofera suffruticosa. J Evidence Based Complementary Altern Med 2006;3:261-5.

Vieira JRC, Souza IA, Nascimento SC, Leite SP. Antitumoractivity of indigofera suffruticosa. An Fac Med Univ Recife 2007;52:112-5.

Vieira JRC, Souza IA, Nascimento SC, Leite SP. Indigofera suffruticosa: an alternative anticancer therapy. J Evidence Based Complementary Altern Med 2007;4:355-9.

Vieira JRC. Investigação farmacognóstica e biológica de folhas de Indigofera suffruticosa Mill sobre Aedes aegypti. Tese (Doutorado em Ciências Farmacêuticas)–Departamento de Ciências Farmacêuticas. Recife: Universidade Federal de Pernambuco; 2011.

Golan DE, Tashjian AH, Armstrong EJ, Armstrong AW. Princípios de farmacologia-a base fisiopatológica da farmacoterapia. 3nd ed. Guanabara; 2014. p. 972.

Laib A, Newitt DC, Lu Y, Majundar S. New model-independent measures of trabecular bone structure applied to in vivo high-resolution MR images. Osteoporosis Int 2002;13:130-6.

Freemont T, Malluche HH. Utilization of bone histomorphometry in renal osteodystrophy: demonstration of a new approach using data from a prospective study of lanthanum carbonate. Clin Nephrol 2005;63:138-45.

Weinsten R. Clinical use of bone biopsy. In: Marcus R, Feldman D, Nelson D, Rosen C J. editors. Osteoporosis. 3nd ed. 2008;448-65.

Gartner LP, Hiatt JL. Atlas Colorido de Histologia. 4 nd ed. Guanabara Koogan, Rio de Janeiro; 2007. p. 431.

Kuppuswamy R, Govindara A, Velusamy G, Balasubramanian R, Balasundarm J, Sellamuthu M. Effect of dried fruits of solanum nigrum LINN against CCl4-induced hepatic damage in rats. Biol Pharm Bull 2003;26:1618-9.

Lima IR, Vieira JRC, Silva IB, Leite RMP, Maia MB, Leite SP. Indican from Añil (Indigofera suffruticosa Miller) as an herbal protective agent to the liver. Anal Quant Cytol Histol 2014;36:41-5.

Silva IB, Lima IR, Santana MAN, Leite RMP, Leite, SP. Indigofera suffruticosa Mill (Fabaceae): hepatic responses in mice bearing sarcoma 180. Int J Morphol 2014;32:1228-33.

Published

01-09-2015

How to Cite

Santana, M. A. N., I. B. D. Silva, J. A. P. BARBOSA, I. R. Lima, J. R. C. Vieira, R. M. P. Leite Lima, and S. P. LEITE. “HISTOMORPHOMETRIC ANALYSIS IN KIDNEY TISSUE OF MICE TREATED WITH INDIGOFERA SUFFRUTICOSA MILL”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 9, Sept. 2015, pp. 505-6, https://mail.innovareacademics.in/journals/index.php/ijpps/article/view/7004.

Issue

Section

Short Communication(s)

Most read articles by the same author(s)