EVALUATION OF LIPOSOMAL GOSSYPIN IN ANIMAL MODELS OF EPILEPSY
Abstract
Objective: Epilepsy is a chronic neurological disorder affecting 1 % of the population worldwide. A number of studies have reported neuroprotective, anticonvulsant and anti-oxidant activity of gossipin a bioflavonoid isolated from Hibiscus vitifolius. The present study was carried out to evaluate the acute effects of liposome entrapped gossypin on Increasing Current electroshock seizures (ICES) test; Pentylenetetrazole (PTZ) induced seizures and status epilepticus in mice.
Methods: Gossypin liposomes were prepared by film hydration technique, and the effect of liposomal Gossypin formulations was studied in two doses i.e. 2.5 mg/kg and 5 mg/kg given per oral on ICES test and PTZ induced seizures in mice. Same doses of the formulation were administered by intravenous route during PTZ induces status epilepticus in mice.
Results: The results indicated that liposome entrapped Gossypin in doses 2.5 mg/Kg and 5 mg/Kg demonstrated significant increase in seizure threshold and latency to generalized seizures in ICES test and PTZ induced seizures respectively. Oxidative stress parameters like malondialdehyde (MDA) and glutathione were estimated in brain tissues in mice. Increased levels of MDA and glutathione were reduced and liposomal Gossypin suppressed the progression of kindling in mice. These results suggest that liposomal Gossypin appears to possess protective activity against kindling in mice.
Conclusion: To conclude, the study supports that liposomal Gossypin offers protection against PTZ kindling in mice. Liposomal Gossypin administration significantly reduced the progression of kindling in mice therefore it could be a promising candidate to control both development of seizures and oxidative stress during epilepsy.
Keywords: Epilepsy, Gossypin, Liposomes, ICES and PTZ induced seizures, Status epilepticus
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