OCIMUM BASILICUM L. ESSENTIAL OIL COATED BIOMATERIAL SURFACES PREVENT BACTERIAL ADHESION AND BIOFILM GROWTH
Abstract
Objective: Biomaterials associated infection is the most common issue associated with the biomaterial implants regardless of its form or function.
Bacteria form colonies and this result in the formation of biofilm on the surface, making the infection unreceptive to antibiotics and host defense
mechanisms. The implant is removed as an outcome. Medicinal plants have widespread usage for their active biomolecules, and the study of their
antimicrobial activities has gained widespread importance.
Methods: In this study, the essential oil of Ocimum basilicum L. (OB) is coated on biomaterial surfaces to study their efficacy in preventing bacterial
colonization and biofilm formation. The essential oil is coated on polymethylmethacrylate and polystyrene substratum surfaces. Gram-positive
bacteria, including Staphylococcus aureus and Staphylococcus epidermidis, and Gram-negative bacteria, including Escherichia coli and Pseudomonas
aeruginosa, are allowed to adhere and grow for 1 hr, 3 hrs, and 24 hrs.
Results: The number of bacteria adhering to the coated surfaces is significantly less (**p<0.01) compared to uncoated surfaces, at the measured
instances of time. The zone of inhibition of the essential oil is observed for both Gram-positive and Gram-negative bacteria. Maximum inhibition was
observed for S. aureus (30±1.2 mm diameter) compared to S. epidermidis (28±0.8 mm diameter), E. coli (25±1.1 mm diameter), and P. aeruginosa
(21±0.6 mm diameter).
Conclusion: The study reveals potent bacteriostatic effects of OB essential oil on both Gram-positive and Gram-negative bacteria. Thus, OB. essential
oil serves to be a promising coating on the implant surfaces for preventing bacterial adhesion and biofilm growth.
Keywords: Biomaterials, Bacterial adhesion, Biofilm, Ocimum bacilicum L., Antibacterial coating.
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References
Davis N, Curry A, Gambhir AK, Panigrahi H, Walker CR, Wilkins EG,
et al. Intraoperative bacterial contamination in operations for joint
replacement. J Bone Joint Surg Br 1999;81(5):886-9.
Hughes SP, Anderson FM. Infection in the operating room. J Bone Joint
Surg Br 1999;81(5):754-5.
Khalil H, Williams RJ, Stenbeck G, Henderson B, Meghji S, Nair SP.
Invasion of bone cells by Staphylococcus epidermidis. Microbes Infect
;9(4):460-5.
Broekhuizen CA, de Boer L, Schipper K, Jones CD, Quadir S,
Feldman RG, et al. Peri-implant tissue is an important niche for
Staphylococcus epidermidis in experimental biomaterial-associated
infection in mice. Infect Immun 2007;75(3):1129-36.
Vuong C, Otto M. Staphylococcus epidermidis infections. Microbes
Infect 2002;4(4):481-9.
Gristina AG. Biomaterial-centered infection: Microbial adhesion versus
tissue integration. Science 1987;237(4822):1588-95.
Anderson JM. Inflammation, wound healing, and the foreign-body
response. In: Ratner BD, Hoffman AS, Schoen FJ, Lemons JE, editors.
Biomaterials Science. An Introduction to Materials in Medicine. San
Diego, CA: Elsevier; 2004. p. 296-304.
Xia Z, Triffitt JT. A review on macrophage responses to biomaterials.
Biomed Mater 2006;1(1):R1-9.
Berton G, Lowell CA. Integrin signalling in neutrophils and
macrophages. Cell Signal 1999;11(9):621-35.
Maddikeri RR, Tosatti S, Schuler M, Chessari S, Textor M, Richards RG,
et al. Reduced medical infection related bacterial strains adhesion on
bioactive RGD modified titanium surfaces: A first step toward cell
selective surfaces. J Biomed Mater Res A 2008;84(2):425-35.
Subbiahdoss G, Grijpma DW, van der Mei HC, Busscher HJ, Kuijer R.
Microbial biofilm growth versus tissue integration on biomaterials with
different wettabilities and a polymer-brush coating. J Biomed Mater
Res A 2010;94(2):533-8.
Fitzgerald RH Jr. Microbiologic environment of the conventional
operating room. Arch Surg 1979;114(7):772-5.
Verkkala K, Eklund A, Ojajärvi J, Tiittanen L, Hoborn J, Mäkelä P.
The conventionally ventilated operating theatre and air contamination
control during cardiac surgery - Bacteriological and particulate matter
control garment options for low level contamination. Eur J Cardiothorac
Surg 1998;14(2):206-10.
Dougherty SH. Pathobiology of infection in prosthetic devices. Rev
Infect Dis 1988;10(6):1102-17.
Boelens JJ, Dankert J, Murk JL, Weening JJ, van der Poll T,
Dingemans KP, et al. Biomaterial-associated persistence of
Staphylococcus epidermidis in pericatheter macrophages. J Infect Dis
;181:1337-49.
Massey RC, Horsburgh MJ, Lina G, Höök M, Recker M. The evolution
and maintenance of virulence in Staphylococcus aureus: A role for hostto-host
transmission? Nat Rev Microbiol 2006;4(12):953-8.
Babior BM. Oxidants from phagocytes: Agents of defense and
destruction. Blood 1984;64(5):959-66.
Günther F, Wabnitz GH, Stroh P, Prior B, Obst U, Samstag Y, et al. Host
defence against Staphylococcus aureus biofilms infection: Phagocytosis
of biofilms by polymorphonuclear neutrophils (PMN). Mol Immunol
;46(8-9):1805-13.
Valtonen MV, Plosila M, Valtonen VV, Mäkelä PH. Effect of the quality
of the lipopolysaccharide on mouse virulence of Salmonella enteritidis.
