DETECTION OF BACTERIAL CONTAMINANTS IN READY-TO-DRINK BEVERAGE (ZOBO) SOLD ON FEDERAL UNIVERSITY DUTSE CAMPUS, NORTHWEST NIGERIA
DOI:
https://doi.org/10.22159/ijs.2024.v12i1.50136Keywords:
Zobo, Ready-to-drink, Total viable count, Coliform count, Federal university dutseAbstract
Introduction: Consumption of ready-to-drink beverages is a norm in the world over. However, the presence of total coliform in any beverage renders it unfit for human consumption.
Objectives: Due to non-existence of scientific data on the safety status of the ready-to-drink Zobo which is a popular local beverage, we did conduct this study to assess the bacteriological quality of the lots sold to consumers on the campus of Federal University Dutse (FUD), Nigeria.
Methods: Purposive sampling was employed to sample nine (9) locally packaged Zobo in three (3) different outlets; FUD Bakery (BAK) where three (3) were assayed (BAKA, BAKB, and BAKC), FUD Backside (BS) where three (3) samples were assayed (BSA, BSB, and BSC), and Morocco Girls’ hostel (MGH) three (3) samples were assayed (MGHA, MGHB, and MGHC). Standard methods were used to assay the sampled Zobo.
Results: Results obtained indicate that samples from MGH (8.0×10⁴ CFU/mL) had the lowest total viable count (TVC), while samples from BAK (2.8×10⁷ CFU/mL) recorded the highest TVC. However, samples assayed from MGH recorded no total coliform count. The number of bacterial colonies recorded in BAKA and BAKB was not significantly different from each other (p>0.05). However, the number of bacterial colonies recorded from MGHA was significantly different from those obtained in MGHB and MGHC (p<0.05).
Conclusion: Due to the results obtained, we can conclude that Zobo samples assayed in the sampling points were not fit for human consumption during the conduct of our study.
References
Acharya T. Serial Dilution Method for Estimating Viable Count of Bacteria. 2022. Retrieved on Dec 09 2023. Available from: https://micro beonline.com/serial-dilution-method/
Adeoye BK, Oyewole OB, Idowu MA, Obadina AO, Ani IF, Ngozi EO. Effect of spices on the microbiological quality of Hibiscus sabdariffa (zobo) drink and molecular characterization of the associated spoilage organisms. African Journal of Biotechnology, 2018;17:1057-1061.
American Public Health Association (APHA). Standard methods for examination of water. American Public Health Association, American Water Works Association and Water Pollution Control Federation. 2005;5-17. 20th edn. Washington DC, USA.
Amusa NA, Ashaye OA, Aiyegbajo AA, Oladapo MO, Oni MO. Microbiological and nutritional quality of hawked sorrel drink (Soborodo) widely consumed and notable. Nigeria Journal of Food Agriculture and Environment, 2005;3:47-50.
Aryal S. Water Quality Analysis by Most Probable Number (MPN). 2018. Retrieved on Dec 09 2023. Available from: https://microbenotes.com/ water-quality-analysis-by-most-probable-number-mpn/.
Ayandele AA. Microbiological analysis of hawked kanun and Zobo drink within LAUTECH campus, Ogbomoso, Oyo State Nigeria. IOSR Journal of Environmental Science, Toxicology and Food Technology, 2015; 9:52-56.
Balakarami AU, Umar A, Mustafa AI. Microbiological Analysis of Drinking water in Maiduguri Metropolis, Nigeria. International Journal of Research, 2016;3. Available from: https://journals.pen2print.org/index.php/ijr/rt/printerFriendly /6603/0.
Bamishaiye EI, Olayemi FF, Bamishaiye OM. Effect of boiling time on minerals and vitamin C content of three varieties of Hibiscus sabdariffa drink in Nigeria. World Journal of Agricultural Science, 2011;7:62-67.
Barrow GI., Feltham RKA. Cowan and Steel's Manual for the Identification of Medical Bacteria. Cambridge University Press Third Edition. 1993;52-101.
Braide W, Oranusi S, Peter-Ikechukwu AI. Prospective in the Hurdle Techniques in the Preservation of a Non Alcholic Beverage, Zobo. African Journal of Food Science and Technology, 2009;3:46-52.
Bristone C, Mariyam K, Ogori AF, Badau MH, Joeguluba O. Microbial quality evaluation of Zobo drink sold in University of Maiduguri. Food Science and Nutrition Technology, 2018;3:1-9.
Bulakarima AU, Bello HS, Isa MA, Abbas MI. Isolation and Identification of Microorganisms from Locally Prepared Zobo Drink in Konduga Town, Borno State, Nigeria. Asian Journal of Research in Biological and Pharmaceutical Sciences, 2017;5:117- 122.
Chessbrough M. District Laboratory Practice in Tropical Countries. 2006;Chapter 7:38-158. Part II. 2nd Ed. New York: Cambridge University Press.
Dogan B, Boor KJ. Genetic diversity and spoilage potentials among Pseudomonas spp. isolated from fluid milk products and dairy processing plants. Applied Environmental Microbiology, 2003;69:130-138. Doi:10.1128/AEM.69.1.130-138.2003.
Egbere OJ, Anuonye JC, Challom PF, Kpara PV. Effect of Some Preservation Techniques on the Quality and Storage Stability of Zobo Drink: A Nigerian Non-alcoholic Beverage from Hibiscus Sabdariffa. Journal of Food Technology, 2007;5:225-228.
