BIOLOGICAL ACTIVITIES OF GARCINIA MANGOSTANA
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i9.18585Keywords:
Antibacterial activity, Antifungal activity, Phytochemical assay, Antioxidant activity, Nil, Garcinia mangostanaAbstract
Â
 Objective: The aim of the present study was to investigate in vitro antibacterial, antifungal, and antioxidant activities of different solvent extracts of Garcinia mangostana leaves.
Methods: The powdered leaf was subjected to sequential extraction using hexane, ethyl acetate (EA), and methanol. The extracts were subjected to quantitative and qualitative phytochemical analysis, antimicrobial, and antioxidant activities. The best solvent extract was subjected to gas chromatography–mass spectrometry (GC-MS) analysis.
Results: The highest activity was recorded in EA extract which was subjected to GC-MS analysis revealing the presence of squalene (17.09%).
Conclusion: From this present study, we conclude that EA is the best solvent for extracting antimicrobial and antioxidant compounds from the leaves of G. mangostana.
Downloads
References
Attele AS, Zhou YP, Xie JT, Wu JA, Zhang L, Dey L, et al. Antidiabetic effects of Panax ginseng berry extract and the identification of an effective component. Diabetes 2002;51(6):1851-8.
Gryglewski RJ, Korbut R, Robak J, Swies J. On the mechanism of antithrombotic action of flavonoids. Biochem Pharmacol 1987;36(3):317-22.
Akao Y, Nakagawa Y, Iinuma M, Nozawa Y. Anti-cancer effects of xanthones from pericarps of mangosteen. Int J Mol Sci 2008;9(3):355-70.
Kanchanapoom K, Kanchanapoom M. Mangosteen. In: Shaw PE, Chan HT Jr, Nagy S, editors. Tropical and Subtropical Fruits. Vol. 15. Auburndale, Florida: AG Science, Inc.; 1998. p. 68-74.
Saito M, Ogino S, Kubo K, Nagata J, Takeuchi M. High dose of Garcinia cambogia is effective in suppressing fat accumulation in developing male Zucker obese rats, but highly toxic to the testis. Food Chem Toxicol 2005;43(3):411-9.
Gutierrez-Orozco F, Failla ML. Biological activities and bioavailability of mangosteen xanthones: A critical review of the current evidence. Nutrients 2013;5(8):3163-83.
Akao Y, Nakagawa Y, Iinuma M, Nozawa Y. Anti-cancer effects of xanthones from pericarps of mangosteen. Int J Mol Sci 2008;9(3):355-70.
Bindu AR, Rosemary J, Akhila S. Antimicrobial activity screening of M. minuta extracts. Int J Pharm Pharm Sci 2014;6:581-3.
Sunil C, Ignacimuthu S, Agastian P. Antidiabetic effect of Symplocos cochinchinensis (Lour.) S. Moore. In Type 2 diabetic rats. J Ethnopharmacol 2011;134:298-304.
Glorybal L, Kannana KB, Arasu MV, AL-Dhabi NA, Agastian P. Some biological activities of Epaltes divarcata L.-an in vitro study annals os clinical microbiology and antimicrobials. Ann Clin Microbiol Antimicrob 2015;14:1-14.
Nimal Christhudas IV, Praveen Kumar P, Sunil C, Vajravijayan S, Lakshmi Sundaram R, Jenifer Siril S, et al. In vitro studies on a-glucosidase inhibition, antioxidant and free radical scavenging activities of Hedyotis biflora L. Food Chem 2013;138(2-3):1689-95.
Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolke RH. Manual of Clinical Microbiology. 6th ed. Washington, D.C: American Society for Microbiology; 1995.
Pinto E, Pina-Vaz C, Salgueiro L, José Gonçalves M, Costa-de-Oliveira S, Cavaleiro C, et al. Antifungal activity of the essential oil of Thymus pulegioides on Candida, Aspergillus and dermatophyte species. J Med Microbiol 2006;55:1367-73.
Pfaller MA, Messer SA, Coffmann S. Comparison of visual and spectrophotometric methods of MIC endpoint determinations by using broth microdilution methods to test five antifungal agents, including the new triazole D0870. J Clin Microbiol 1995;33(5):1094-7.
Wadood A, Ghufran M, Jamal SB, Naeem M, Khan A, Ghaffar R, et al. Phytochemical analysis of medicinal plants occurring in local area of Mardan. Biochem Anal Biochem 2013;2:1-4.
Hepsibah AH, Jothi GJ. A comparative study on the effect of solvents on the phytochemical profile and biological potential of Ormocarpum cochinchinense Auct. Non (Lour.) Merrill. Int J Pharm Pharm Sci 2017;9:67-72.
Slinkard K, Singleton VL. Total phenol analyses: Automation and comparison with manual methods. Am J Enol Viticult 1977;8:4955.
Hanato T, Kagawa H, Yasuhara T, Okuda T. Two new flavonoids and other constituents in licorice root: Their relative astringency and radical scavenging effects. Chem Pharm Bull (Tokyo) 1988;36:2090-7.
Elizabeth K, Rao MN. Oxygen scavenging activity of curcumin. Int J Pharm 1990;58:237-40.
Smirnoff N, Cumbes QJ. Hydroxyl radial scavenging activity of compatible solutes. Phytochemistry 1989;25:1057-60.
Krishnaiah D, Sarbatly R, Bono A. Phytochemical antioxidants for health and medicine: A move towards nature. Biotechnol Mol Biol Rev 2007;1:97-104.
Marxen K, Vanselow KH, Lippemeier S, Hintze R, Ruser A, Hansen UP. Determination of DPPH radical oxidation on caused by metabolic extracts of some microalgal species by linear regression analysis of spectrophotometric measurements. Sensors 2007;7:2080-95.
Aruoma OI. Free radicals, oxidative stress and antioxidants in human health and disease. J Am Oil Chem Soc 1998;75:199-212.
Steer P, Millgård J, Sarabi DM, Basu S, Vessby B, Kahan T, et al. Cardiac and vascular structure and function are related to lipid peroxidation and metabolism. Lipids 2002;37(3):231-6.
Arora S, Brits E, Kaur S, Kaur K, Sohi RS, Kumar S, et al. Evaluation of genotoxicity of medicinal plant extracts by the comet and VITOTOX tests. J Environ Pathol Toxicol Oncol 2005;24(3):193-200.
Inglis TJ. Microbiology and infection. A Clinically Oriented Core Text with Self-Assessment. New York: Churchill Livingstone, Long Man Singapore Publishers (Pte)Ltd; 1996.
Bannister BA, Begg NT, Gillespie SH. Infectious Disease. 2nd ed. Osney Mead, Oxford: Blackwell Science Ltd.; 2000.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