Infect Immun 1975;12(4):828-32.
Valtonen MV. Role of phagocytosis in mouse virulence of Salmonella
typhimurium recombinants with O antigen 6,7 or 4,12. Infect Immun
;18(3):574-82.
Bonventre PF, Imhoff JG. Uptake of h-dihydrostreptomycin by
macrophages in culture. Infect Immun 1970;2(1):89-95.
Tada H, Murakami Y, Omoto T, Shimomura K, Ishimaru K. Rosmarinic
acid and related phenolics in hairy root cultures of Ocimum basilicum.
Phytochemistry 1996;42(2):431-4.
Reuveni R, Raviv M, Krasnovsky A, Freiman L, Medina S, Bar A,
et al. Compost induces protection against Fusariumoxysporum in sweet
basil. Crop Protection 2002;21:583-7.
Chiej R. Encyclopaedia of Medicinal Plants. London: MacDonald;
Lust J. The Herb Book. New York: Bantam Books; 1983.
Duke JA, Ayensu ES. Medicinal Plants of China. Algonac, MI:
Reference Publications, Inc.; 1985.
Chopra RN, Nayar SL, Chopra IC. Glossary of Indian Medicinal Plants
(Including the Supplement). New Delhi: Council of Scientific and
Industrial Research; 1986.
Martin KW, Ernst E. Herbal medicines for treatment of fungal
infections: A systematic review of controlled clinical trials. Mycoses
;47(3-4):87-92.
Davies J. Inactivation of antibiotics and the dissemination of resistance
genes. Science 1994;264(5157):375-82.
Loper JE, Henkels MD, Roberts RG, Grove GG, Willet MJ, Smith TJ.
Evaluation of streptomycin, oxytetracycline, and copper resistance of
Erwinia amylovora isolated from pear orchards in Washington State.
Plant Dis 1991;75(3):287-90.
Asian J Pharm Clin Res, Vol 9, Issue 3, 2016, 379-384
Rajaraman et al.
Service RF. Antibiotics that resist resistance. Science
;270(5237):724-7.
Clark AM. Natural products as a resource for new drugs. Pharm Res
;13(8):1133-44.
Cordell GA. Biodiversity and drug discovery - A symbiotic relationship.
Phytochemistry 2000;55(6):463-80.
Sivaramakrishnan R, Guruprakash S, Gnanaprakash D, Subashini R.
Ocimum basilicum extract coating on biomaterial surfaces to prevent
bacterial adhesion and biofilm growth. Asian J Pharm Clin Res
;8(3):229-33.
Adigzel A, Gulluce M, Sengul M, Ogutcu H, Sahin F, Karaman U.
Antimicrobial effects of Ocimum basilicum (Labiatae) extract. Turk J
Biol 2005;29:155-60.
Da-Silva F, Santos RHS, Diniz ER, Barbosa LC, Casali VW,
De-Lima RR. Content and composition of basil essential oil at
two different hours in the day and two seasons. Braz J Med Plants
;6(1):33-8.
Helal GA, Sarhan MM, Abu Shahla AN, Abou El-Khair EK.
Antimicrobial activity of some essential oils against microorganisms
deteriorating fruit juices. Mycobiology 2006;34(4):219-29.
Runyoro D, Ngassapa O, Vagionas K, Aligiannis N, Graikou K,
Chinou I. Chemical composition and antimicrobial activity of the
essential oils of four Ocimum species growing in Tanzania. Food Chem
;119(1):311-6.
Gutierrez J, Barry-Ryan C, Bourke P. The antimicrobial efficacy of
plant essential oil combinations and interactions with food ingredients.
Int J Food Microbiol 2008 10;124(1):91-7.
Hussain AI, Anwar F, Hussain Sherazi ST, Przybylski R. Chemical
composition, antioxidant and antimicrobial activities of basil (Ocimum
basilicum) essential oils depends on seasonal variations. Food Chem
1;108(3):986-95.
Ravid U, Putievsky E, Katzir I, Lewinsohn E. Enantiomeric composition
of linalool in the essential oils of Ocimum species and in commercial
basil oils. Flavour Fragr J 1997;12(4):293-6.
Mazzanti G, Battinelli L, Salvatore G. Antimicrobial properties of the
linalool-rich essential oil of Hyssopus officinalis L. Vardecumbens
(Lamiaceae). Flavour Fragr J 1998;13:289-94.
Politeo O, Jukic M, Milos M. Chemical composition and antioxidant
capacity of free volatile aglycones from basil (Ocimum basilicum L.)
compared with its essential oil. Food Chem 2007;101:379-85.
Prasad G, Kumar A, Singh AK, Bhattacharya AK, Singh K, Sharma VD.
Antimicrobial activity of essential oils of some Ocimum species and
clove oil. Fitoterapia 1986;57:429-32.
Opalchenova G, Obreshkova D. Comparative studies on the activity of
basil – An essential oil from Ocimum basilicum L. – Against multidrug
resistant clinical isolates of the genera Staphylococcus, Enterococcus
and Pseudomonas by using different test methods. J Microbiol Methods
;54(1):105-10.
Sinha GK, Gulati BC. Antibacterial and antifungal study of
some essential oils and some of their constituents. Indian Perfum
;34:126-9.
Zheljazkov VD, Cantrell CL, Tekwani B, Khan SI. Content,
composition, and bioactivity of the essential oils of three basil
genotypes as a function of harvesting. J Agric Food Chem
;56(2):380-5.
Nazzaro F, Fratianni F, De Martino L, Coppola R, De Feo V. Effect
of essential oils on pathogenic bacteria. Pharmaceuticals (Basel)
;6(12):1451-74.
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