IN-VITRO-ANTIDIABETIC ACTIVITY OF N-BUTANOL EXTRACT OF SESAMUM INDICUM

Authors

  • Amutha K
  • Godavari A Research Scholar, Department of Biotechnology, Vels University,

Abstract

ABSTRACT
Objective: The present study was aimed to investigate the inhibitory effect of n-Butanol extract of black sesame seeds on the enzymes α- amylase and
α- glucosidase followed by its Total antioxidant capacity.
Methods: The Black sesame seeds were extracted in n-Butanol, powdered and stored for future studies. Phytochemical analysis was performed to
detect the presence of many phytochemicals. The extracts were screened Alpha-amylase inhibition assay and Alpha-glucosidase inhibition assay. The
total antioxidant capacity of the n-Butanolic extract was evaluated by the phosphomolybdenum method.
Results: The obtained results showed the high alpha-glucosidase inhibitory affect than the alpha-amylase inhibition when compared to standard
acarbose. Even the Total antioxidant capacity was equivalent when compared with the standard Ascorbic acid values.
Conclusions: Based on the results, it can be concluded that Sesamum indicum herb has potential phyochemicals which can be used to reduce the
postprandial hyperglycemia by inhibiting carbohydrates metabolizing enzymes α- amylase and α- glucosidase, and also to combat the free radicals by
its anti-oxidant activity.
Keywords: Black sesame seeds, n-butanol, Alpha-amylase inhibition assay, Alpha-glucosidase inhibition assay, Phosphomolybdenum reduction.

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Author Biography

Godavari A, Research Scholar, Department of Biotechnology, Vels University,

Research Scholar,
Department of Biotechnology,

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Published

01-07-2016

How to Cite

K, A., and G. A. “IN-VITRO-ANTIDIABETIC ACTIVITY OF N-BUTANOL EXTRACT OF SESAMUM INDICUM”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 4, July 2016, pp. 60-62, https://mail.innovareacademics.in/journals/index.php/ajpcr/article/view/8700.

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Original Article(s)