ANTIHYPERURICEMIC AND XANTHINE OXIDASE INHIBITORY ACTIVITIES OF FRACTIONS FROM ETHANOLIC LEAVES EXTRACT OF STELECHOCARPUS BURAHOL
DOI:
https://doi.org/10.22159/ajpcr.2016.v9i6.14314Abstract
Objectives: The aims of the research were to evaluate antihyperuricemic and xanthine oxidase (XO) inhibitory activity of fractions from ethanolic leaves extract of Stelechocarpus burahol.
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Methods: S. burahol leaves powder was extracted in ethanol by maceration method, then extract was fractionated by liquid-liquid extraction method using n-hexane and ethyl acetate. Hyperuricemic rat model was induced by administered potassium oxonate intraperitoneally. The activity of these fractions on XO inhibitory was determined by measuring the uric acid formation using UV-Vis spectrophotometry method after incubating with XO. Â
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Results: The n-hexane and ethyl acetate fractions significantly (p < 0.05) reduce the uric level by 32.0 and 28.0% respectively compared to uric acid level of hyperuricemic group at one hour after drug administration (p < 0.05). On XO inhibitory activity test, only n-hexane fraction exhibited XO inhibitory activity with IC50 541.76 µg/ml.
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Conclusion: The n-hexane and ethyl acetate fractions from ethanolic leaves extract of S.burahol had antihiperuricemic activity, but less inhibitor effect on XO activity.
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Keywords: Stelechocarpus burahol, liquid-liquid extraction, antihyperuricemic, xanthine oxidase inhibitory activityÂ
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