DISTRIBUTION OF VIRULENCE FACTORS AMONG VANCOMYCIN RESISTANT ENTEROCOCCUS FAECALIS FROM DENTAL ISOLATES

Authors

  • Prasanth M VIT University
  • Gowtham Kumar G
  • Hajarah Hussain
  • Benedict Paul C

Abstract

ABSTRACT
Objective: Enterococcus faecalis causing serious infections especially as a nosocomial pathogen was reinforced in many epidemiological studies. Many
virulence factors were found to be involved in the pathogenesis of enterococcal infections and understanding of those factors are still limited. The aim
of this study was to detect the presence of seven virulence genes in E. faecalis isolates from various dental conditions.
Methods: A total of 42 E. faecalis isolates that were found to be vancomycin resistant were studied. Identification of the isolates was done by
biochemical methods and 16s rRNA and screened for the presence of virulence genes eep, ace, asa1, asa373, enlA, fsr, and sprE using PCR.
Results: All the 42 isolates were found to contain at least one and concomitantly up to as many as six virulence genes, with three or four being a
common pattern. Most of the strains carried the ace gene (95%), and other genes were present at the frequency of 33% to 90% as well and 12% of
the isolates carried eep+ace+asa1+asa373+fsr+sprE pattern in combination.
Conclusion: From the data, it was observed that with different dental (clinical) conditions both dental caries and gingivitis were found to have various
and highest prevalence of virulence factors though all the virulence genes were observed randomly in all the isolates. It should be pointed out that
gene silencing could play its part in virulence determinants regardless of mere presence of virulence gene.
Keywords: Virulence factors, Aggregation substance, Enterolysin, Collagen-binding protein, Molecular detection.

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References

REFERENCES

Bhalla A, Pultz NJ, Gries DM, Ray AJ, Eckstein EC, Aron DC, et al.

Acquisition of nosocomial pathogens on hands after contact with

environmental surfaces near hospitalized patients. Infect Control Hosp

Epidemiol 2004;25(2):164-7.

Kayaoglu G, Ørstavik D. Virulence factors of Enterococcus faecalis:

Relationship to endodontic disease. Crit Rev Oral Biol Med

;15(5):308-20.

Creti R, Imperi M, Bertuccini L, Fabretti F, Orefici G, Di Rosa R,

et al. Survey for virulence determinants among Enterococcus faecalis

isolated from different sources. J Med Microbiol 2004;53(1):13-20.

Nallapareddy SR, Singh KV, Duh RW, Weinstock GM, Murray BE.

Diversity of ace, a gene encoding a microbial surface component

recognizing adhesive matrix molecules, from different strains of

Enterococcus faecalis and evidence for production of ace during human

infections. Infect Immun 2000;68(9):5210-7.

Johnson JR, Clabots C, Hirt H, Waters C, Dunny G. Enterococcal

aggregation substance and binding substance are not major contributors

to urinary tract colonization by Enterococcus faecalis in a mouse

model of ascending unobstructed urinary tract infection. Infect Immun

;72:2445-8.

Nakayama J, Kariyama R, Kumon H. Description of a 23.9-kilobase

chromosomal deletion containing a region encoding fsr genes which

mainly determines the gelatinase-negative phenotype of clinical

isolates of Enterococcus faecalis in urine. Appl Environ Microbiol

;68:3152-5.

Pinkston KL, Gao P, Diaz-Garcia D, Sillanpää J, Nallapareddy SR,

Murray BE, et al. The Fsr quorum-sensing system of Enterococcus

faecalis modulates surface display of the collagen-binding MSCRAMM

Ace through regulation of gelE. J Bacteriol 2011;193(17):4317-25.

Sedgley CM, Nagel AC, Shelburne CE, Clewell DB, Appelbe O,

Molander A. Quantitative real-time PCR detection of oral Enterococcus

faecalis in humans. Arch Oral Biol 2005;50:575-83.

Dahlen G, Blomqvist S, Almstahl A, Carlen A. Virulence factors and

antibiotic susceptibility in Enterococci isolated from oral mucosal and

deep infections. J Oral Microbiol 2012;4.

Lins RX, de Oliveira Andrade A, Hirata Junior R, Wilson MJ,

Lewis MA, Williams DW, et al. Antimicrobial resistance and virulence

traits of Enterococcus faecalis from primary endodontic infections.

