DETERMINATION OF PHYTOCOMPONENTS IN ETHANOL EXTRACT OF BRASSICA OLERACEA - USING GAS CHROMATOGRAPHY–MASS SPECTROSCOPY TECHNIQUE
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i10.26857Keywords:
Brassica oleracea, Nil, Tetratetracontane, Stigmasterol, Phytocompounds, antioxidantAbstract
Objectives: Bioactive components determined by plants are known to have a broad application in the medical field. The focus of this study is to recognize the phytochemicals in the ethanol extract of Brassica oleracea by gas chromatography–mass spectroscopy (GC–MS).
Methods: B. oleracea was collected, dried, and powdered well. The extraction was done with the solvent ethanol. The extract was exposed to column of GC-MS-QP 2010 (SHIMADZU) column Db 30.0 (0.25 μm in diameter, 0.25 μm thick).
Results: GC–MS result provides the chromatogram with different peaks obtained at a different retention time shows the presence of various biocompounds. Some of the identified bioactive compounds are n-hexadecanoic acid (12.99%), phytol (2.40%), Vitamin E (3.38%), tetratetracontane (2.15%), stigmasterol (2.03%), and isophytol.
Conclusion: The GC–MS study of the ethanol extract of B. oleracea reveals the existence of many potential compounds that can be utilized in the pharmaceutical industry, including the use of anti-inflammatory, antiarthritic, anticoronary, and antidiabetic agents.
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