INVESTIGATION AND EFFICACY ON PROTEIN HYDROLYSATE OF PILA GLOBOSA (A FRESH WATER MOLLUSCS) IN VARIOUS BIOMEDICAL ASPECTS
Abstract
Objective: The present study is to investigate the antibacterial, antioxidant, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of
protein hydrolysate of fresh water Molluscs Pila globosa (Swainson, 1822).
Methods: Protein hydrolysate was prepared from a tissue of P. globosa by enzymatic hydrolysis. Enzyme digestion was carried out with the
enzyme trypsin. The protein concentration was estimated by Bradford's method, and the protein quantification was done by sodium dodecyl
sulfate-polyacrylamide gel electrophoresis analysis. Antibacterial assay was carried against four bacterial pathogens by well diffusion method.
Minimum inhibitory concentration (MIC) was assayed by the microdilution method against bacteria. The percentage of free radical scavenging activity
was done by DPPH method. The total antioxidant activity also carried out by phosphomolybdenum method.
Results: Digested protein hydrolysate contains 2.2 mg/ml protein concentration, obtained by Bradford's method. The molecular weight of protein
hydrolysate was obtained ranging from 13.5 to 96.9 kDa. The maximum zone was observed against Salmonella typhi 16±1 mm at 1000 µg/ml. In
MIC, activity was found to be maximum at 100 µl concentration of protein hydrolysate. The percentage of total antioxidant activity was increased
by increasing the concentration of protein hydrolysate. Maximum of 37% radical scavenging activity was found. The Fourier transform infrared
spectroscopy analysis contains peaks ranging from of 579 to 3396/cm.
Conclusion: This finding suggests the possibility that the protein hydrolysate of P. globosa may have a potential use for a therapeutic agent like
antibiotics.
Keywords: Pila globosa, Protein hydrolysate, Antibacterial activity, Total antioxidant activity, Free radical scavenging activity.
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