ANTIDIABETIC AND ANTIOXIDANT ACTIVITY OF RESVERATROL AND VITAMIN-C COMBINATION ON STREPTOZOTOCIN INDUCED DIABETIC RATS
Keywords:
Streptozotocin, Diabetes, Synergistic activity, ResveratrolAbstract
Objective: Resveratrol and vitamin-C combination was evaluated for its antidiabetic and antioxidant activity in streptozotocin (STZ) induced diabetic rats.
Methods: The wistar rats were challenged with a single intra peritoneal injection of STZ (50 mg/kg). The rats were treated with graded oral dose of 10 mg/kg of resveratrol and 0.9 g/kg of Vitamin C for 21 days. The fasting blood glucose levels were monitored for all animals in 1,7,14 and 21 days of drug treatments by using glucometer. The liver homogenate was used for estimation of proteins, malondialdehyde (MDA), lipid hydroperoxide (LH), Super Oxide Dismutase (SOD), Catalase (CAT), Glutathione reductase (GSSH) and Reduced glutathione (GSH).
Results: Resveratrol with vitamin C combination showed significant (P<0.01) increase in body weight, total protein, and significant (P<0.01) decrease in fasting blood sugar level, MDA and LH, when compared to diabetic control. Resveratrol and vitamin C significantly (P<0.01) restored the levels of both enzymatic (SOD, CAT, GSSH) and non enzymatic antioxidant enzymes (GSH) which are almost similar to the control.
Conclusion: Resveratrol and vitamin C both is having antioxidant activity, but the combination showed synergistic antioxidant & antidiabetic activity. Thus, the present study provides a significant rationale for the management of diabetes.
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