ANTI-PROLIFERATIVE AND APOPTOTIC ACTIVITIES OF SYZYGIUM SAMARANGENSE (WAX APPLE) FRUITS EXTRACT AGAINST HUMAN A549 LUNG CANCER CELL LINES
Keywords:
Syzygium samarangense, Anti-proliferative activity, MTT assay, DNA Fragmentation, Apoptotic activity, PI stainingAbstract
Objective: Medicinal plants have been in use from time immemorial and their utility has been expanding step by step in the present world. The intention of the present study was to check the anti-proliferative and apoptotic activity of Syzygium samarangense fruits methanolic extract against of A549 cell lines.
Methods: The methanol extract at different concentrations were tested against A549 human lung cancer cell lines for cell viability or cytotoxicity by MTT assay and the hallmark of the apoptosis was analyzed by DNA fragmentation method. The morphological changes resulted due to apoptosis were investigated by Propidium Iodide (PI) staining technique.
Results: The results showed that the tested extracts showed strong and decreased cell viability in a concentration-dependent manner. IC50 value represented that the anti-proliferative activity was found with a minimum concentration of 21.86μg/ml. The presence of ladders of DNA fragments in the DNA fragmentation assay indicates a biochemical hallmark of intrinsic apoptotic cell death. Altered cell morphology after treatment with the extract demonstrated that cells experienced apoptosis.
Conclusion: The present findings encourage further for the isolation and identifying, of active components present in S. samarangense fruit pulps to understand the mechanism of action in vivo that can make an allowance for developing chemotherapeutic agent against cancer.
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