THE ANTIBACTERIAL EVALUATION OF HALICLONA ASSOCIATED BACTERIA AND THE RELATING COMPOUNDS DERIVED FROM THE HOST
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i7.25510Keywords:
Antibacterial, Active compounds, Haliclona (gellius) sp, Agromyces tropicusAbstract
Objective: The objective of this study was to investigate the active compounds derived from Haliclona sp. associated bacteria collected from Untung Jawa Island, Jakarta.
Methods: The bacterial isolation, screening of antibacterial activity, purification, and prediction of potential compounds using liquid chromatography– mass spectroscopy/mass spectroscopy (LC–MS/MS) as well as 16S rDNA bacterial characterization were conducted.
Results: The potential extract of bacterial strain UJ.17.10-4 showed the highest anti-Gram-positive bacteria. The 16S rDNA gene of this strain had 99% similarity with the actinobacteria Agromyces tropicus. Chemical separation of supernatant extract yielded 13 potent fractions. Identification of antibacterial compounds contained in active fractions using LC-MS/MS were halistanol sulphate C([M+H]+:703), cyclic bis-1,3- dialkylpyridinium([M+H]+: 213.72). In previous research reported that these compounds were isolated from Haliclona sponge and showed anticancer activity.
Conclusion: This result supported the ideas that A. tropicus plays an important role in synthesizing, halistanol sulfate C, haliclorensin, and cyclic bis- 1,3-dialkylpyridinium metabolite derived from the host.
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