IN VITRO ANTIOXIDANT AND ANTICANCER ACTIVITY OF MIMOSA PUDICA LINN EXTRACT AND L-MIMOSINE ON LYMPHOMA DAUDI CELLS
Keywords:
Anticancer, Antioxidant, Daudi, Mimosa pudica, L-Mimosine, HPTLCAbstract
Objective: The present study is an attempt to investigate the antioxidant and anticancer potential of hydroalcoholic extract of Mimosa pudica Linn (Mimosaceae) and L-Mimosine on Daudi cell line.
Methods: The analysis of the standard compound L-Mimosine was ascertained by High Performance Thin Layer Chromatography (HPTLC). Free radical scavenging activity of M. pudica extract and L-Mimosine was also compared using 2, 2-diphenyl–1-picrylhydrazylradical scavenging assay (DPPH). Cell viability and cytotoxicity on Daudi cells were evaluated by trypan blue and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays in a dose and time-dependent manner respectively.
Results: HPTLC analysis showed the presence of amino acids, amines, lipids in the hydroalcoholic extract of M. pudica. Crude hydroalcoholic extract of M. pudica showed antioxidant activity (IC50=103.88 µg/ml) whereas L-Mimosine showed antioxidant activity (IC50=233.06 µM). The crude extract and compound inhibited the proliferation and growth of the Daudi cells through induced cell death. The IC50 value for anticancer activity was found to be 201.65 µg/ml and 86.61 µM at 72 h for M. pudica extract and L-Mimosine respectively.
Conclusion: The results indicated the presence of L-Mimosine in the hydroalcoholic extract of M. pudica. The hydroalcoholic extract and pure compound proved potent inhibitors of cell proliferation, thus manifesting significant antioxidant and anticancer activity.
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Bleyer A, Viny A, Barr R. Cancer in 15-to 29-year-olds by primary site. Oncologist 2006;11:590-601.
Holohan C, Schaeybroeck SV, Long ley DB, Johnston PG. Cancer drug resistance: an evolving paradigm. Nat Rev Cancer 2013;13:714-26.
Cragg GM, Newman DJ. Plants as a source of anticancer agents,â€. J Ethnopharmacol 2005;100:72-9.
Ghani A. Medicinal plants of Bangladesh with chemical constituents and uses. ed 2. Dhaka, Bangladesh; 2003. p. 302-3.
Paranjpe P. Indian medicinal Plants: Forgotten healers. Delhi, India; 1989. p. 155-6.
Thompson JF, Morris CJ, Smith IK. New naturally occurring amino acids. Annu Rev Biochem 1969;38:137-58.
Oppenheim EW, Nasrallah IM, Mastri MG, Stover PJ. Mimosine is a cell-specific antagonist of folate metabolism. J Biol Chem 2000;275:19268-74.
Gilbert DM, Neilson A, Miyazawa H, De Pamphilis ML, Burhans WC. Mimosine arrests DNA synthesis at replication forks by inhibiting deoxyribonucleotide metabolism. J Biol Chem 1995;270:9597-606.
Reno F, Tontini A, Burattini S, Papa S, Falcieri E, Tarzia G. Mimosine induces apoptosis in the HL60 human tumor cell line. Apoptosis 1999;4:469-77.
Zalatnai A, Bocsi J. Mimosine, a plant-derived amino acid induces apoptosis in human pancreatic cancer xenografts. Anticancer Res 2003;23:4007-9.
Chang HC, Weng CF, Yen MH, Chuang LY, Hung WC. Modulation of cell cycle regulatory protein expression and suppression of tumor growth by mimosine in nude mice. Int J Oncol 2000;17:659-65.
Ju H, Hao J, Zhao S, Dixon IM. Antiproliferative and antifibrotic effects of mimosine on adult cardiac fibroblasts. Biochim Biophys Acta 1998;1448:51-60.
Hashiguchi H, Takahashi H. Inhibition of two copper-containing enzymes tyrosinase and dopamine beta hydroxylase, by L-mimosine. Mol Pharmacol 1977;13:362-7.
