A STUDY TO PREDICT ANTI-INFLAMMATORY ACTIVITY OF EUGENOL, MYRISTICIN, AND LIMONENE OF CINNAMOMUM SINTOC
Keywords:
Anti-inflammatory, Cinnamomum sintoc, Cyclooxygenase, Eugenol, Limonene, MyristicinAbstract
Objective: In this work we predicted anti-inflammatory activity of volatile oil of C. sintoc L.
Methods: Molecular docking was performed to predict the binding modes of eugenol, myristicin, and limonene chemical constituents of C. sintoc L. with COX enzymes, using Auto Dock 4.2. COX enzymes were obtained from Protein Data Bank (PDB); COX-1 (PDB code: 2AYL) and COX-2 (PDB code: 3PGH). Flurbiprofen and celecoxib were used as standards. Further assay was carried out on lipopolysaccharide (LPS)-induced fibroblast cells reacted with 800; 400; 200; 100; 50; 25 and 12.5 ul of C. sintoc L. bark essential oils. The absorbance of the product was measured using microplate reader at 450 nm. Acetosal was used as the standard drug.
Results: Eugenol and myristicin could be categorized as non-selective inhibitors of COX-2, while limonene is categorized as preferential COX-2 inhibitor. The essential oils of C. sintoc L. bark reduced PGE2 production on LPS-induced fibroblast cells. The inhibitory activity of C. sintoc L. was weaker than acetosal.
Conclusion: Bioactive compounds in essential oil of C. sintoc L. bark show inhibition on PGE2 production on LPS-induced human fibroblast cells, and could be categorized as COX inhibitors.
Â
Downloads
References
Sumiwi SA, Subarnas A, Supriyatna S, Abdasah M, Muchtaridi M. Analysis of chemical composition and its analgesic and anti-inflammatory activity of essential oil of sintoc bark (Cinnamomum sintoc Bl.) using in vivo methods. J Appl Pharm Sci 2015;5:58-65.
Sharma V, Rao LJM. An overview on chemical composition, Bioactivity and processing of leaves of Cinnamomum tamala. Crit Rev Food Sci Nutr 2012;54:433-48.
Leem JY, Wansu P. Anti-Inflammatory effect of myristicin on RAW 264.7 macrophages stimulated with polyinosinic-polycytidylic acid. Molecules 2011;16:7132-42.
Ozaky Y, Soedigdo S, Wattimena YR, Suganda AG. Anti-inflammatory effect of mace, aril of Myristica fragrans houtt and its active principles. Jpn J Pharmacol 1989;49:155-63.
Yoon, WJ, Lee NH, Hyun CG. Limonene supresses lipopolysaccharide-induced production of nitric oxide, prostaglandin E2 and Pro-Inflammatory cytokines in RAW 264.7 macrophages. J Oleo Sci 2010;59:415-21.
Rahman MU, Tahir M, Khan AQ, Khan R, Oday OH, Lateef A, et al. D-Limonene suppresses doxorubicin-induced oxidative stress and inflammation via repression of COX-2, iNOS, and NFkB in kidneys of wistar rats. Exp Biol Med 2014;239:465-76.
Levita J, Nawawi A, Mutholib A, Ibrahim S. Andrographolide Inhibits COX-2 Expression in human fibroblast cells due to its interaction with arginine and histidine in cyclooxygenase site. J Appl Sci 2010;10:1481-4.
Nunthanavanit P, Samee W. Molecular docking of natural product-derived compounds: estimation of selectivity on Cyclo-oxygenase-2. Thai Pharm Health Sci J 2011;6:79-85.