THE UNEXPLORED ROLES OF PROBIOTIC BACTERIA: IN VITRO ANTI-INFLAMMATORY AND ANTHELMINTIC ACTIVITY OF ENTEROCOCCUS FAECIUM BM10 KY788342 AND LACTOBACILLUS CASEI GM10 KY794586
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i9.19413Keywords:
Lactic acid bacteria, Anti-inflammatory, Anthelmintic, Intracellular cell-free extractAbstract
Â
 Objective: The aim of the present study was to evaluate the in vitro anti-inflammation activity and anthelmintic potential of two novel isolated probiotic strains through Enterococcus faecium BM10 KY788342 and Lactobacillus casei GM10 KY794586.
Methods: In vitro anti-inflammatory activity was evaluated using protein denaturation inhibition method. Pheretima posthuma was used as a suitable in vitro model, and time of paralysis and death were used as parameters to evaluate anthelmintic potential of probiotic strains.
Results: Lyophilized solutions of L. casei GM10 showed significant protein denaturation inhibition (56.20±0.86%) followed by E. faecium BM10 (52.28±0.31%) comparable to diclofenac (93.62±1.39%) at the maximum concentration of 250 μg/ml. Intracellular cell-free extract of E. faecium BM10 showed a strong anthelmintic (vermicidal) activity (6±0.23 minutes), followed by L. casei GM10 (9±0.05 minutes) comparable to piperazine citrate (20±0.422 minutes) and albendazole (24±0.43 minutes) at the maximum concentration of 100 mg/ml.
Conclusion: Results of the present study concluded that both tested lactic acid bacteria strains exhibited significant in vitro anti-inflammatory activity and can be used as potent and safe anthelmintic agent.
Downloads
References
Ménard S, Candalh C, Bambou JC, Terpend K, Cerf-Bensussan N, Heyman M. Lactic acid bacteria secrete metabolites retaining anti-inflammatory properties after intestinal transport. Gut 2004;53(6):821-8.
Amdekar S, Roy P, Singh V, Kumar A, Singh R, Sharma P. Anti-inflammatory activity of Lactobacillus on carrageenan-induced paw edema in male wistar rats. Int J Inflam 2012;2012:752015.
Divyashri G, Krishna G, Muralidhara, Prapulla SG. Probiotic attributes, antioxidant, anti-inflammatory and neuromodulatory effects of Enterococcus faecium CFR 3003: In vitro and in vivo evidence. J Med Microbiol 2015;64(12):1527-40.
World Health Organization. Soil-Transmitted Helminthiases: Eliminating Soil-Transmitted Helmnthiases as a Public Health Problem in Children. Progress Report; 2012. p. 1-90.
Holden-Dye L, Robert WJ. Anthelmintic drugs and nematicides: Studies in Caenorhabditis elegans. In: Worm Book: The Online Review of C. Elegans Biology. New York, USA: W. H Freeman; 2013. p. 1-22.
Kumar ML, Thippeswamy B, Kuppust IL, Naveenkumar KJ, Shivakumar CK. Evaluation of Bacillus cereus and Bacillus pumilus metabolites for anthelmintic activity. Pharmacognosy Res 2015;7(1):81-4.
Haber CL, Heckaman CL, Li GP, Thompson DP, Whaley HA, Wiley VH. Development of a mechanism of action-based screen for anthelmintic microbial metabolites with avermectinlike activity and isolation of milbemycin-producing Streptomyces strains. Antimicrob Agents Chemother 1991;35(9):1811-7.
Amanto JS, Brooklyn NY, Cvetovich R, Scotch, P. United States Patent No. 19; 1995.
Kar B, Nepal A, Kumar RB, Dolai N, Bhattacharya S, Upal K, et al. Antioxidant and anti-inflammatory properties hymenodictyon excelsum bark. Orient Pharm Exp Med 2013;13(2):103-11.
Chen Q, Kong B, Sun Q, Dong F, Liu Q. Antioxidant potential of a unique LAB culture isolated from Harbin dry sausage: In vitro and in a sausage model. Meat Sci 2015;110:180-8.
Bharti V, Mehta A, Jain N, Singh S, Ahirwal L. In vivo acute toxicity, antibacterial, antiaquatic fungal, antihelminthic activity of Lactobacillus plantarum kp894100 and Lactobacillus acidophilus. Asian J Pharm Clin Res 2016;9(1):1-5.
Opie EL. On the relation of necrosis and inflammation to denaturation of proteins. J Exp Med 1962;115(3):597-608.
Mizushima Y, Kobayashi M. Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins. J Pharm Pharmacol 1968;20(3):169-73.
Williams LA, Connar AO, Latore L, Dennis O, Ringer S, Whittaker JA, et al. The in vitro anti-denaturation effects induced by natural products and non-steroidal compounds in heat treated (immunogenic) bovine serum albumin is proposed as a screening assay for the detection of anti-inflammatory compounds, without the use of anim. West Indian Med J 2008;57(4):327-31.
Carroll IM, Andrus JM, Bruno-Bárcena JM, Klaenhammer TR, Hassan HM, Threadgill DS. Anti-inflammatory properties of Lactobacillus gasseri expressing manganese superoxide dismutase using the interleukin 10-deficient mouse model of colitis. Am J Physiol Gastrointest Liver Physiol 2007;293(4):G729-38.
Saraswathi R, Upadhyay L, Venkatakrishnan R, Meera R, Devi P. Phytochemical investigation, analgesic and anti-inflammatory activity of Abutilon indicum Linn. Int J Pharm Pharm Sci 2011;3:154-6.
Nagore DH, Ghose VK, Patil MJ, Wahile AM. In vitro antioxidant and in vivo anti-inflammatory activity of Cassia sophera Linn. Int J Pharm Pharm Sci 2010;2:113-21.
Giblin-Davis RM, Williams DS, Bekal S, Dickson DW, Brito JA, Becker JO, et al. ‘Candidatus pasteuria usgae’ sp. Nov. An obligate endoparasite of the phytoparasitic nematode Belonolaimus longicaudatus. Int J Syst Evol Microbiol 2003;53:197-200.
Dash GK, Suresh P, Sahu SK, Kar DM, Ganapaty S, Panda SB. Evaluation of Evolvulus alsinoides Linn. For anthelmintic and antimicrobial activities. J Nat Rem 2002;2(2):182-5.
Partap S, Kumar S, Kumar A, Sharma NK, Jha KK. In-vitro anthelmintic activity of Luffa cylindrica leaves in Indian adult earthworm. J Pharmacogn Phytochem 2012;1(2):27-30.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
