GENETIC SUSCEPTIBILITY OF HCV RNA IN VARIOUS ISONYM GROUPS OF PUNJAB, PAKISTAN
Keywords:
HCV, Genotypes, Genetic, PolymorphismAbstract
Objective: To uncover the ecology and genetic factors affecting the susceptibility of infectious diseases of HCV RNA and to understand the role of genetic characters in the outcome and pathogenesis of hepatitis C virus (HCV) infection.
Methods: A total of 349 patients with chronic hepatitis have been included in this genetic susceptibility study. The infected individuals were diagnosed anti-HCV positive and then confirmed by HCV Polymerized Chain Reaction (PCR) qualitative test. All these selected patients had been diagnosed for HCV and taken the standard treatment of interferon and ribavirin from 20 w to 36 w. The end product of PCR was confirmed by gel electrophoresis. The following restriction enzymes Ear I, Rsa 1 and Mae III were used to digest the PCR product. This study focused only on patients who were non-responsive and had relapsed from conventional therapy.
Results: Our study proposes that both the interleukin genes IL-10 and IL-28B are found common in two major castes of the Punjab. We determined that HCV genotype 3a is very common (84.0%) among the group of responders whereas genotype 1a is more familiar in relapse (66.2%) as well as non-responders (54.0%). Excluding Genotype 4, the rest five main genotypes, namely 1a (61.40%), 2a (0.50%), 2b (20.00%), 3a (13.70%) and an untraceable (4.40%) were found among the 12 different castes/tribes ethnic groups I Pakistan.
Conclusion: To better manage and improve the fact behind understanding human susceptibility to HCV infection, a cohort study is beneficial.
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References
Choo QL, Kuo G, Weiner AJ, Overby LR, Bradley DW, Houghton M. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 1989;244:359-62.
Kapoor A, Simmonds P, Gerold G, Qaisar N, Jain K, Henriquez JA, et al. Characterization of a canine homolog of hepatitis C virus. Proc Natl Acad Sci U S A 2011;108:11608-13.
Beltrami EM, Williams IT, Shapiro CN, Chamberland ME. Risk and management of blood-borne infections in health care workers. Clin Microbiol Rev 2000;13:385-407.
Leone N, Rizzetto M. Natural history of hepatitis C virus infection: from chronic hepatitis to cirrhosis, to hepatocellular carcinoma. Minerva Dietol Gastroenterol 2005;51:31-46.
Gretch DR. Diagnostic tests for hepatitis C. Hepatology 1997;26:43S-7S.
Pawlotsky J. Measuring hepatitis C viremia in clinical samples: can we trust the assays? Hepatology 1997;26:1-4.
Duvoux C, Pawlotsky JM, Cherqui D, Tran van Nhieu J, Metreau JM, Fagniez PL, et al. Serial quantitative determination of hepatitis C virus RNA levels after liver transplantation. A useful test for diagnosis of hepatitis C virus reinfection. Transplantation 1995;60:457-61.
Pawlotsky JM, Roudot-Thoraval F, Bastie A, Darthuy F, Remire J, Metreau JM, et al. Factors affecting treatment responses to interferon-alpha in chronic hepatitis C. J Infect Dis 1996;174:1-7.
Urdea M. Quantification of hepatitis C virus RNA: clinical applications of the branched DNA assay. Groupe Français d'Etudes Moléculaire des Hépatites (GEMHEP) John Libbey Eurotext. Paris 1997;1:73-8.
Arif F FJ, Hamid A. Awareness among parents of children with thalassemia major. J Pak Med Assoc 2008;58:621-4.
http://www.patient.co.uk/doctor/hepatitis-c-pro. [Last accessed on 10 Aug 2015].
BJ S. Is interleukin-10 gene polymorphism a predictive marker in HCV infection? Cytokine Growth Factor Rev 2012;23:47-59.
Hedrich CM, Bream JH. Cell type-specific regulation of IL-10 expression in inflammation and disease. Immunol Res 2010;47:185-206.
Aslamkhan M: Cultural Consanguinity. Proc. In: 3rd. Internat Conf Medical and Community Genetics. Chandigarh, India; 2008. p. 71.
Aslamkhan M. Primary prevention of disability. Mother Child 1983;20:9-14.
Ibbetson D EM, Rose H. A glossery to the tribes and casts of the punjab and North west frontier province. In: Punjab in Lahore Printed by the superintendent, Government Printing; 1911.
Saeed-ur-Rahman Z-u-RA, Arif-ullah Khan, Abdul Haleem Shah, Abdur Rahman, Inayat Gul. Malaria among the students of religious schools of bannu of bannu district khyber paktunkhea pakistan. Pakistan J Zoology 2012;44:959-62.