EXTRACTION AND SUGAR COMPOSITION OF MUCILAGE IN URGINEA INDICA/DRIMIA INDICA (ROXB) KUNTH HYACINTHACEAE
Abstract
Objective: Urginea indica (U. indica) Kunth. Hyacinthaceae has high mucilage content; the aim of the study is to isolate the mucilage and to analyze the sugar present in mucilage and its Physicochemical properties.
Methods: The mucilage of U. indica extracted from the dried powder from bulbs of Urginea indica using Acetone Precipitation method. HPLC analysis has been made to see the presence of sugars in mucilage. The composition of polysaccharide analyzed based on the standard hydrolysis procedure using hydrochloric, sulfuric, and trifluoroacetic acid (TFA) at elevated temperature.
Results: The percentage yield of mucilage was found to be 4 %. The HPLC analysis revealed the presence of sugar such as Sucrose, Maltose, Fructose and Galactose in mucilage sample among which maltose was found to be higher with 21.84 µg/ml followed by Fructose (1.90 µg/ml), Galactose (2.09 µg/ml) and sucrose (0.82 µg/ml).
Conclusion: The present investigation showed that U. indica mucilage has high pharmaceutical significance with the presence of high amount of maltose, which makes it an attractive source of carbohydrate.
Keywords: Urginea indica, Mucilage, Sugar, Acetone Precipitation method, HPLC, Maltose
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