ANTICANCER STUDY OF MYRISTICA FRAGRANS HOUTT. (MACE) EXTRACT ON 4-NITROQUINOLINE-1-OXIDE-INDUCED ORAL CANCER IN RATS

Gayathri R; Anuradha Venkataraman; Vishnupriya V; Mallika Jainu

doi:10.22159/ajpcr.2018.v11i7.25363

Authors

Gayathri R Department of Biochemistry, Research and Development Centre, Bharathiyar University, Coimbatore - 641 106, Tamil Nadu, India.
Anuradha Venkataraman Department of Biochemistry, Mohammed Sathak College of Arts and Science, Sholinganallur, Chennai - 600 119, Tamil Nadu, India.
Vishnupriya V Department of Biochemistry, Saveetha Dental College and Hospital, Saveetha Institute of Medical & Technical Sciences, Saveetha University, Velappanchavadi, Chennai - 600 077, Tamil Nadu, India.
Mallika Jainu Department of Biochemistry, Biogen Care Research Center, Chennai - 600 014, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i7.25363

Keywords:

Oral cancer, 4-nitroquinoline-1-oxide, Myristica fragrans Houtt, Histopathology

Abstract

Objective: Oral cancer is the most common forms of malignancy diagnosed in men and women and the mortality rate is very high in the developing countries. Therefore, the study was conducted to evaluate the potential role of Myristica fragrans Houtt (mace) acetone extract (MAE) on experimentally induced oral cancer in rats.

Methods: Oral cancer was experimentally induced to rats by administering 4NQO (carcinogenic agent) at a concentration of 30 ppm in drinking water and treated with three different doses of mace extract (100, 250, 500mg/kg body weight) dissolved in water and given orally as a single dose and another group treated with the standard 5-fluorouracil for 14 weeks.

Results: All the experimental groups had an incremental weight gain, except NQO alone induced group showed body weight loss and also showed low survival rate denoting the NQO carcinogenic toxicity in them. The exophytic tumor volume showed gradual increase and reached 30 mm3 at the 14 weeks time point. Tumor incidence and tumor multiplicity values of mace extract treatment (100, 250, 500 mg/kg body weight) showed gradual reduction in the size of tumor significantly compared with that of the standard drug. The histopathological examination of oral tumor mucosal tissue illustrated well differentiated squamous cell carcinoma in NQO induced group and also declining the risk of carcinoma changes in MAE treated groups.

Conclusions: These experimental results unveil that MAE possesses effective role in curing oral cancer.

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