ANTIMICROBIAL ACTIVITY OF A PROTEASE INHIBITOR ISOLATED FROM THE RHIZOME OF CURCUMA AMADA

Sangeetha R; Padmini R

doi:10.22159/ajpcr.2017.v10i9.18257

Authors

Sangeetha R Department of Biochemistry, School of Life Sciences, Vels University, Chennai - 600 117, Tamil Nadu, India
Padmini R Department of Biochemistry, School of Life Sciences, Vels University, Chennai - 600 117, Tamil Nadu, India

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i9.18257

Keywords:

Curcuma amada rhizome, Protease inhibitor, Antibacterial activity, Minimal inhibitory concentration, Minimal bactericidal concentration

Abstract

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Â Objective: Protease inhibitors (PIs) are effective antimicrobial agents, and this study was aimed to study the antibacterial efficacy of a PI isolated from the rhizome of Curcuma amada.

Methods: A proteinaceous protease inhibitor was isolated from the rhizome of C. amada and purified by Sephadex G-50 gel permeation chromatography. The purified inhibitor was denoted as Curcuma amada protease inhibitor (CAPI). The antibacterial effect of CAPI against Gram-positive and Gram-negative bacteria, the minimal inhibitory concentration (MIC), and minimal bactericidal concentration (MBC) of CAPI was studied in vitro, and the membrane disruption activity of CAPI was also analyzed.

Results: CAPI was effective against both Gram-positive and Gram-negative bacteria, with slightly higher concentrations required for Gram-negative bacteria. The MIC ranged from 75 to 100 Î¼g/ml and the MBC ranged from 100 to 125 Î¼g/ml of CAPI. The study of membrane disruption by CAPI revealed the release of cell contents, namely, reducing sugars and proteins from the bacterial cell.

Conclusion: A PI was effectively isolated from the rhizome of C. amada, and the isolated inhibitor proved to be a promising antibacterial agent.

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References

Kim JY, Park SC, Hwang I, Cheong H, Nah JW, Hahm KS, et al. Protease inhibitors from plants with antimicrobial activity. Int J Mol Sci 2009;10(6):2860-72.

Park Y, Choi BH, Kwak JS, Kang CW, Lim HT, Cheong HS, et al. Kunitz-type serine protease inhibitor from potato (Solanum tuberosum L. cv. Jopung). J Agric Food Chem 2005;53(6):6491-6.

Arulpandi I, Sangeetha R. Antibacterial activity of fistulin: A protease inhibitor purified from the leaves of Cassia fistula. ISRN Pharm 2012;2012:584073.

Bijina B, Chellappan S, Krishna JG, Basheer SM, Elyas KK, Bahkali AH, et al. Protease inhibitor from Moringa oleifera with potential for use as therapeutic drug and as seafood preservative. Saudi J Biol Sci 2011;18(3):273-81.

Policegoudra RS, Chandrashekhar RH, Aradhya SM, Singh L. Cytotoxicity, platelet aggregation inhibitory and antioxidant activity of Curcuma amada Roxb. Extracts. Food Technol Biotechnol 2011;49 Suppl 2:162-8.

Policegoudra RS, Aradhya SM, Singh L. Mango ginger (Curcuma amada Roxb.) A promising spice for phytochemicals and biological activities. J Biosci 2011;36 Suppl 4:739-48.

Rahman RN, Geok LP, Basri M, Salleh AB. Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K. Bioresour Technol 2005;96(4):429-36.

Mohammed NS, Srinivasulu A, Chittibabu B, Rao VU. Isolation and purification of antibacterial principle from Avicennia marina L in methanol. Int J Pharm Pharm Sci 2015;7(1):38-41.

Ribeiro JK, Cunha DD, Fook JM, Sales MP. New properties of the soybean trypsin inhibitor: Inhibition of human neutrophil elastase and its effect on acute pulmonary injury. Eur J Pharmacol 2010;644(1-3):238-44.

Mayasa V, Rasal VK, Unger BS. Evaluation of phenol content, antioxidant and proteinase inhibitory activity of plant derived protease inhibitors of eight anti-diabetic plants. Asian J Pharm Clin Res 2016;9(3):215-9.

Krishnan MV, Murugan K. Solanum protease inhibitors and their therapeutic potentialities. Int J Pharm Pharm Res 2016;8(12):14-21.

Paiva PM, Pontual EV, Coelho LC, Napoleao TH. Protease inhibitors from plants: Biotechnological insights with emphasis on their effects on microbial pathogens. In: Mendez-Vilas A, editor. Microbial Pathogens and Strategies for Combating Them: Science. Badajoz: Technology and Education, Formatex; 2013.

Kim JY, Park SC, Kim MH, Lim HT, Park Y, Hahm KS. Antimicrobial activity studies on a trypsin-chymotrypsin protease inhibitor obtained from potato. Biochem Biophys Res Commun 2005;330(3):921-7.

Sukandar EY, Fidrianny I, Kamil A. In situ antibacterial activity of Kaempferia pandurata (Roxb.) rhizomes against Staphylococcus aureus. Int J Pharm Pharm Sci 2015;7(2):239-44.