COW URINE DISTILLATE AS A BIOENHANCER FOR ANTIMICROBIAL & ANTIPROLIFERATIVE ACTIVITY AND REDISTILLED COW URINE DISTILLATE AS AN ANTICLASTOGEN AGENT
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i10.18879Keywords:
Cow urine distillate, Redistilled cow urine distillate, Bioenhancer, Antimicrobial activity, Antiproliferative activity, Anticlastogen activityAbstract
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 Objective: The objective of this study was to prove that cow urine distillate (CUD) is a bioenhancer for antimicrobial activity and antiproliferative activity, redistilled CUD (RCUD) as an anticlastogen agent.
Methods: The antimicrobial activity of rifampicin with CUD at different concentrations was determined against pathogenic Escherichia coli by well puncture method. The Penicillin and ciprofloxacin in combination with CUD at different/increasing concentrations against pathogenic E. coli culture were also determined by disc diffusion method. Sulforaphane (ACA) as an anticancer agent was extracted from cruciferous vegetables and purified by high-performance liquid chromatography. The Breast cancer cell lines (MCF-7) were treated with anticancer agents along with CUD in increasing concentrations. The anticlastogenic activity of RCUD in human peripheral lymphocytes was tested with clastogens such as manganese dioxide and hexavalent chromium.
Results: CUD showed to enhance the antimicrobial activity of rifampicin with 20 μl concentration by well puncture method; penicillin with increasing concentration of up to 80 μl and ciprofloxacin up to 80 μl, respectively, by disc diffusion method. The rate of degeneration of breast cancer cell lines (MCF-7) was increased with increasing concentration of CUD. Clastogen (MnO2) of 10 μl with 200 μl of RCUD showed effective anticlastogenic activity in agarose gel electrophoresis as the activity of clastogen decreased with increasing concentration of RCUD.
Conclusion: CUD acts as a bioenhancer to increase antimicrobial and antiproliferative activity. RCUD showed a high level of anticlastogenic activity toward clastogen. Thus, cow urine is found to have special properties that can be used in combination with different therapeutic agents to cure several diseases such as tuberculosis, leprosy, and cancer. Further in vivo and clinical studies are required to confirm its therapeutic efficacy
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References
Shah CP, Patel DM, Dhami PD, Kakadia J, Bhavsar D, Vachhani UD, et al. In vitro screening of antibacterial activity of cow urine against pathogenic human bacterial strains. Int J Curr Pharm Res 2011;3(2):91-2.
Khanuja SP, Kumar S, Shasany AK, Darokar MP, Singh M, Sinha P et al. Use of Bioactive Fraction from Cow Urine Distillate (‘Go-Mutra’) as a Bio-Enhancer of Anti-Infective, Anti-Cancer Agents and Nutrientsâ€. (US Patent No. 6,410,059).
Shah E. Herbal Composition in Cow Urine Distillate. US Patent No. 5693327; 1997.
Mohanty I, Senapati MR, Jena D, Pallai S. Diversified uses of cow urine. Int J Pharm Pharm Sci 2014;6:20-2.
Krishnamurthi K, Dutta D, Devi SS, Chakrabarti T. Protective effect of distillate and redistillate of cow’s urine in human polymorphonuclear leucocytes challenged with established genotoxic chemicals. Biomed Environ Sci 2004;17:57-66.
Chauhan RS, Singh BP, Singhal LK. Immunomodulation with Kamdhenu ark in mice. J Immunol Immunolpathol 2001;71:89-92.
Edwin J, Sheej E, Vaibhav T, Rajesh G, Emmanuel T. Antioxidant and antimicrobial activities of cow urine. Glob J Pharmacol 2008;2(2):20-2.
Chawla PC. Resorine a novel CSIR drug curtails TB treatment. CSIR News 2010;60:52-4.
Vats S, Kumar R, Negi S. Natural food that meet antibiotics resistance challenge: In vitro synergistic antimicrobial activity of Azadirachta indica, Terminalia chebula, Piper nigrum and photoactivated cow urine. Asian J Pharm Biol Res 2012;2:122-6.
