FORMULATION DEVELOPMENT AND OPTIMIZATION OF NATEGLINIDE-LOADED ETHYL CELLULOSE NANOPARTICLES BY BOX-BEHNKEN DESIGN
Keywords:
Box-Behnken, Drug delivery, Nanoparticles, Nateglinide, Response surface methodology, Solvent evaporation methodAbstract
Objective: Application of nanotechnology in drug delivery system has released leading new areas of research in sustained release of drugs. The objective of the present study is development and optimization of polymeric nanoparticles of Nateglinide (NTG).
Methods: Nateglinide loaded ethyl cellulose (EC) nanoparticles were prepared by the solvent evaporation technique. Response surface methodology (RSM) using the Box-Behnken design was used to optimize the formulation of Nateglinide nanoparticles. The Box-Behnken design consisting of 14 runs, three-factor, three levels and two centre point was used in this study. The particle size, zeta potential and entrapment efficiency of Nateglinide nanoparticles were investigated with respect to three independent variables including stirring speed (X1), time (X2) and surfactant concentration (X3). The optimized nanoparticle is then subjected to characterization studies including morphology, particle size, zeta potential, % Drug Loading (DL) and % Entrapment Efficiency (EE).
Results: Nateglinide nanoparticles under the optimized conditions gave rise to the DL of 14.30±0.27 %, EE of 72.19±0.24 %, mean diameter of 172 nm and zeta potential value of-15.6 mV.
Conclusion: The optimized nanoparticles formulation with improved characteristic properties could be a promising delivery system for Nateglinide.
Â
Downloads
References
Vyas SP, Khar RK. Targeted and controlled drug delivery: Novel carrier system. 1st ed. CBS Publishers and distributors; 2007. p. 363.
Amulyaratna B, Sunit Kumar S. Development and in-vitro, in-vivo evaluation of controlled release, biocompatible nanoparticles. Digest J Nanomater Biostruct 2012;7:217-25.
Daisy P, Jeeva Kani FG. Evaluation of antidiabetic activity of various extracts of cassia Auriculata Linn. bark on streptozotocin-induced diabetic wistar rats. Int J Pharm Pharm Sci 2012;(4, Suppl 4):312-8.
Dhana lekshmi UM, Poovi G, Neelakanta Reddy P. In-vitro observation of repaglinide engineered polymeric nanoparticles. Digest J Nanomater Biostruct 2012;7:1-18.
Jassim ZE, Hussein AA. Formulation and evaluation of clopidogrel tablet incorporating drug nanoparticles. Int J Pharm Pharm Sci 2014;6:838-51.
Kaleemuddin M, Srinivas P. Lyophilized oral sustained release polymeric nanoparticles of nateglinide. AAPS Pharm Sci Tech 2013;14:78-85.
Box GEP, Wilson KB. On the experimental attainment of optimum conditions. J Royal Statistical Soc: Series B 1951;13:1-45.
Box GEP, Hunter JS. Multi-factor experimental design for exploring response surfaces. Ann Math Stat 1957;28:195-241.
Jain S, Saraf S. Influence of processing variables and in-vitro characterization of glipizide loaded biodegradable nanoparticles. Diabetes Metabolic Syndrome: Clinical Research Reviews 2009;3:113-7.
Dhana lekshmi UM, Poovi G, Kishore N, Neelakanta Reddy P. In-vitro characterization and in-vivo toxicity study of repaglinide loaded poly (methyl methacrylate) nanoparticles. Int J Pharm 2010;396:194-203.
Kashanian S, Azandaryani AH, Derakhshandeh K. New surface-modified solid lipid nanoparticles using N‑glutaryl phosphatidylethanolamine as the outer shell. Int J Nanomed 2011;6:2393‑401.
Hoa LTM, Chi NT, Triet NM, Nhan LNT, Chien DM. Preparation of drug nanoparticles by emulsion evaporation method. J Phys: Conf Ser 2009;187:1-4.
Madhavi A, Reddy GS, Suryanarayana MV, Naidu A. Development of a new analytical method for determination of related components in nateglinide. Chromatogra 2008;67:639-45.
Yuan XB, Yuan YB, Jiang W, Liu J, Tian EJ, Shun HM, et al. Preparation of rapamycin-loaded chitosan/PLA nanoparticles for immunosuppression in corneal transplantation. Int J Pharm 2008;349:241-8.
Marcos F, SofÃa N, Karla S, Ana Fernández C, Emilia B. An effective novel delivery strategy of rasagiline for Parkinson’s disease. Int J Pharm 2011;419:271–80.
Ahn JH, Kim YP, Lee YM, Seo EM, Lee KW, Kim HS. Optimization of microencapsulation of seed oil by response surface methodology. Food Chem 2008;107:98-105.
Myers RH, Montgomery DC. Response Surface Methodology. New York: John Wiley and Sons Inc; 1995. p. 700.