SCREENING OF BIOACTIVE COMPOUNDS FROM UNKNOWN MARINE SPONGES COLLECTED FROM KOVALAM, CHENNAI

Yuvarani Thambidurai; Sudarsanam D; Habeeb Skm; Joe k Kizhakudan

doi:10.22159/ajpcr.2017.v10i5.17347

Authors

Yuvarani Thambidurai D G VAISHNAVA COLLEGE CHENNAI
Sudarsanam D LOYOLA COLLEGE CHENNAI
Habeeb Skm SRM University
Joe k Kizhakudan CMRFICHENNAI

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i5.17347

Keywords:

Dysidea herbacea, Sigmadocia pumila, Acanthella elongata, Secondary metabolites, Gas chromatography-mass spectrometry analysis, Bioactive compounds

Abstract

Objective: This study is designed to hunt for the presence of bioactive compounds from three marine sponges collected from Kovalam.

Methods: Zoochemical analysis is performed to screen for the presence of secondary metabolites. Based on those results, only two sponges which showed a significant presence of secondary metabolites has been subjected to gas chromatography-mass spectrometry (GC-MS) analysis to identify the unknown chemical compounds present in those sponges.

Results: On analyzing the results, two sponges, namely, Dysidea herbacea and Sigmadocia pumila, has shown a significant presence of secondary metabolites while the third sponge Acanthella elongata have shown moderate presence of secondary metabolites. Since the first two sponges results are remarkable, these two samples have been subjected to GC-MS analysis to separate and identify the unknown chemical compounds present in the sample.

Conclusion: Samples, namely, D. herbacea and S. pumila, indicated the presence of several components. From both the sponges, eleven different secondary metabolites were identified by GC-MS. Most of these compounds are widely used in cosmetic, pharmaceutical, and other industries and therefore a vital source for industrial biotechnology and related products in healthcare and skincare.

Â

Downloads

Download data is not yet available.

References

Proksch P, Edrada RA, Ebel R. Drugs from the seas - current status and microbiological implications. Appl Microbiol Biotechnol 2002;59(2-3):125-34.

Silinskas RA. Marine Biotechnology and the Third World: Research and Applications. Conference Proceeding Series: Harnessing Biotechnology for the 21st Century. Washington DC: American Chemical Society; 1992.

Vacelet J, Duport E. Prey capture and digestion in the carnivorous sponge Asbestopluma hypogea (Porifera: Demospongiae). Zoomorphology 2004;123(4):179.

Bhakuni DS, Rawat DS. Bioactive Marine Naturalproducts. New Delhi, India: Springer and Anamaya Publishers; 2005.

Blunt JW, Copp BR, Munro MH, Northcote PT, Prinsep MR. Marine natural products. Nat Prod Rep 2010;27(2):165-237.

Sagar S, Kaur M, Minneman KP. Antiviral lead compounds from marine sponges. Mar Drugs 2010;8(10):2619-38.

Diaz MC, Ward BB. Sponge-mediated nitrification in tropical benthic communities. Mar Ecol Prog Ser 1997;156:97-107.

Lu X, Cao X, Liu X, Jiao B. Marine microbes-derived anti-bacterial agents. Mini Rev Med Chem 2010;10(11):1077-90.

Uppala L. A review on active ingredients from marine sources used in cosmetics. SOJ Pharm Pharm Sci 2015;2(3):1-3.

Newbold RW, Jensen PR, Fenical W, Pawlik JR. Antimicrobial activity of Caribbean sponge extracts. Aquat Microb Ecol 1999;19:279-84.

Faulkner DJ. Highlights of marine natural products chemistry (1972-1999). Nat Prod Rep 2000;17(1):1-6.

Sipkema D, Franssen MC, Osinga R, Tramper J, Wijffels RH. Marine sponges as pharmacy. Mar Biotechnol (NY) 2005;7(3):142-62.

Rangel M, Sanctis B, Freitas JC, Polatto JM, Granato AC, Berlinck RG, et al. Cytotoxic and neurotoxic activities in extracts of marine sponges from southeastern Brazilian coast. J Exp Biol Ecol 2001;262:31-40.

