EDEMA-REDUCING ACTIVITY OF SARGASSUM CRASSIFOLIUM Î’-GLUCAN (LAMINARAN) ON EDEMA-INDUCED RATTUS NORVEGICUS

Anies Chamidah; Asep A Prihanto; Hardoko Hardoko

doi:10.22159/ajpcr.2017.v10i7.18523

Authors

Anies Chamidah Department of Fishery Product Technology, Faculty of Fisheries and Marine Science, Brawijaya University, Indonesia.
Asep A Prihanto Department of Fishery Product Technology, Faculty of Fisheries and Marine Science, Brawijaya University, Indonesia. http://orcid.org/0000-0003-4180-1325
Hardoko Hardoko Department of Fishery Product Technology, Faculty of Fisheries and Marine Science, Brawijaya University, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i7.18523

Keywords:

Nil, Edema, Sargassum crassifolium, Carrageenan, Edema

Abstract

Objectives: The aim of this study is to investigate anti-inflammatory activity of Î²-glucan (laminaran) of brown algae, Sargassum crassifolium which was extracted by using two different methods, Î²-glucan laminaran acid extract (LAE) and laminaran modified extract (LME).

Methods: Î²-glucan (laminaran) of brown algae, S. crassifolium have been extracted using two different methods, namely, Î²-glucan LAE and LME. The edema reducing activity of the extracts was investigated with carrageenan- induced leg edema male of Rattus norvegicus as a model.

Results: The swelling of edema was strongly inhibited by the treatment of LME with a percentage of 81.62% after 3rd hr. It also found that the size of edema were inhibited by all treatments with the value ranging from 25.49% to 81.68%. Smallest edema size was found on LME treatment, with a size of 0.68Â±0.09 mm where the control was about 0.92 mm. Furthermore, all Î²-glucan-treated mice showed lower edema size compared to control.

Conclusion: It was obvious that all type of laminaran had anti-inflammatory activity. LME is the best method for production of Î²-glucan (laminaran) with high anti-inflammatory activity. Hence, in this study, we reported a new anti-inflammatory agent derived from S. crassifolium.

Downloads

Download data is not yet available.

References

Malve H. Exploring the ocean for new drug developments: Marine pharmacology. J Pharm Bioallied Sci 2016;8(2):83-91.

Prihanto AA, Wakayama M. Marine microorganism: An underexplored source of L-asparaginase. Adv Food Nutr Res 2016;79:1-25.

Chizhov AO, Dell A, Morris HR. Structural analysis of laminarans by MALDI and FAB mass spectrometry. Carbohydr Res 1998;310(3):203â€‘10.

Rioux LE, Turgeon SL, Beaulieu M. Effect of season on the composition of bioactive polysaccharides from the brown seaweed Saccharina longicruris. Phytochemistry 2009;70(8):1069-75.

Kurniatanty I, Tan MI, Ruml T, Sumarsono SH. Potential cell proliferation inhibitor isolated from Indonesian brown algae (Phaeophyta). Int J Pharm Pharm Sci 2015;7(11):140-3.

Oliveira NM, Meira CL, Aguiar RM, De Oliveira DM, Moura CW, Filho SA. Antitumor effects and characterization of biosynthesized iron oxide nanoparticles using seaweeds of Gulf of Mannar. Int J Pharm Pharm Sci 2015;7:350-3.

Mahat MA, Patil BM. Evaluation of anti-inflammatory activity of methanol extract of Phyllanthus amarus in experimental animal models. Indian J Pharm Sci 2007;69(1):33-6.

Sosa S, Balick MJ, Arvigo R. Screening of the topical anti-inflammatory activity of some Central American plants. J Ethnopharmacol 2002;81(2):211-5.

Amdekar S, Roy P, Singh V, Kumar A, Singh R, Sharma P. Anti-inflammatory activity of lactobacillus on carrageenan-induced paw edema in male wistar rats. Int J Inflam 2012;2012:752015.

Yvin JC, Le Vasseur F, Hudâ€™Homme F. Use of laminarin and oligosaccharides derived therefrom in cosmetics and for preparing a skin treatment drug. US Patent. 59 80 916; 1999.

Mohsen AM, Mohamed SF, Ali FM, El-Sayed OH. Chemical structure and antiviral activity of water-soluble sulfated polysaccharides from Surgassum latifolium. J Appl Sci Res 2007;3(10):1178-85.

Duffy JC, Dearden JC, Rostron C. Design, Synthesis and biological testing of a novel series of anti - inflammatory drugs. J Pharm Pharmacol 2001;531505-14.

Frenkel JK. Adoptive immunity to intracellular infection. J Immunol 1967;98:1309-19.

Nualkaew S, Rattanamanee K, Thongpraditchote S, Wongkrajang Y, Nahrstedt A. Anti-inflammatory, analgesic and wound healing activities of the leaves of Memecylon edule Roxb. J Ethnopharmacol 2009;121(2):278-81.

Winter CA, Risley EA, Nuss GW. Carrageenan induced edema in the hind paw of rat as an assay for anti-inflammatory activity. Proc Soc Exp Biol Med 1962;111:544-7.

Adeyemi OO, Okpo SO, Ogunti OO. Analgesic and anti-inflammatory effects of the aqueous extract of leaves of Persea americana Mill (Lauraceae). Fitoterapia 2002;73(5):375-80.

Ratheesh M, Helen A. Anti-inflammatory activity of Ruta graveolens Linn on carrageenan induced paw edema in Wistar male rats. Afr J Biotechnol 2007;6(10):1209-11.

Di Rosa M, Giroud JP, Willoughby DA. Studies on the mediators of the acute inflammatory response induced in rats in different sites by carrageenan and turpentine. J Pathol 1971;104(1):15-29.

Panthong A, Pangaoop R, Khonsung P, Chiranthanut N, Nuntasaen N, Jaitheerapapkul S, et al. Anti-inflammatory and analgesic effects of the methanol extract from garcinia wallichii choisy in animal models. Chiang Mai Med J 2009;48(3):105-15.

Carlson RP, Oâ€™Neill-Davis L, Chang J, Lewis AJ. Modulation of mouse ear edema by cyclooxygenase and lipooxygenase inhibitors and other pharmacologic agents. Agents Actions 1985;17:197-204.