IN-VITRO-ANTIDIABETIC ACTIVITY OF N-BUTANOL EXTRACT OF SESAMUM INDICUM
Abstract
ABSTRACT
Objective: The present study was aimed to investigate the inhibitory effect of n-Butanol extract of black sesame seeds on the enzymes α- amylase and
α- glucosidase followed by its Total antioxidant capacity.
Methods: The Black sesame seeds were extracted in n-Butanol, powdered and stored for future studies. Phytochemical analysis was performed to
detect the presence of many phytochemicals. The extracts were screened Alpha-amylase inhibition assay and Alpha-glucosidase inhibition assay. The
total antioxidant capacity of the n-Butanolic extract was evaluated by the phosphomolybdenum method.
Results: The obtained results showed the high alpha-glucosidase inhibitory affect than the alpha-amylase inhibition when compared to standard
acarbose. Even the Total antioxidant capacity was equivalent when compared with the standard Ascorbic acid values.
Conclusions: Based on the results, it can be concluded that Sesamum indicum herb has potential phyochemicals which can be used to reduce the
postprandial hyperglycemia by inhibiting carbohydrates metabolizing enzymes α- amylase and α- glucosidase, and also to combat the free radicals by
its anti-oxidant activity.
Keywords: Black sesame seeds, n-butanol, Alpha-amylase inhibition assay, Alpha-glucosidase inhibition assay, Phosphomolybdenum reduction.
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