EVALUATION OF IMMUNOMODULATORY AND ANTIOXIDANT ACTIVITIES OF POLYSACCHARIDES ISOLATED FROM CALLICARPA MACROPHYLLA VAHL.
Keywords:
Callicarpa macrophylla, Lymphocyte proliferation assay, DPPH assay, Total antioxidant assayAbstract
Objective: To evaluate the immunomodulatory and antioxidant activities of polysaccharides from Callicarpa macrophylla Vahl. leaves.
Methods: Three different fractions of polysaccharides (PC, PH, PA) were isolated. The percentage of sugar in the extracted samples was checked by phenol-sulphuric acid method. Lymphocyte proliferation assay was done to evaluate its immunomodulatory activity. 1, 1-Diphenyl-2-picrylhydrazyl (DPPH) assay and total antioxidant assay was done to evaluate its antioxidant property.
Results: The total polysaccharide content in PC, PH and PA was found to be 88%, 14% and 87% by the phenol-sulphuric acid method. All the three polysaccharides showed in vitro growth stimulatory effect on isolated normal lymphocytes. The proliferative index of PC, PH, PA was found to be 1.28±0.03, 1.71±0.04 and 1.12±0.01 at a concentration of 500 μg/ml indiÂcating immunomodulatory activity. PC, PH, PA showed a % inhibition of DPPH radical at 40.11±0.005 %, 29.18±0.01 % and 16.82±0.007 %. A dose-dependent activity was shown for the DPPH assay. Total antioxidant activity was found to be higher in PA. Both PC and PH showed almost equal antioxidant activity.
Conclusion: The results of the present study indicates that polysaccharides from Callicarpa macrophylla Vahl. boosts the immune system and help overcome the negative effects of oxidative stress, thus, contributing to the development of new drugs.
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References
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