MOLECULAR AUTHENTICATION AND GENETIC DIVERSITY ANALYSIS OF HALOFEROX VOLCANII AND HALOBACTERIUM SALINARIUM FROM SALT BRINES
Abstract
Objective - Halophilic bacteria present in various high salt locations have shown to possess various commercially viable properties. An understanding of the microbial flora present in salt brines will help in the proper exploitation of this resource.
Methods - In the current study, bacteria were isolated from salt brines and characterized. Followed by the morphological and biochemical characterization, the strains were subjected to molecular characterization. Genomic DNA was isolated and the 16s rRNA gene was amplified and sequenced. The sequence so obtained was subjected to sequence alignment analysis. The genetic diversity of the isolated strains were also analysed by RAPD PCR and phylogenetic relationship was established by dendrogram construction.
Results – The isolated strains were found to be Haloferax volcanii and Halobacterium salinarium. The genetic diversity analyses of H. salinarium and H. volcanii reveal the resistance of the former to genetic variations.
Conclusion - This study analysed the impact of environmental stress on the genotypes of H. salinarium and H. volcanii.
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References
DeLong, E. F.: Everything in moderation: archaea as 'non-extremophiles'. Curr. Opin. Genet. Dev., 1998; 8: 649–654.
Grant, W. D., Larsen, H., Extremely halophilic archaeobacteria. In: Staley, Bryant, Pfennig and Holt (Eds.), Bergy’s Manual of Systematic Bacteriology, 1st Ed., Vol 3, The Williams & Wilkins Co., Baltimore, 1989 ; pp. 2216-2219.
Thongthai, C., McGenity, T. J., Suntinanalert, P., Grant, W. D. : Isolation and characterization of an extremely halophilic archaeobacterium from traditionally fermented Thai fish sauce (nam pla). Lett. Appl. Microbiol., 1992; 14: 111-114.
Oren A.: Diversity of halophilic microorganisms: Environments, phylogeny, physiology, and applications. Journal of Industrial Microbiology & Biotechnology., 2002; 28, 56–63.
Elevi, R., Assa, P., Birbir, M., Ogan, A., Oren, A.: Characterization of extremely halophilic archaea isolated from the Ayvalik salterns, Turkey. World J. Microbiol. Biotechnol., 2004; 20:719-725.
Elshahed, M. S., Najar, F. Z., Roe, B. A., Oren, A., et al.,: Survey of archaeal diversity reveals an abundance of halophilic Archaea in a low-salt, sulfide- and sulfur-rich spring. Appl. Environ. Microbiol., 2004; 70, 2230-2239.
Oren, A., Ventose, A., Grant, W. D.: Proposed minimal standards for description of new taxa in the order Halobacteriales. Int J Syst Bacteriol., 1997; 47, 233-238.
Oren, A.: Life at high salt concentrations. In: Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K-H. and Stackebrandt, E. ( Eds. ) The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 3rd ed. New York: Springer – Verlag (electronic publication). 2000.
Sambrook. J., Fritsch, E. F., Maniatis, T.: Molecular cloning: a laboratory manual, 2nd edition, Cold Spring Harbour Laboratory Press, Cold spring Harbor, N.Y. 1989.
Levesque, R.: SPSS Programming and Data Management: A Guide for SPSS and SAS Users, Fourth Edition, SPSS Inc., Chicago Ill. PDF ISBN 1568273908. 2007.
Margesin, R., F. Schinner: Potential of halotolerant and halophilic microorganisms for biotechnology. Extremophiles., 2001;5, 73- 83.
Birbir M., B. Calli, B. Mertoglu, E.R. Bardavid, A. Oren et al.; Extremely halophilic archaea from Tuz Lake, Turkey, and the adjacent Kaldirim and Kayacik salterns. World J. Microbiol. Biotechnol., 2007;23: 309–316.
Oren A, Shilo M.: Bacteriorhodopsin in a bloom of halobacteria in the Dead Sea. Arch Microbiol., 1981;130: 185-187.
Burns DG, Camakaris HM, Janssen PH, Dyall-Smith ML.: Combined use of cultivation-dependent and cultivation independent methods indicate that members of most haloarchaeal groups in an Australian crystallizer pond are cultivable. Appl Environ Microbiol., 2004; 70: 5258-5265.
Power EG.: RAPD typing in microbiology--a technical review. J Hosp Infect., 1996; 34(4):247-265.
Bhathini Vaikuntavasan Pradeep, Francis Stanly Pradeep, Jayaraman Angayarkanni, Muthusamy Palaniswamy. : Optimization and production of prodigiosin from Serratia marcescens MBB05 using various natural substrates. Asian Journal of Pharmaceutical and Clinical Research. 2013; 6(1). 34-41
I.V.S. Nimal Christhudas, P. Praveen Kumar, P. Agastian.: Antimicrobial activity and HPLC analysis of tropane alkaloids in streptomyces spp. Isolated from Datura stramonium L. Asian Journal of Pharmaceutical and Clinical Research. 2012; 5(4) 278-282.
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