Arunkumar S, Muthuselvam M, Rajasekran R. Antimicrobial activities of cow urine distillate against some clinical pathogens. Glob J Pharmacol 2010;4(1):41-4.
Shaw SL, Mitloehner FM, Jackson W, Depeters EJ, Fadel JG, Robinson PH et al. Volatile organic compound emissions from dairy cows and their wastes as measured by proton transfer-reaction mass spectrometry. Environ Sci Technol 2007;14:1310-6.
Dharma K, Rathore R, Chauhan R, Panchagavya S. (Cow pathy): An overview. Int J Cow Sci 2005;1(2):26-9.
Bhalli JA, Shaddock JG, Pearce MG, Dobrovolsky VN. Sensitivity of the pig-an assay for detecting gene mutations in rats exposed acutely to strong clastogens. Mutagenesis 2013;28:447-55.
Dutta D, Devi SS, Krishnamurthi K, Chakrabarti T. Anticlastogenic effect of redistilled cow’s urine distillate in human peripheral lymphocytes challenged with manganese dioxide and hexavalent chromium. Biomed Environ Sci 2006;19:487-94.
Sathasivam A, Muthuselvam M, Rjendran R. Antimicrobial activities of cow urine distillate against some clinical pathogens. Glob J Pharmacol 2010;4:41-4.
Zhang Y, Talalay P, Cho CG, Posner G. A major inducer of anticarcinogenic protective enzymes from Broccoli: Isolation and elucidation of structure. Proc Natl Acad Sci USA 1992;89:2399-403.
Kushad MM, Brown AF, Kurilich AC, Juvik JA, Klein BP, Wallig MA, et al. Variation of glucosinolates in vegetable crops of Brassica oleracea. J Agric Food Chem 1999;47(4):1541-8.
Kintzios SE. Terrestrial plant-derived anticancer agents and plant species used in anticancer research. Crit Rev Plant Sci 2006;25(2):79-113.
Pathak ML, Kumar A. Gomutra-descriptive study. Sachitra Ayurveda 2003;7:81-4.
Lakshmi P. Potential source of fresh and photoactivated gomutra for study of antioxidant and antipathogenic activities against various pathogens. Int J Pharm Pharm Sci 2014;8:459-62.
Wate SP, Dhanjode DP, Duragkar NJ, Tajne MR. Antioxidant potential of cow urine and its fractions: A comparative study. Int J Univ Pharm Life Sci 2011;1(1):146-54.
Deshmukh SS, Rajgure SS, Ingole SP. Antifungal activity of cow urine. IOSR J Pharm 2012;2(5):27-30.
Zhang Y, Li J, Tang L. Cancer-preventive isothiocyanates: Dichotomous modulators of oxidative stress. Free Radic Biol Med 2005;38(1):70-7.
Hao L, Chun-Fang L, Qi-Peng Y, Vriesekoop F. Separation and purification of sulforaphane from Broccoli seeds by solid phase extraction and preparative high-performance liquid chromatography. J Agric Food Chem 2007;55(20):8047-53.
Matusheski NV, Wallig MA, Juvik JA, Klein BP, Kushad MM, Jeffery EH. Preparative HPLC method for the purification of sulforaphane and sulforaphane nitrile from Brassica oleracea. J Agric Food Chem 2001;49:1867-72.
Ipsita M, Manas RS, Deepika J, Santwana P. Diversified uses of cow urine. Int J Pharm Pharm Sci 2014;6(3):20-2.
Vermeulen M, van den Berg R, Freidig AP, van Bladeren PJ, Vaes WH. Association between consumption of cruciferous vegetables and condiments and excretion in urine of isothiocyanate mercapturic acids. J Agric Food Chem 2006;54:5350-8.
Randhawa GK. Cow urine distillate as bioenhancer. J Ayurveda Integr Med 2010;1:240-1.
Vijayalakshmi R, Saranya VT. Effect of go-mutra†on plant growth and its antifungal and antibacterial activity. J Herbal Sci Technol 2010;6:6-11.
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