J Dent 2013;41(9):779-86.

Penas PP, Mayer MP, Gomes BP, Endo M, Pignatari AC, Bauab KC,

et al. Analysis of genetic lineages and their correlation with virulence

genes in Enterococcus faecalis clinical isolates from root canal and

systemic infections. J Endod 2013;39(7):858-64.

Zoletti GO, Pereira EM, Schuenck RP, Teixeira LM, Siqueira JF Jr, dos

Santos KR. Characterization of virulence factors and clonal diversity

of Enterococcus faecalis isolates from treated dental root canals. Res

Microbiol 2011;162(2):151-8.

Preethee T, Kandaswamy D, Hannah R. Molecular identification

of an Enterococcus faecalis endocarditis antigen efaA in root

canals of therapy-resistant endodontic infections. J Conserv Dent

;15(4):319-22.

Zhu X, Wang Q, Zhang C, Cheung GS, Shen Y. Prevalence, phenotype,

and genotype of Enterococcus faecalis isolated from saliva and

root canals in patients with persistent apical periodontitis. J Endod

;36(12):1950-5.

Salah R, Dar-Odeh N, Abu Hammad O, Shehabi AA. Prevalence

of putative virulence factors and antimicrobial susceptibility of

Enterococcus faecalis isolates from patients with dental Diseases. BMC

Oral Health 2008;8:17.

Bittencourt de Marques E, Suzart S. Occurrence of virulence-associated

genes in clinical Enterococcus faecalis strains isolated in Londrina,

Brazil. J Med Microbiol 2004;53:1069-73.

Pillai SK, Sakoulas G, Gold HS, Wennersten C, Eliopoulos GM,

Moellering RC Jr, et al. Prevalence of the fsr locus in Enterococcus

faecalis infections. J Clin Microbiol 2002;40(7):2651-2.

Nallapareddy SR, Qin X, Weinstock GM, Höök M, Murray BE.

Enterococcus faecalis adhesin, ace, mediates attachment to extracellular

matrix proteins collagen type IV and laminin as well as collagen type I.

Infect Immun 2000;68(9):5218-24.

Hancock HH rd, Sigurdsson A, Trope M, Moiseiwitsch J. Bacteria

isolated after unsuccessful endodontic treatment in a North American

population. Oral Surg Oral Med Oral Pathol Oral Radiol Endod

;91(5):579-86.

Sjögren U, Figdor D, Persson S, Sundqvist G. Influence of infection at

the time of root filling on the outcome of endodontic treatment of teeth

with apical periodontitis. Int Endod J 1997;30(5):297-306.

Peciuliene V, Balciuniene I, Eriksen HM, Haapasalo M. Isolation of

Enterococcus faecalis in previously root-filled canals in a Lithuanian

population. J Endod 2000;26(10):593-5.

Dahlén G, Samuelsson W, Molander A, Reit C. Identification and

antimicrobial susceptibility of enterococci isolated from the root canal.

Oral Microbiol Immunol 2000;15(5):309-12.

Frank KL, Vergidis P, Brinkman CL, Greenwood Quaintance KE,

Barnes AM, et al. Evaluation of the Enterococcus faecalis biofilmassociated

virulence

factors

AhrC

and Eep in rat foreign body

osteomyelitis

and

in

vitro

biofilm-Associated

antimicrobial

resistance.

PLoS

One 2015;10(6):e0130187.

Varahan S, Iyer VS, Moore WT, Hancock LE. Eep confers

lysozyme resistance to Enterococcus faecalis via the activation

of the extracytoplasmic function sigma factor Sig V. J Bacteriol

;195(14):3125-34.

Olawale KO, Fadiora SO, Taiwo SS. Prevalence of hospital-acquired

enterococci infections in two primary-care hospitals in Osogbo, South

Western Nigeria. Afr J Infect Dis 2011;5(2):40-6.

Published

01-05-2016

How to Cite

M, P., G. Kumar G, H. Hussain, and B. Paul C. “DISTRIBUTION OF VIRULENCE FACTORS AMONG VANCOMYCIN RESISTANT ENTEROCOCCUS FAECALIS FROM DENTAL ISOLATES”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 3, May 2016, pp. 103-5, https://mail.innovareacademics.in/journals/index.php/ajpcr/article/view/10327.

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