POTENCY OF TURMERIC RHIZOME DECOCTION ON SPERM INFERTILITY OF MALE MICE TO SUCCEED FAMILY PLANNING PROGRAM IN WEST JAVA SOCIETY
Abstract
Objective: The study aimed to evaluate the effect of turmeric rhizome decoction on sperm fertility of male mice.
Methods: Ten male mice were divided randomly into two groups, control, and tested groups. The control and the tested groups were given water as vehicle and turmeric rhizome decoction at the dose 517.4 mg/kg body weight, respectively for 30 d. The mice were put under anesthesia by urethane, and the percentage of sperm motility, morphology, and sperm concentration were calculated, respectively. The results were compared with seminiferous tubule cell images.
Results: The rapid progressive sperm motility of the control and the tested groups were 53±6.4 % and 13±3.6 %, respectively; the difference was significant (P<0.001). The normal sperm morphology in both the control and the tested groups were 57.8±5.76 % and 40.8±2.72 %, respectively; the difference was significant (P<0.01). However, the differences of sperm concentration of both groups were not considered significant. Seminiferous tubule cell images of the tested group showed disruption of sertoli cells along with the present of cellular vacuolization and pyknotic nuclei.
Conclusion: Intake of the turmeric rhizome decoction at the dose of 517.4 mg/kg-bw in mice has a significant effect in decreasing the sperm motility and morphology. The decoction caused sperm abnormality or asthenoteratozoospermia. The habit of consuming turmeric decoction can succeed family planning program.
Keywords: Asthenoteratozoospermia, Sperm infertility, Curcuma longa L., Turmeric, Decoction of turmeric rhizome
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References
Ministry of Health of the Republic of Indonesia. Situation and analysis of family planning. Jakarta (Indonesia): Center of data and information Kemenkes Press; 2014.
Dalimartha S. Atlas of Indonesia medicinal plants. 6th ed. Jakarta (Indonesia): Pustaka Bunda; 2008.
Dhianawaty D, Martiana A, Surialaga S. Effect of Curcuma longa L. rhizome decoct on glucose absorption level in the intestine of the male rat of Wistar strain. Int J Pharm Pharm Sci 2014;4:532-53.
Wickenberg J, Ingemansson SL, Hlebowicz J. Effects of Curcuma longa (turmeric) on postprandial plasma glucose and insulin in healthy subjects. Nutr J 2010;43:1-5.
Kamel R, Hashim AA, Ali SAE-M. Palliative effect of curcumin on STZ-induced diabetes in rats. Int J Pharm Pharm Sci 2014;6 Suppl 2:558-63.
Kumar A, Purwar B, Shrivastava A, Pandey S. Effects of curcumin on the intestinal motility of albino rats. Indian J Physiol Pharmacol 2010;3:284-8.
Purwar B, Shrivastava A, Arora N, Kumar A, Saxena Y. Effects of curcumin on the gastric emptying of albino rats. Indian J Physiol Pharmacol 2012;2:168-73.
Soebadi RDH, Pawana IPA. Effect of oral curcumin and immobilization on the diameter of skeletal muscle fibers in Rattus norvegicus. Folia Medica Indonesiana 2008;1:30-4.
Samsudin S, Panigoro R. Comparison of antioxidant activity between decoction of dried Curcuma longa L., and Curcuma xanthorrhiza Roxb. Int J Res Phytochem Pharmacol 2013;1:27-30.
Prasad S, Aggarwal BB. Turmeric, the golden spice. In: Benzie IFF, Galor SW. editors. Herbal medicine, Biomolecular and clinical aspects. 2nd ed. Boca Raton (FL): CRC Press LLC; 2011. p. 263-88.
Satpathy R, Guru RK, Behera R. Evaluation of anti-influenza activity of curcumin derivates by docking and pharmacophore modeling approach. Int J Pharm Pharm Sci 2012;4 Suppl 1:469-73.
Sharaf HA, Morsy FA, Shaffie NM, Shennawy. Histological and histochemical study on the protective effect of curcumin on ultraviolet irradiation-induced testicular damage in albino rats. J Cytol Histol 2012;6:1-8.
Soleimanzadeh A, Saberivand A. Effect of curcumin on rat sperm morphology after the freeze-thawing process. Veterinary Research Forum 2013;3:185-9.
Sneha P, Ramtej V. Protective effect of curcumin on diethanolamine-induced toxic effects on human spermatozoa: an in vitro study. Int Res J Biol Sci 2014;7,34-9.
Awasthi PK, Dixit SC. The chemical composition of Curcuma longa leaves and rhizome oil from the plains of Northern India. J Young Pharm 2009;4:312-6.
WHO. Laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 4rd ed. Cambridge [UK]: Cambridge University Press; 1999.
Junqueira LC, Carneiro J. Male reproductive system. In: Maley J, Lebowitz H, Boyle PJ. editors. Basic histology text & atlas. 11th ed. New York: McGraw-Hill; 2005. p. 418-23.
Berek JS. Infertility. In: Burney RO, Schust DJ, Yao MWM. Editors. Gynaecology. 14th ed. Philadelphia: Lippincott Williams and Wilkins; 2007. p. 1193-5.
Guyton AC, Hall JE. Reproductive and hormonal function of the male. In: Schmitt W, Gruliow R. editors. Medical physiology textbook. 11th ed. Philadelphia: Elsevier Saunders; 2006. p. 996-9.