Ehsemokha U. Zobo Drink (Hibiscus Tea): Health benefits, side effects, nutritional content and preparation. 2020. Retrieved on Dec 09 2023. Available from: https://nimedhealth.com.ng/2020/05/18/zobo-drink-hibiscus-tea-health-benefits-side-effects-nutritional-content-preparation/
Healthlink. Health Benefits of Zobo Drink, Side Effects and More. 2021. Retrieved on Dec 09 2023. Available from: https://healthlink.ng/health-benef its-of-zobo-drink/
Ilondu EM, Iloh AC. Inhibition of Three Fungal Isolates from Sorrel Drink (Zobo) Using Hurdle Technique. World Journal of Agricultural Sciences, 2007;3: 339-343.
Lin HH, Chen JH, Wang CJ. Chemopreventive Properties and Molecular Mechanisms of the Bioactive Compounds in Hibiscus sabdariffa Linne. Curr Med Chem., 2011;18:1245-1254.
Mbaeyi-Nwaoha IE, Egbuche NI. Microbiological Evaluation of Sachet Water and Street-Vended Yoghurt and “Zobo” Drinks Sold in Nsukka Metropolis. International Journal of Biological and Chemical Sciences, 2012;6:1703-1717.
Mkpuma N, Mkpuma S, Agusi ER, Okongwu-Ejike C, Atai RB, Bakare EL, Budaye J, Sule KG, Rindaps RJ, James GK, Iroha I, Eze CO. Prevalance and Antibiogram of E. coli Species Isolated from Zobo Drink Commercially available in University of Nigeria Nsukka Campus. Journal of Pharmaceutical and Allied Sciences, 2022;17:3323-3328.
Nisha R. Most Probable Number (MPN) Test: Principle, Procedure, Results. 2022. Retrieved on Dec 09 2023. Available from: https:// microbeonline.com/probable-number-mpn-test-principle-procedure-results/
Nwachukwu JP, Iredia FC, Chinasa MO. Medicinal uses and shelf life of Hibiscus sabdariffa. International Journal of Herbal Medicine, 2007;2:45-49.
Nwafor OE, Ikenebomeh MJ. Effects of different packaging materials on microbiological, physio-chemical and organoleptic quality of zobo drink storage at room temperature. African Journal of Biotechnology, 2009;8:2848-2852.
Obasi CJ, Obasi I, Okafor U, Umeh SO, Okeke O. Bacteriological Examination of Sorrel Beverage (Zobo) Drinks Sold in Awka Metropolis. Bioscience and Bioengineering, 2018;4:14-17.
Ochei JO, Kolhatkar A. Medical Laboratory Science: Theory and Practice. 2008. Tata McGraw Publishing Company Limited. Seventh Edition.
Ojo AD, Oluwalala B, Selestia M. Water Quality and Improper Hygiene in the Production of Non Alcoholic Beverage, Zobo. Journal of Food Science and Quality Management, 2011;5: 225-228.
Okeke CN, Iroka FC, Chukuruma MO. Phytochemical Analysis and Medicinal uses of Hibiscus sabdariffa. International Journal of Herbal Medicine, 2015;2:16-19.
Olayemi F, Adedayo R, Muhummad R, Bamishaiye E. The Nutritional Quality of Three Varieties of Zobo (Hibiscus sabdariffa) Subjected to the Same Preparation Condition. American Journal of Food Technology, 2011;6:705-708.
Olutiola PO, Famurewa O, Sonntag HG. Introduction to Microbiology. 2000. Second Edition, Heidelberg: Nigeria.
Omemu AM, Edema MO, Atayese AO, Obadina AO. A survey of the Microflora of Hibiscus Sabdariffa (Roselle) and the Resulting Zobo Juice. African Journal of Biotechnology, 2006;5:254-259.
Pakpour N, Horgan S. General Microbiology Lab Manual. 2021. California State University East Bay- LibreTexts. Available from: https://www.readkon g.com/page/general-microbiology-lab-manual-pakpour-horgan-5316967
Raimi OR. Bacteriology Quality of Zobo Drinks Consumed in Some Parts of Osun State, Nigeria. Journal of Applied Science and Environmental Management, 2013;17:113-117.
Ruiz-Ramírez R, Hernández-Morales J, Ayala-Escobar V, Soto-Rojas L. Fungi Associated to Calyxes of Roselle (Hibiscus sabdariffa L.) Dried and Stored in Guerrero, México. Revista Mexicana de Fitopatología, 2015;33:12-30.
Sylvester CI, Langlcy AO, Lovet TK. A review of the quality assessment of Zobo drink consumed in Nigeria. ASIO Journal of Microbiology, Food Science and Biotechnological Innovations, 2015;1:34-43.
Udensi CG, Nwankpa UD, Amanze EK, Nwokafor CV, Chinedu E, Udekwu CE, Ndubuisi CW. Microbiological Analysis of Zobo Drink Preserved with Scent Leaves (Ocimum gratissimum). South Asian Journal of Research in Microbiology, 2020;8:1-10.
World Health Organization (WHO). A global overview of national regulations and standards for drinking water quality. 2nd Edition. 2020;83. Retrieved on Dec 03 2023. Available from: https://iris.who.int/bitstream/handle/10665/35098 1/9789240023642-eng.pdf?sequence=1.
Zumbes JH, Dabo AD, Dakul DA, Afolabi SA, Dapiya MS. Enter Pathogenic Bacterial Contamination of Some Ready to Eat Foods Sold in Jos Metropolis, Nigeria. Indian Journal of Applied Research, 2014;4:456-458.
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Copyright (c) 2024 ADENIYI OLAREWAJU ADELEYE, Tolulope Deborah Akinterinwa, Florence Kemi Amoo, Afeez Oladeji Amoo, Catherine Iyabo Asaju, Peter Gimba Shiaka, Mohammed Bello Yerima
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