UMBELLIFERONE EXTENUATES THE ABNORMALITIES IN LIPID METABOLISM DURING D-GALACTOSAMINE AND LIPOPOLYSACCHARIDE INDUCED FULMINANT HEPATIC FAILURE IN RATS.

Authors

  • Tamilarasi Sasivarnam
  • Pramila Chengalvarayan Subramani
  • Indumathi Selvanathan
  • Devaki Thiruvengadam Department of Biochemistry, University of Madras, Guindy Campus, Chennai 600025, TN, India

Abstract

Objective: Coumarins and coumarin-related compounds are a plentiful source of potential drugs candidate in relation to its safety and efficacy. Hence, the present study was designed to evaluate the protective nature of Umbelliferone (UMB), a coumarin derivative on alterations in lipid metabolism during D-Galactosamine (D-GalN) and Lipopolysaccharides (LPS) induced Fulminant hepatic failure (FHF).

Methods: Male wistar rats were challenged with a single intraperitoneal injection of D-GalN/LPS, and the protective nature of UMB was examined with reference to Total Cholesterol, Phospholipids, Triglycerides, free fatty acids(plasma, liver, and kidney), lipid metabolizing enzymes and lipoprotein fraction.

Results: Anomalies in lipid status, lipid metabolizing enzymes and lipoprotein fractions induced by D-GalN/LPS was restored to near normal levels upon pretreatment with UMB which was further confirmed by Transmission electron microscopic observations.

Conclusion: The outcome of the above findings evidenced the protective nature of UMB on alterations in lipid metabolism during D-GalN/LPS induced FHF in rats.

Keywords: Umbelliferone, D-Galactosamine, Lipopolysaccharides (LPS), Lipid metabolism, Liver failure, Lipid metabolizing enzymes, Lipoproteins, Transmission electron microscope.

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References

Gupta RK, Singh RK, Swain SR, Hussain T, Rao CV. The anti-hepatotoxic potential of hedyotis corymbosa against D-galactosamine-induced hepatopathy in experimental rodents. Asian Pac J Trop Biomed 2012;2:S1542–7.

Shin JW, Wang JH, Park HJ, Choi MK, Kim HG, Son CG. Herbal formula CGX ameliorates LPS/D-Galactosamine-induced hepatitis. Food Chem Toxicol 2011;49:1329–34.

Gong X, Luo F, Zhang L, Li H, Wu M, Li X, et al. Tetrandrine attenuates lipopolysaccharide-induced fulminant hepatic failure in D-galactosamine-sensitized mice. Int Immuno-pharmacol 2010;10:357–63.

Sayed RH, Khalil WKB, Salem HA Sanaa, El-sayeh BM. Science direct sulforaphane increases the survival rate in rats with fulminant hepatic failure induced by D-galactosamine and lipopolysaccharide. Nutr Res 2014;34:982–9.

Wang Y, Li Y, Xie J, Zhang Y, Wang J, Sun X, et al. Protective effects of probiotic lactobacillus casei zhang against endotoxin and d-galactosamine-induced liver injury in rats via anti-oxidative and anti-inflammatory capacities. Int Immuno-pharmacol 2013;15:30–7.

Zhang L, Li H-Z, Gong X, Luo F-L, Wang B, Hu N, et al. Protective effects of Asiaticoside on acute liver injury induced by lipopolysaccharide/D-galactosamine in mice. Phytomedicine 2010;17:811–9.

Jain PK, Joshi H. Coumarin: chemical and pharmacological profile. J Appl Pharm Sci 2012;2:236–40.

Venugopala KN, Rashmi V, Odhav B. Review on natural coumarin lead compounds for their pharmacological activity. BioMed Research International 2013. doi.org/ 10.1155/ 2013/963248. [Article in Press]

Choi J, Lee KT, Ka H, Jung WT, Jung HJ, Park HJ. Constituents of the essential oil of the Cinnamomum cassia stem bark and the biological properties. Arch Pharm Res 2001;24:418–23.

