FLAVONOIDS AND PHENOLIC CONTENT IN WHEAT GRASS PLANT (TRITICUM AESTIVUM).
Abstract
The study was mainly designed to screen various solvent extracts of the leaves of wheatgrass (Triticum aestivum) to show the potent antioxidant
activity in order to find possible sources for novel antioxidants in food and pharmaceutical supplements. A detailed study was performed on the
antioxidant activity of the methanol extract and chloroform extract of T. aestivum. The fractions were initially screened for the phenolics and flavonoids
and then estimated for the total polyphenols and the flavonoids. Further, the fractions were used for showing the potential antioxidant activity. The
antioxidant activity was displayed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and H2O2 scavenging activity. The
plant responded positive for both the phytochemicals screened. The total phenolic content was found to be more for methanol fraction (44%) when
compared with the chloroform fraction (12%). The total flavonoids were more for the chloroform fraction (40.420 ± 0.13 μg/mL) when compared
with the methanol fraction (22.873 ± 0.05). Methanol fraction was shown to contain more polyphenols (44%), than the methanol fraction (12%).
Methanol fraction displayed strong DPPH scavenging activity (IC50 4.258 ± 0.54 minute) in reference to the positive control ascorbic acid exhibited
3.3266 ± 0.38 minute. The scavenging activities of the chloroform fraction were (IC50 5.217 ± 0.52 minute). The scavenging activity for hydrogen
peroxide of various solvent extracts from Wheatgrass was in the order of positive control > methanol > chloroform respectively. The data from H2O2
activity showed that the activity was dose-dependent. The H2O2 activity at a concentration of 200 μg/mL was found to 57.11%, 71.13%, and 86.54%
for the chloroform, methanol, and positive control fractions, respectively.
Keywords: Triticum aestivum, Total phenolics, 2,2-diphenyl-1-picrylhydrazyl, Antioxidant, H2O2 scavenging activity.
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References
Ames BN. Dietary carcinogens and anticarcinogens. Oxygen radicals
and degenerative diseases. Science 1983;221(4617):1256-64.
Bandoniene D, Murkovic M. The detection of radical scavenging
compounds in crude extract of borage (Borago officinalis L.) by
using an on-line HPLC-DPPH method. J Biochem Biophys Methods
;53(1-3):45-9.
Ben-Arye E, Goldin E, Wengrower D, Stamper A, Kohn R, Berry E.
Wheat grass juice in the treatment of active distal ulcerative colitis:
A randomized double-blind placebo-controlled trial. Scand J
Gastroenterol 2002;37(4):444-9.
Blumenthal M, editor. The Complete German Commission E
Monographs: Therapeutic Guide to Herbal Medicines. Austin, TX:
American Botanical Council; 1998.
Brand-Williams W, Cuvelier ME, Berset C. Use of free radical
method to evaluate antioxidant activity. Lebenson Wiss Technol
;28(1):25‑30.
Bursal E, Gulcin I. Polyphenol contents and in vitro antioxidant activities
of lyophilized aqueous extract of kiwifruit (Actinidia deliciosa). Food
Res Int 2011;44:1482-9.
Byers T, Nestle M, McTiernan A, Doyle C,
Currie-Williams A, Gansler T, et al. American Cancer Society
guidelines on nutrition and physical activity for cancer prevention:
Reducing the risk of cancer with healthy food choices and
physical activity. CA Cancer J Clin 2002;52(2):92-119.
Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, Vidal N.
Antioxidant activity of some Algerian medicinal plants extracts
containing phenolic compounds. Food Chem 2006;97(4):654-60.
Fattman CL, Schaefer LM, Oury TD. Extracellular superoxide dismutase
in biology and medicine. Free Radic Biol Med 2003;35(3):236-56.
Göçer H, Gülçin I. Caffeic acid phenethyl ester (CAPE): Correlationof structure and antioxidant properties. Int J Food Sci Nutr
;62(8):821‑5.
Govind P. Medicinal plants against liver diseases. Int J Pharm Res
;2(5):115-21.
Gruenwald J, Brendler T, Jaenicke C, editors. PDR for Herbal
Medicines. 3rd ed. Montvale, NJ: Thomson PDR; 2004.
Gülçin I. Antioxidant activity of food constituents: An overview. Arch
Toxicol 2012;86(3):345-91.
Halliwell B, Gutteridge JM. Formation of thiobarbituric-acid-reactive
substance from deoxyribose in the presence of iron salts: The role of
superoxide and hydroxyl radicals. FEBS Lett 1981 15;128:347-52.
Harman D. Free radical theory of aging. Current status. Amster-Dam:
Elsevier; 1998. p. 3-7.
Kasai H, Fukada S, Yamaizumi Z, Sugie S, Mori H. Action
of chlorogenic acid in vegetables and fruits as an inhibitor of
-hydroxydeoxyguanosine formation in vitro and in a rat carcinogenesis
model. Food Chem Toxicol 2000;38(5):467-71.
Lu Y, Foo LY. Identification and quantification of major polyphenols in
apple pomace. Food Chem 1997;59(2):187.
MacIntosh CJ. Wheatgrass and mold, 2008. Available from:
http://www.cityfarmer.org/wheatgrass.html. [Last accessed on Jun 10].
Maqsood S, Singh P, Samoon MH, Balange AK. Effect of dietary
chitosan on non-specific immune response and growth of Cyprinus
carpio challenged with Aeromonas hydrophila. Int Aquat Res
;2:77-85.
Nunes PX, Silva SF, Guedes RJ, Almeida S. Biological oxidations
and antioxidant activity of natural products. Phytochemicals as
Nutraceuticals - Global Approaches to Their Role in Nutrition and
Health. ???: Intech; 2012.
Oluyemi KA, Okwuonu UC, Baxter DG, Oyesola TO. Toxic effects
of methanolic exract of Aspilia africana leaf on the estrous cycle and
uterine tissues of Wistar rats. Int J Morphol 2007;25:609-614.
Omoruyi BE, Bradley G, Afolayan AJ. Antioxidant and phytochemical
properties of Carpobrotus edulis (L.) bolus leaf used for the management
of common infections in HIV/AIDS patients in Eastern Cape Province.
BMC Complement Altern Med 2012;12:215.
Ordonez AA, Gomez V, Vattuone MA, Isla MI. Antioxidant activities of
sechium edule (Jacq.) swart extracts. Food Chem 2006;97:452-8.
Osawa T, Kavakishi S, Namiki M, Kuroda Y, Shankal DM, Waters MD.
Antimutagenesis and Anticarcinogenesis Mechanisms II. New York:
Plenum; 1990. p. 139-53.
Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and
inhibition of intercellular communication by antioxidant catechins
isolated from Chinese green tea. Carcinogenesis 1989;10(6):1003-8.
Vongtau HO, Abbah J, Chindo BA, Mosugu O, Salawu AO,
Kwanashie HO, et al. Central inhibitory effects of the methanol
extract of Neorautanenia mitis root in rats and mice. J Pharm Biol
;43(2):113‑20.
Aidi Wannes W, Mhamdi B, Sriti J, Ben Jemia M, Ouchikh O,
Hamdaoui G, et al. Antioxidant activities of the essential oils and
methanol extracts from myrtle (Myrtus communis var. italica L.) leaf,
stem and flower. Food Chem Toxicol 2010;48(5):1362-70.
Zengin G, Cakmak YS, Guler GO, Aktumsek A. Antioxidant properties
of methanolic extract and fatty acid composition of Centaurea urvillei
DC. subsp. hayekiana wagenitz. Rec Nat Prod 2011;5(2):123-32.
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