MODULATORY ACTIVITY OF BEE POLLEN AGAINST THE TOXICITY OF ANTITUBERCULOSIS DRUGS RIFAMPICIN AND ISONIAZID IN TESTIS OF SPRAGUE DAWLEY RATS

Umesh Bharti; Neelima R Kumar; Jaspreet Kaur

doi:10.22159/ajpcr.2017.v10i9.19701

Authors

Umesh Bharti Department of Zoology, Government College for Girls, Chandigarh, India.
Neelima R Kumar Department of Zoology, Panjab University, Chandigarh, India.
Jaspreet Kaur Department of Biotechnology, UIET, Panjab University, Chandigarh, India

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i9.19701

Keywords:

Bee pollen, testis, histology, oxidative stress, rifampicin, isoniazid

Abstract

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Â Objective: The aim of this study is assessment of protective role of bee pollen in antituberculosis drug (rifampicin and isoniazid)-induced toxicity in testis of Sprague Dawley rats.

Methods: Healthy rats weighing 180Â±20 g were selected for the study. Rats were divided into five groups, i.e., Group A (control), Group B (100 mg/kg body weight/day rifampicin-treated), Group C (rifampicin 100 mg/kg body weight and bee pollen 100 mg/kg body weight), Group D (isoniazid 50 mg/kg body/day treated), and Group E (isoniazid 50 mg/kg body weight/day with bee pollen 100 mg/kg body weight/day) serve as experimental groups.

Results: Aqueous extract of bee pollen when administered along with the antituberculosis drugs (rifampicin, isoniazid) showed significant reduction in the level of malondialdehyde while the activity of superoxide dismutase, glutathione (GSH) reductase, glutathione peroxidase, glutathioneS-transferase, catalase, and GSH was elevated representing the antioxidant potential of bee pollen against the drug-treated groups. Supplementation of bee pollen significantly reduced histological changes in the testis of drug-induced groups such as smaller epithelial height, germ cell loss, and irregular seminiferous tubules to near normal.

Conclusion: Bee pollen has shown the modulatory effect against damage and oxidative stress induced by antituberculosis drugs (rifampicin and isoniazid) in rat testis.

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Author Biographies

Umesh Bharti, Department of Zoology, Government College for Girls, Chandigarh, India.

zoology department,post graduate govt. college for girls,sector-11, Chandigarh ,India

Neelima R Kumar, Department of Zoology, Panjab University, Chandigarh, India.

ZOOLOGY DEPARTMENT, PANJAB UNIVERSITY

Jaspreet Kaur, Department of Biotechnology, UIET, Panjab University, Chandigarh, India

BIOCHEMISTRY DEPARTMENT, PANJAB UNIVERSITY, CHANDIGARH

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