ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF METHANOLIC EXTRACT OF BAUHINIA RACEMOSA

Authors

  • Pritipadma Panda School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan University, Kalinga Nagar, Ghatikia, Bhubaneswar-03, Odisha, India
  • Abhisek Pal School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan University, Kalinga Nagar, Ghatikia, Bhubaneswar-03, Odisha, India
  • Laxmidhar Maharana School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan University, Kalinga Nagar, Ghatikia, Bhubaneswar-03, Odisha, India
  • Goutam Ghosh School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan University, Kalinga Nagar, Ghatikia, Bhubaneswar-03, Odisha, India

Abstract

Objective: To evaluate antimicrobial and in vitro [s1] antioxidant activities of methanolic extract of Bauhinia racemosa leaf.

Methods: The antimicrobial activity was determined by the agar-well diffusion method. The antioxidant potential was evaluated by using various in vitro [s2] methods such as 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2, 2-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), phospho molybdenum reduction assay and ferric (Fe 3+) reducing power assays.

Results: The maximum inhibition zone (26 mm) was found against Proteus mirabilis while lowest inhibition zone (20 mm) was observed against Acinetobacter baumannii.

The DPPH and ABTS were significantly inhibited by methanolic extract of Bauhinia racemosa leaf with IC50 of 9 μg/ml and 550μg/ml respectively, whereas methanolic extract of leaves showed good antioxidant potential using ferric reducing power assay (1.896[s3]  and phosphomolybdate assay (108.12 mg AAE/g) methods.

Conclusion: The results of present study showed that Bauhinia racemosa leaf possesses high potential antimicrobial and antioxidant activity and could be a potential source of natural antioxidant that could have great importance as therapeutic agents in preventing oxidative stress-related degenerative diseases.

Keywords: Bauhinia racemosa, Antimicrobial, In vitro [s4] antioxidant, DPPH, ABTS, FRPA.

 [s1]italic

 [s2]italic

 [s3]IC50 or what?

 [s4]italic

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Author Biographies

Pritipadma Panda, School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan University, Kalinga Nagar, Ghatikia, Bhubaneswar-03, Odisha, India

Dept. of Pharmacognosy, Research Scholar

Abhisek Pal, School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan University, Kalinga Nagar, Ghatikia, Bhubaneswar-03, Odisha, India

Department of Pharmacology, Assistant Professor

Laxmidhar Maharana, School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan University, Kalinga Nagar, Ghatikia, Bhubaneswar-03, Odisha, India

epartment of Pharmacology, Assistant Professor

Goutam Ghosh, School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan University, Kalinga Nagar, Ghatikia, Bhubaneswar-03, Odisha, India

Department of Pharmacognosy, Assistant Professor

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Published

01-02-2016

How to Cite

Panda, P., A. Pal, L. Maharana, and G. Ghosh. “ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF METHANOLIC EXTRACT OF BAUHINIA RACEMOSA”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 2, Feb. 2016, pp. 277-81, https://mail.innovareacademics.in/journals/index.php/ijpps/article/view/9127.

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