Bourgaud F, Hehn A, Larbat R, Doerper S, Gontier E, Kellner S, et al. Biosynthesis of coumarins in plants: a major pathway still to be unravelled for cytochrome P450 enzymes. Phytochem Rev 2006;5:293–308.

Rosselli S, Maggio AM, Faraone N, Spadaro V, Morris-Natschke SL, Bastow KF, et al. The cytotoxic properties of natural coumarins isolated from roots of Ferulago campestris (Apiaceae) and of synthetic ester derivatives of aegelinol. Nat Prod Commun 2009;4:1701–6.

Hoult JR, Forder RA, de las Heras B, Lobo IB, Paya M. Inhibitory activity of a series of coumarins on leukocyte eicosanoid generation. Agents Actions 1994;42:44–9.

Egan D, James P, Cooke D, O’Kennedy R. Studies on the cytostatic and cytotoxic effects and mode of action of 8-nitro-7-hydroxycoumarin. Cancer Lett 1997;118:201–11.

Dhankhar S, Ruhil S, Balhara M, Dhankhar S, Chhillar AK. Aegle marmelos (Linn.) correa: a potential source of phytomedicine; 2011;5:1497–507.

Wu FJ SS. Analysis and processing of Chinese herbal drugs: the study of fructus aurantii Immaturus. Chin Pharm J 1992;44:257–63.

Dean FM. Naturally occurring oxygen ring compounds. Butterworths, London; 1963.

Hoult JR, Paya M. Pharmacological and biochemical actions of simple coumarins: natural products with therapeutic potential. Gen Pharmacol 1996;27:713–22.

Ramesh B, Pugalendi KV. Antihyperlipidemic and antidiabetic effects of umbelliferone in streptozotocin diabetic rats. Yale J Biol Med 2005;78:189–96.

Ramesh B, Pugalendi KV. Antihyperglycemic effect of umbelliferone in streptozotocin-diabetic rats. J Med Food 2006;9:562–6.

Jayakumar S, Hari N. Bhilwade RCC. Barc newsletter suppression of radiation-induced DNA damage and apoptosis in hematopoietic cells of mice by umbelliferone; 2013. p. 327–31.

Lino CS, Taveira ML, Viana GSB, Matos FJA. Analgesic and antiinflammatory activities of Justicia pectoralis Jacq and its main constituents: coumarin and umbelliferone. Phyther Res 1997;11:211–5.

Ramalingam R, Vaiyapuri M. Effects of umbelliferone on lipid peroxidation and antioxidant status in diethylnitrosamine-induced hepatocellular carcinoma. J Acute Med 2013;3:73–82.

Namisaki T, Yoshiji H, Kuriyama S, Kojima H, Yoshii J, Ikenaka Y, et al. A potent angiogenic factor, vascular endothelial growth factor, improves the survival of the on-going acute hepatic failure in rats. Hepatol Res 2006;35:199–203.

Parekh AC, Jung DH. Cholesterol determination with ferric acetate-uranium acetate and sulfuric acid-ferrous sulfate reagents. Anal Chem 1970;42:1423-7.

Fiske CH, Subbarow Y. The colorimetric determination of phosphorus. J Biol Chem 1925;66:375–400.

Foster LB, Dunn RT. Stable reagents for determination of serum triglycerides by a colorimetric hantzsch condensation method. Clin Chem 1973;19:338–40.

Hron WT, Menahan LA. A sensitive method for the determination of free fatty acids in plasma. J Lipid Res 1981;22:377–81.

Korn ED. Clearing factor, a heparin-activated lipoprotein lipase. I. Isolation and characterization of the enzyme from normal rat heart. J Biol Chem 1955;215:1–14.

Hitz J, Steinmetz J, Siest G. Plasma lecithin: cholesterol acyltransferase--reference values and effects of xenobiotics. Clin Chim Acta 1983;133:85–96.

Schmidt FH, Stork H, Dahl K. Methods in enzymatic analysis. New York: Acad Press; 1974. p. 819.

Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972;18:499–502.

Taniguchi H, Yomota E, Nogi K, Onoda Y. Effects of anti-ulcer agents on ethanol-induced gastric mucosal lesions in D-galactosamine-induced hepatitis ratSDrug Res (Stuttg) 2004;52:600–4.

Lim HK, Kim HS, Choi HS, Oh S, Jang CG, Choi J, et al. Effects of acetylbergenin against D-galactosamine-induced hepatotoxicity in rats. Pharmacol Res 2000;42:471–4.

McIntyre N, Rosalki S. Biochemical investigations in the management of liver disease. In: Prieto J, Rodés J, Shafritz D. eds. Hepatobiliary Dis. SE-2. Springer Berlin Heidelberg; 1992. p. 39–71.

Cartwright CK, Ragland JB, Weidman SW, Sabesin SM. Alterations in lipoprotein composition associated with galactosamine-induced rat liver injury. J Lipid Res 1982;23:667–79.

Ray E, Bellini F, Stoudt G, Hemperly S, Rothblat G. Influence of lecithin: cholesterol acyltransferase on cholesterol metabolism in hepatoma cells and hepatocytes. Biochim Biophys Acta 1980;617:318–34.

Shivashangari KS, Ravikumar V, Vinodhkumar R, Abdul S Sheriff, Devaki T. Hepatoprotective potential of Lycopene on D-Galactosamine/lipopolysaccharide induced hepatitis in rats. Pharmacologyonline 2006;2:151–70.

Pushpavalli G, Veeramani C, Pugalendi KV. Influence of chrysin on hepatic marker enzymes and lipid profile against D-galactosamine-induced hepatotoxicity rats. Food Chem Toxicol 2010;48:1654–9.

Kramer RM, Sharp JD. Structure, function and regulation of Ca2+-sensitive cytosolic phospholipase A2 (cPLA2). FEBS Lett 1997;410:49–53.

Briddon S, Beck SA, Tisdale MJ. Changes in activity of lipoprotein lipase, plasma free fatty acids and triglycerides with weight loss in a cachexia model. Cancer Lett 1991; 57:49–53.

Sabesin SM, Kuiken LB, Ragland JB. Lipoprotein abnormalities in galactosamine hepatitis: a model of experimental lecithin: cholesterol acyl transferase deficiency. Scand J Clin Lab Invest Suppl 1978;150:187–93.

Black DD, Freeman MR, Sabesin SM. Lipoprotein lipase and hepatic lipase deficiencies associated with impaired chylomicron clearance in D-(+) galactosamine hepatitis. Metabolism 1982;31:620–6.

Annema W, Tietge UJF. Role of hepatic lipase and endothelial lipase in high-density lipoprotein-mediated reverse cholesterol transport. Curr Atheroscler Rep 2011;13:257–65.

Franceschini G. Epidemiologic evidence for high-density lipoprotein cholesterol as a risk factor for coronary artery disease. Am J Cardiol 2001;88:9N–13N.

Katan MB, Grundy SM, Jones P, Law M, Miettinen T, Paoletti R. Efficacy and safety of plant stanols and sterols in the management of blood cholesterol levels. Mayo Clin Proc 2003;78:965–78.

Sathivel A, Raghavendran HRB, Srinivasan P, Devaki T. Anti-peroxidative and anti-hyperlipidemic nature of Ulva lactuca crude polysaccharide on D-galactosamine induced hepatitis in rats. Food Chem Toxicol 2008;46:3262–7.

Published

01-03-2016

How to Cite

Sasivarnam, T., P. C. Subramani, I. Selvanathan, and D. Thiruvengadam. “UMBELLIFERONE EXTENUATES THE ABNORMALITIES IN LIPID METABOLISM DURING D-GALACTOSAMINE AND LIPOPOLYSACCHARIDE INDUCED FULMINANT HEPATIC FAILURE IN RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 3, Mar. 2016, pp. 79-84, https://mail.innovareacademics.in/journals/index.php/ijpps/article/view/9331.

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