Touati I, Chaieb K, Bakhrouf A, Gaddour K. Screening of antimicrobial activity of marine sponge extracts collected from Tunisian coast. J Med Mycol 2007;17:183-6.

Ferreira M, Cabado AG, Chapela MJ, Fajardo P, Atanassova M, Garrido A, et al. Cytotoxic activity of extracts of marine sponges from NW Spain on a neuroblastoma cell line. Environ Toxicol Pharmacol 2011;32:430-7.

Lakshmi V, Mishra SK, Srivastava S, Chaturvedi A, Srivastava MN, Shukla PK. Antifungal activityof marine sponge Haliclona exigua (Krikpatrick). J Med Mycol 2010;20:31-3.

Li H, Shigeki S, Fusetani N. Simple antifungal metabolites from a marine sponge, Halichondria sp. Comp Biochem Physiol Part B Comp Biochem 1994;107:261-4.

Sofowora A. Medicinal Plants and Traditional Medicinal in Africa. 2nd ed. Sunshine House, Ibadan, Nigeria: Spectrum Books Ltd., Screening Plants for Bioactive Agents; 1993. p. 134-56.

Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd ed. New York: Chapman and Hall; 1973. p. 279.

Smolenski SJ, Silinis H, Farnsworth NR. Alkaloid screening. V. Lloydia 1974;37:506-36.

Jana S, Shekhawat GS. Phytochemical analysis and antibacterial screening of in vivo and in vitro extracts of Indian medicinal herbs: Anethum graveolens. Res J Med Plants 2010;4(4):206-12.

Ayoola GA, Coker AB, Adesegun SA, Adepoju-Bello AA, Obaweya K, Ezennia EC, et al. Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in Southwestern Nigeria. Trop J Pharm Res 2008;7(3):1019-24.

Sureshkumar CA, Varadharajan R, Muthumani P, Meera R, Devi P, Kameswari B. Pharmacognostic and preliminary phytochemical investigations on the stem of Saccharum spontaneum. J Pharm Sci Res 2009;1(3):129-36.

Kolawole OM, Oguntoye SO, Agbede O, Olayemi AB. Studies on the efficacy of Bridelia ferruginea Benth.bark extract in reducing the coliform load and BOD of domestic waste water. Ethnobot Lealf 2006;10:228-38.

Massiot G, Lavaud C, Besson V, Men-Olivier LL, van Binstt G. Saponins from aerial parts of alfalfa (Medicago sativa). J Agric Food Chem 1997;39:78-82.

Available from: http://www.dermaviduals.com/cms/upload/Publikationen _ english/KI-12-14-Alkaloide-engl.pdf.

Arct J, Pytkowska K. Flavonoids as components of biologically active cosmeceuticals. Clin Dermatol 2008;26(4):347-57.

Available from: http://www.dowcorning.co.kr/ko_KR/content/about/aboutehs/EHSPortalFiles/GPS_Safety_Report_540-97-6_D6.pdf.

Karwati A, Nomura J, Ramli N, Wahyudi AT. Cytotoxicity of crude extract from sponge-associated bacteria against molt4 leukemic cell lines through apoptosis. Int J Pharm Pharm Sci 2015;7(12):246-9.

Sugappriya M, Sudarsanam D. Free radical screening activity of marine sponge Aurora globostellata. Asian J Pharm Clin Res 2016;9(4):210-2.

Krishnaveni M. GC-MS/MS analysis of brown rice. Asian J Pharm Clin Res 2015;8(5):73-75.

Sutharsana SL, Sankareswaran SK, Muthuselvam P, Ramanathan G. Characterization and anticancer activity evaluation of fatty acid metabolite from marine cyanobacteria in the Southwest coast of Tamil Nadu, India. J Chem Pharm Res 2016;8(1S):160-9.

Jasim H, Hussein AO, Hameed IH, Kareem MA. Characterization of alkaloid constitution and evaluation of antimicrobial activity of Solanum nigrum using gas chromatography mass spectrometry (GC-MS). J Pharmacogn Phytother 2015;7(4):57-73.