EVALUATION OF ANTIMICROBIAL EFFICIENCY AND ΑLPHA-GLUCOSIDASE INHIBITION OF RUBUS ELLIPTICUS SMITH. LEAF EXTRACTS AND ITS PHYTOCHEMICAL ANALYSIS
Abstract
Â
Objective: The aim of this study was to investigate phytochemicals, antimicrobial and α-glucosidase inhibition effects of Rubus ellipticus.
Methods: Various solvent extracts were investigated for its phytochemical analysis. Total phenol content (TPC) and total flavonoid content (TFC) were
determined by using standard methods. The extracts of R. ellipticus were tested for antimicrobial activity and in vitro α-glucosidase inhibition effect.
Results: The phytochemical tests revealed the presence of phytocontituents significantly in methanol extract with high TPC and TFC. Methanol extract
showed significant antibacterial activity against Gram-positive and negative strains, as well as a strong antifungal activity. The maximum inhibition
zone is found against Staphylococcus aureus, Micrococcus luteus and methicillin-resistant S. aureus (MRSA) (22, 17 and 18 mm) respectively. Against
fungus the inhibition zone ranged between 19 mm and 23 mm. Methanolic extract showed minimum inhibitory concentration value with 32.5 μg/ml
against S. aureus, 62.55 μg/ml against MRSA, Aspergillus flavus, Trichophyton mentagrophytes, Trichophyton rubrum 15 μg/ml against Aspergillus niger.
The methanol extracts and standard acarobose showed significant α-glucosidase inhibition effect with 86.14% and 92.46%.
Conclusions: Correlation achieved by antimicrobial activity and α-glucosidase inhibition of R. ellipticus and its chemical composition insinuates that
the activities may be easily ascribed to phenolic compound and flavonoids present in the high percentage in the leaf. Thus, our findings could provide
a basis of future studies on R. ellipticus leaves used in food and pharmaceutical applications.
Keywords: Rubus ellipticus, Phytochemical, Phenolic and flavonoid content, antimicrobial activity, α-glucosidase inhibition.
Downloads
References
Abdullahi SK, Lawal GH. Phytochemical and microbial screening of Parkinsonia aculeata L. leaves. Int J Drug Dev & Res 2010; 2(1):1-American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care2010; 34, S62–S69.
Ang JY, Ezike E, Asmar BI. Antibacterial resistance. Indian Journal of Pediatrics 2004; 71(3):229-239.
Balandrin MF, Klocke JA, Wurtele ES, Bollinger WH. Natural plant chemicals: Sources of Industrial and Medicinal materialsâ€. Sci 1985; 228: 1154- 1160.
Battu GR, Ethadi S, Prayaga Murthy P, VS Praneeth D, Mallikarjuna Rao T. In-vitro antibacterial activity and preliminary phytochemical screening of three algae from Visakhapatnam Coast, Andhra Pradesh, India. Int J Pharm Pharm Sci 2011; 3(4):399-401.
Bauer AW, Kirby WM, Sheries JC, Turck M. Antibiotic susceptibility testing by a Standardized single disc method. Am. Jclin Pathol 1966; 45:149-158.
Dahlqvist A. Method for assay of intestinal disaccharidases. Analytical Biochemistry 1964. 7, 18–25.
Dimayuga RE & Garcia SK. Antimicrobial screening of medicinal plants from Baja California Sur. Mexico. Journal of Ethnopharmacology 1991; 31: 181-192.
Duraipandiyan V, Ayyanar M, Ignacimuthu S. Antimicrobial activity of some ethnomedicinal plants used by Paliyar tribe from Tamil Nadu, India. Complementary and Alernative Medicine 2006; 6: 35.
Duraipandyan V, Ignachimuthu S. Antibacterial and antifungal activity of Cassia fistula L. An ethnomedicinal plant. Journal of Ethnopharmacology 2007; 112; 590–594.
Erdemoglu N, Kupeli, Yesilada E. J. Ethnopharmacol.2003; 89:123-129.
Finn CE. Rubus spp, blackberry. In: Janick J, Paull, RE. (Eds.). The Encyclopedia of Fruits and Nuts. CABI: Cambridge: MA. 2008; 348–351.
George BP, Parimelazhagan T, Saravanan, S. Anti-inflammatory, analgesic and antipyretic activities of Rubus ellipticus smith. leaf methanol extract 2013; 5(2).
Hassan MM, Oyewale AO, Amupitan JO, Abduallahi MS, Okonkwo EM. Preliminary phytochemical and antibacterial investigation of crude extracts of the root bark of Detarium microcarpum. J Chem Soc Nigeria 2004; 29: 26-29.
Matsui T, Ogunwande IA, Abesundara KJM, and Mat-sumoto K, Anti-hyperglycemic potential of natural products,†Mini-Reviews in Medicinal Chemistry 2006; 6( 3): 349–356.
Panda SK, Padhi LP, Mohanty G. Antibacterial activities and phytochemical analysis of Cassia fistula (Linn.) leaf.J Adv Pharm Technol Res 2011; 2(1): 62-67.
Ramesh Babu K, Maddirala Dilip R, Vinay Kumar K, Shaik Sameena F, Tiruvenkata Kumar EG, Swapna S, Ramesh B, Rao CA. Antihyperglycemic and antihyperlipidemic activities of methanol:water (4:1) fraction isolated from aqueous extract of Syzygium alternifolium seeds in streptozotocin induced diabetic rats. Food Chem. Toxicol. 2010; 48: 1078–1084.
Sarala Saklani, Subash Chandra, Badoni PP, Sandhya Dogra .Antimicrobial activity, nutritional profile of wild edible fruit of Rubus ellipticus. Int.J.Med. Aro. Plants. 2012; 2(2): 269-274.
Saravana kumar P, Duraipandyan V, Ignacimuthu S. Isolation, screening and partial purification of antimicrobial antibiotics from soil Streptomyces sp.SCA7. Kaohsiung Journal of Medical Sciences 2014; 30 (9) 435-446.
Siddhuraju P and Becker K. Studies on antioxidant activities of mucuna seed (Mucuna pruriens var. utilis) extracts and certain non-protein amino/imino acids through invitro models. J. Sci. Food. Agric 2003; 83: 1517-1524.
Stanojevic LP, Stankovic MZ, Nikolic VD, Nikolic LB. Anti-oxidative and antimicrobial activities of Hieracium pilosella L. extracts. J. Serb. Chem. Soc 2008; 73: 531–540.
Tekeli Y, Zengin G, Aktumsek A, Sezgin M, Torlak E. Antibacterial activities of extracts from twelve Centaurea species from Turkey. Archives of Biological Sciences 2011; 63(3): 685-690.
Uprety Y, Ram C, Asselin PH, Boon E. Plant biodiversity and ethnobotany inside the projected impact area of the Upper Seti Hydropower Project, Western Nepal. Environ Dev Sustain. 2011; 13:463–492.
Usman H, Osuji JC. Phytochemical and in vitro anti microbial assay of the leaf extract of Newbouldia leavis. Afr J Trad CAM 2007; 4: 476-480.
WHO.Traditional Medicine Strategy 2002-2005. WHO Publications 2002;1-6.
Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes: estimates for the year 2000 an projections for 2030. DiabetesCare 27 2004; 1047–1053.
Zhishen J., Mengecheng T and Jianming W. The determination of flavonoid contents on mulberry and their scavenging effects on superoxide radical. Food. Chem. 1999; 64: 555-559.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.