DISTRIBUTION OF VIRULENCE FACTORS AMONG VANCOMYCIN RESISTANT ENTEROCOCCUS FAECALIS FROM DENTAL ISOLATES
Abstract
ABSTRACT
Objective: Enterococcus faecalis causing serious infections especially as a nosocomial pathogen was reinforced in many epidemiological studies. Many
virulence factors were found to be involved in the pathogenesis of enterococcal infections and understanding of those factors are still limited. The aim
of this study was to detect the presence of seven virulence genes in E. faecalis isolates from various dental conditions.
Methods: A total of 42 E. faecalis isolates that were found to be vancomycin resistant were studied. Identification of the isolates was done by
biochemical methods and 16s rRNA and screened for the presence of virulence genes eep, ace, asa1, asa373, enlA, fsr, and sprE using PCR.
Results: All the 42 isolates were found to contain at least one and concomitantly up to as many as six virulence genes, with three or four being a
common pattern. Most of the strains carried the ace gene (95%), and other genes were present at the frequency of 33% to 90% as well and 12% of
the isolates carried eep+ace+asa1+asa373+fsr+sprE pattern in combination.
Conclusion: From the data, it was observed that with different dental (clinical) conditions both dental caries and gingivitis were found to have various
and highest prevalence of virulence factors though all the virulence genes were observed randomly in all the isolates. It should be pointed out that
gene silencing could play its part in virulence determinants regardless of mere presence of virulence gene.
Keywords: Virulence factors, Aggregation substance, Enterolysin, Collagen-binding protein, Molecular detection.
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