Hanauske-Abel HM, Park MH, Hanauske AR, Popowicz AM, Lalande M, Folk JE. Inhibition of the G1-S transition of the cell cycle by inhibitors of deoxyhypusine hydroxylation. Biochim Biophys Acta 1994;1221:115-24.
Blois MS, Zhao XY. Antioxidant determinations by the use of stable free redicals. Nature 1985;181:1199-200.
Yamasaki K, Hashimoto A, Kokusenya Y, Miyamoto T, Sato T. Electrochemical method for estimating the antioxidative effects of methanol extracts of crude drugs. Chem Pharm Bull 1994;42:1663-5.
Spenser ID, Notation AD. A synthesis of mimosine. Can J Chem 1962;40:1374-79.
Sharma A, Patel VK. In vitro screening of the antibacterial activity and identification of bioactive compounds from plants against selected Vibrio spp. pathogens. Turk J Biol 2009;33:137-44.
Phillips HJ, Terryberry JE. Counting actively metabolizing tissue cultured cells. Exp Cell Res 1957;13:341-7.
Kuttan R, Bhanumathly P, Nirmala K, George MC. Potential anticancer activity of turmeric (Curcuma longa). Cancer Lett 1985;29:197-202.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and Cytotoxicity assays. J Immunol Methods 1983;65:55-63.
Wilson AP. Cytotoxicity and viability Assay in Animal Cell culture, A Practical Approach. ed 3. New York, NY; 2000. p. 175-219.
Jose J, Sudheesh S, Sumesh Kumar TM, Sony J, Jayadevi Variyar E. A comparative evaluation of anticancer activities of flavonoids isolated from Mimosa pudica, Aloe vera, Phyllanthus niruri against human breast carcinoma cell line (MCF-7) using MTT assay. Int J Pharm Pharm Sci 2014;6:319-22.
Srikanta C, Dibyajyoti S, Swati P. In vitro cytotoxic activities of methanolic extracts of Mimosa pudica. Bull Pharm Res 2012;2:42-5.
Lalande M, Hanauske-Abel HM. A new compound which reversibly arrests T lymphocyte cell cycle near the G1/S boundary. Exp Cell Res 1990;188:117-21.
Linn HB, Falchetto R, Mosca PJ, Shabanowitz J, Hunt DF, Hamlin JL. Mimosine targets serine hydroxymethyltransferase. Biol Chem 1996;271:2548-56.
Tsai WC, Ling KH. Study on the stability constant of some metal ion chelatea of mimosine and 3,4-tihydroxypyridine. J Chin Biochem Soc 1973;2:70-86.
Abbruzzese A, Hanauske AHM, Park MH, Henke S, Folk JE. The active site of deoxyhypusyl hydroxylase: use of catechol peptides and their component chelator and peptide moieties as molecular probes. Biochim Biophys Acta 1991;1077:159-66.
Feldman ST, Schonthal A. Negative regulation of histone H1 kinase expression by mimosine, a plant amino acid. Cancer Res 1994;54:494-8.
Zalatnai A. P-glycoprotein expression is induced in human pancreatic xenografts during treatment with a cell cyclo regulator, mimosine. Pathol Oncol Res 2005;11:164-9.
Mosca PJ, Dijkwel PA, Hamlin JL. The plant amino acid mimosine may inhibit initiation at origins of replication in Chinese hamster cells. Mol Cell Biol 1992;12:4375-83.
Kulp KS, Vulliet PR. Mimosine blocks cell cycle progression by chelating iron in asynchronous human breast cancer cells. Toxicol Appl Pharmacol 1996;139:356–64.
Gilbert DM, Neilson A, Miyazawa H, Depamphilis ML, Gupta HK, Altreja PP. Influence of ferric chloride treated Leucaena leucocephala on metabolism of mimosine and 3-hydroxy-4 (1H)-pyridone in growing rabbits. Anim Feed Sci Technol 1998;74:45-55.
Chen CW, Ho CT. Antioxidant properties of polyphenols extracted from green and black teas. J Food Lipids 1995;2:35-46.