ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF METHANOLIC EXTRACT OF BAUHINIA RACEMOSA
Abstract
Objective: To evaluate antimicrobial and in vitro [s1]Â antioxidant activities of methanolic extract of Bauhinia racemosa leaf.
Methods: The antimicrobial activity was determined by the agar-well diffusion method. The antioxidant potential was evaluated by using various in vitro [s2]Â methods such as 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2, 2-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), phospho molybdenum reduction assay and ferric (Fe 3+) reducing power assays.
Results: The maximum inhibition zone (26 mm) was found against Proteus mirabilis while lowest inhibition zone (20 mm) was observed against Acinetobacter baumannii.
The DPPH and ABTS were significantly inhibited by methanolic extract of Bauhinia racemosa leaf with IC50 of 9 μg/ml and 550μg/ml respectively, whereas methanolic extract of leaves showed good antioxidant potential using ferric reducing power assay (1.896[s3] and phosphomolybdate assay (108.12 mg AAE/g) methods.
Conclusion: The results of present study showed that Bauhinia racemosa leaf possesses high potential antimicrobial and antioxidant activity and could be a potential source of natural antioxidant that could have great importance as therapeutic agents in preventing oxidative stress-related degenerative diseases.
Keywords: Bauhinia racemosa, Antimicrobial, In vitro [s4] antioxidant, DPPH, ABTS, FRPA. [s1]italic
 [s2]italic
 [s3]IC50 or what?
 [s4]italic
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References
Kedare SB, Singh RP. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol 2011;48:412-22.
Sahoo S, Ghosh G, Das D, Nayak S. Phytochemical investigation and in vitro antioxidant activity of an indigenous medicinal plant Alpinia nigra B. L. Burtt. Asian Pac J Trop Biomed 2013;3:871-6.
Ghosh G, Sahoo S, Das D, Dubey D, Padhy RN. Antibacterial and antioxidant activities of methanol extract and fractions of Clerodendrum viscosum Vent. Leaves. Indian J Nat Prod Resour 2014;5:134-42.
Kirtikar KR, Basu BA. Indian medicinal plants. 2nd ed. Periodical Experts Book Publication, New Delhi; 1976. p. 842-4.
Prakash A, Khosa RL. Chemical studies on Bauhinia racemosa. Curr Sci 1976;45:705-7.
El-Hossary GA, Selim MA, Sayed AE, Khaleel AE. Study of the flavonoid content of Bassia muricata and Bauhinia racemosa. Bull Faculty Pharm Cairo University 2000;38:93-7.
Anjaneyulu ASR, Reddy AVR, Reddy DSK, Ward RS, Adhikesavalu D, Cameron TS. A new dibenzo (2,3-6,7) oxepin derivative from Bauhinia racemosa. Tetrahedron 1984;40:4245-52.
Harborne JB. Phytochemical methods. 3rd ed. London, Chapman and Hall; 1984.
Nayak N, Rath SN, Mishra MP, Ghosh G, Padhy RN. J Acute Dis 2013;2:270-6.
Panda P, Singh S, Pal A, Dash P, Ghosh G. Antimicrobial and immunomodulatory activities of methanolic extract of Bauhinia vahlii. Res J Pharm Biol Chem Sci 2015;6:655-60.
Memarpoor-Yazdi M, Asoodeh A, Chamani J. A novel antioxidant and antimicrobial peptide from hen egg white lysozyme hydrolysates. J Funct Foods 2012;4:278–86.
Pellegrini N, Serafini M, Colombi B, Rio DD, Salvatore S. Total antioxidant capacity of plant food, beverages and oils consumed in Italy assessed by three different in vitro assays. J Am Coll Nutr 2003;133:2812-9.
Oyaizu M. Studies on the product of browning reaction prepared from glucose amine. Jpn J Nutr 1986;44:307-15.
Prieto P, Pineda M, Anguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a Phosphomolybdenum complex: specific application to the determination of Vitamin E. Anal Biochem 1999;269:337-41.
Kumarasamy KK, Toleman MA, Walsh TR, Bangari J, Butt F, Balkrishnan R, et al. Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study. Lancet Infect Dis 2010;10:597.
Ghosh A, Das BK, Roy A, Mandal B, Chandra G. Antibacterial activity of some medicinal plant extracts. J Nat Med 2008;62:259.
Rameskumar A, Sivasudha T. In vitro antibacterial and antioxidant activity of an aqueous and methanolic extract of Mollugo nudicaulis Lam. Leaves Asian J Trop Biomed 2012;S895-S900.
Thayalini T, Thevanesam V, Kathirgamanathar S, Gamage TM. Antibacterial activity and preliminary screening of phytochemicals of the whole plant of Enicostemma littorale. Trop Agric Res 2012;24:99–105.
Jamuna S, Paulsamy S, Karthika K. Screening of in vitro antioxidant activity of methanolic leaf and root extracts of Hypochaeris radicata L. J Appl Pharm Sci 2012;2:149-54.
Silva CG, Herdeiro RS, Mathias CJ, Panek AD, Silveira CS, Rodrigues VP, et al. Evaluation of antioxidant activity of Brazilian plants. Pharmacol Res 2005;52:229-33.
Sharma S, Pal Vig A. Evaluation of in vitro antioxidant properties of methanol and aqueous extracts of Parkinsonia aculeata L. leaves, Sci World J 2013;2013:1-7.
Sahreen S, Khan MR, Khan RA. Evaluation of antioxidant activities of various solvent extracts of Carissa opaca fruits. Food Chem 2010;122:1205-11.
Saha MR, Hasan SMR, Akter R, Hossain MM, Alam MS, Alam MA, et al. In vitro free radical scavenging activity of methanol extract of the leaves of Mimusops elengi Linn. Bangl J Vet Med 2008;6:197–202.
Rajamanikandan S, Sindhu T, Durgapriya D, Sophia D, Ragavendran P, Gopalakrishnan VK. Radical scavenging and antioxidant activity of ethanolic extract of Mollugo nudicaulis by in vitro assays. Indian J Pharm Edu Res 2011;45:310-6.
Ravisankar N, Sivaraj C, Seeni S, Joseph J, Raaman N. Antioxidant activites and phytochemical analysis of methanol extract of leaves of Hypericum hookerianum. Int J Pharm Pharm Sci 2014;6:456-60.