PHYTOCHEMICAL COMPOSITION AND ANTICANCER ACTIVITY OF SEAWEEDS ULVA LACTUCA AND EUCHEUMA COTTONII AGAINST BREAST MCF-7 AND COLON HCT-116 CELLS
DOI:
https://doi.org/10.22159/ajpcr.2016.v9i6.13798Abstract
Objective: this study is aimed to develop marine resources which is focused on the determination of  phytochemical composition and exploration of seaweeds Ulva Lactuca and Eucheuma cotonii, as a potential anti-breast cancer and anti-colorectal cancer agents.
Â
Methods: Seaweeds Ulva Lactuca collected from Parangtritis beach, Yogyakarta, Central Java, Indonesia. Whereas Eucheuma cottonii collected from Salemo island, South Sulawesi, Indonesia. Seaweeds U. lactuca and E. cottonii were macerated in organic solvents, n-hexane, chloroform, ethyl acetate and ethanol, respectively. After maceration for three days, the mixture was filtered, the filtrate was concentrated by rotary evaporator. The concentrated extract of n-hexane, ethyl acetate, ethanol and chloroform were then analyzed by thin layer chromatography. Phytochemical test of the concentrated extract were conducted to identify the metabolites containing in the seaweeds. Furthermore, the cytotoxic activity of the n-hexane, ethyl acetate, ethanol and chloroform extract of Ulva Lactuca and Eucheuma cotonii were evaluated as a growth inhibitor of breast MCF-7 and colorectal HCT-116 cancer cells by MTT cell proliferation assay.
Â
Results: Phytochemical test for the  concentrated extracts of Ulva Lactuca showed the positive result for metabolites of  steroids, glycosides, flavonoid,and tannin. While  the concentrated extracts of E. cottonii showed positive result for metabolites of steroids, glycosides, and flavonoid. Both concentrated extracts of Ulva lactuca and Eucheuma cotonii exhibited anticancer activity against breast MCF-7 and colorectal HCT-116 cells with IC50 ranging of 21 µg/mL to 99 µg/mL .
Â
Conclusion: Our results clearly demonstrate seaweeds Ulva Lactuca and Eucheuma cotonii as a promising candidates for the new anti-breast and anti-colorectal cancer agents.
Â
Keywords: Phytochemistry, Ulva Lactuca, Eucheuma cotonii , anticancer, breast MCF-7, colorectal HCT-116
Downloads
References
IARC. World Cancer Report. Geneva: International Agency for Research on Cancer (IARC), 2012.
Ministry of Health Republic of Indonesia; 2015. Available from: http://www.depkes.go.id/article/view/201407070001/hilangkan-mitos-tentang-kanker.html.
Aziz MF, Andrijono A, Saifuddin AB. Onkologi dan Ginekologi: Buku Acuan Nasional. Jakarta, Indonesia: Yayasan Bina Pustaka Sarwono Prawirohardjo; 2006. p. 442-55.
Lage, H. Drug resistance in breast cancer. Cancer Ther 2003;1:81-91.
Zheng Y, Zhou J, Tong Y. Gene signature of drug resistance predict patient survival in colorectal cancer. Pharmacogenomics J 2014;20:1-9.
Arsianti A, Tanimoto H, Morimoto T, Bahtiar A, Takeya T, Kakiuchi K. Synthesis and anticancer activity of polyhydroxylated 18 - Membered analogue of antimycin A3. Tetrahedron 2012;68(13):2884-91.
Arsianti A, Fadilah F, Kusmardi K, Tanimoto H, Morimoto, T, Kakiuchi K. Design and molecular docking study of antimycin A3 analogues as inhibitors of anti-apoptotic Bcl-2 of breast cancer. Open J Med Chem 2014;4(3):79-86.
Arsianti A, Fadilah F, Kusmardi K, Tanimoto H, Kakiuchi K. Design, synthesis and cytotoxicity evaluation of novel open-chain analogues of antimycin A3 as potential anti colorectal cancer agents. AJPCR 2015;8(6):120-4.
Tempo News: Jokowi Yakin Indonesia Jadi Poros Maritim Dunia. Available from: http://www.tempo.co/read/news/2014/11/13/118621693/Jokowi-Yakin-Indonesia-Jadi-Poros-Maritim-Dunia.
Sipkema D, Franssen MC, Osinga R, Tramper J, Wijffels RH. Marine sponges as pharmacy. Mar Biotechnol (NY) 2005;7(3):142-62.
Mayer AM, Rodriguez AD, Berlinck RG, Hamman MT. Marine pharmacology in 2005-2006: Marine compounds with anthelmintic, antibacterial, anticoagulant, antifungal, anti-inflamatory, antimalarial, antiprotozoal, antituberculosis, and antiviral activities; Affecting the cardiovascular, Immune and nervous system, and other miscellaneous mechanisms of action. Biochim Biophys Acta 2009;1790(5):283-308.
Kim EJ, Park SY, Lee JY, Park JH. Fucoidan present in brown algae induces apoptosis of human colon cancer cells. BMC Gastroenterol 2010;10:96.
Lee JC, Hou MF, Huang HW, Chang FR, Yeh CC, Tang JY, et al. Marine algal natural products with anti-oxidative, anti-inflammatory, and anti-cancer properties. Cancer Cell Int 2013;13(1):55.
Michanek G. Seaweed resources for pharmaceutical uses. In: Marine Algae in Pharmaceutical Science. Berlin, New York: Walter de Gruyter; 1979.
Noda H, Amano H, Arashima K, Nizizawa K. Antitumor activity of marine algae. Hydrobiologia 1990;204:577-84.
Aslan LM. Budidaya Rumput Laut. Yogyakarta: Kanisius; 1998.
Luning K. Seaweed: Their Environment, Biogeography and Ecophysiology. New York: John Wiley and Sons; 1990.
Atmadja WS, Kadi A, Sulistidjo S, Rachmaniar R. Pengenalan Jenis-Jenis Rumput Laut. Jakarta, Indonesia: Pusat Penelitian dan Pengembangan Oseanologi LIPI; 1996.
Bayu A. Biogas production from macroalgae Ulva lactuca and Padina sp. As energy resources. Oseanology and Limnology in Indonesia 2012;38(3):401-11.
Bruhn A, Dahl A, Nielsen HB, Nikolaisen L, Markager S, Olesen B, et al. Bioenergy potential of Ulva lactuca, biomass yield, methane production and combustion. Bioresour Technol 2016;102(3):2595-604.
Kolar J, Machackova I. Occurrence and possible function of melatonin in plants. Endpcytosis Cells Res 2001;14(1):75-84.
Ciulei I. Methodology for analysis of vegetable drugs, practical manuals on industrial utilization of medicinal and aromatic plant. Phytochemistry 1982;63(1):97-104.
Harborne JB. Metode Fitokimia. Bandung, Indonesia: ITB; 1987.
Cook NC, Samman S. Review flavonoids-chemistry metabolism, cardioprotective effect, and dietary sources. J Nutr Biochem 1996;(7):66-76.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
