EVALUATION OF BIOACTIVITIES OF MORINDA TINCTORIA LEAVES EXTRACT FOR PHARMACOLOGICAL APPLICATIONS.

Thangavel Sivakumar; Bhagavathi Sundaram Sivamaruthi; Kamaraj Lakshmi Priya; Periyanaina Kesika; Chaiyavat Chaiyasut

doi:10.22159/ajpcr.2018.v11i2.21583

Authors

Thangavel Sivakumar Department of Microbiology, Ayya Nadar Janaki Ammal College (Autonomous), Sivakasi, Tamil Nadu, India.
Bhagavathi Sundaram Sivamaruthi nnovation Center for Holistic Health, Nutraceuticals, and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai-50200, Thailand.
Kamaraj Lakshmi Priya Department of Microbiology, Ayya Nadar Janaki Ammal College (Autonomous), Sivakasi, Tamil Nadu, India.
Periyanaina Kesika Innovation Center for Holistic Health, Nutraceuticals, and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai-50200, Thailand
Chaiyavat Chaiyasut Innovation Center for Holistic Health, Nutraceuticals, and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai-50200, Thailand

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i2.21583

Keywords:

Antimicrobial, Antioxidant, Anti-inflammation, Aqueous extraction, Morinda tinctoria

Abstract

Â Objective: The present study was aimed to prepare Morinda tinctoria leaves extracts with the different solvent system and to evaluate the bioactivities.

Methods: The extracts of M. tinctoria were qualitatively analyzed for the primary phytochemical content. The functional groups of extract were determined by Fourier-transform infrared spectroscopy (FT-IR) analysis. The antimicrobial properties were determined by plate assays. The antioxidant and in vitro anti-inflammatory properties and membrane stabilizing nature of aqueous extract of M. tinctoria (AEM) were measured using a spectrophotometer.

Results: The aqueous, ethanolic, and acetone extracts of M. tinctoria were prepared. AEM contains quinones, steroids, terpenoids, phenols, glycosides, and tannins. FTIR result showed that AEM comprises of alkyl halides, 1Â°, 2Â° amines, aromatics, aliphatic amines, alcohols, carboxylic acids, esters, ethers, and alkanes, saturated aliphatic, and phenolic groups. The antimicrobial property of M. tinctoria varied based on the solvent used for the extraction. About 86.90Â±0.36, 78.58Â±0.13, and 80.33Â±0.09% of total antioxidant capacity, reducing power, and hydrogen peroxide scavenging activity were observed in AEM, respectively. The 1, 1- diphenyl 2-picrylhyorazyl and 2, 2-Azinobis-(3 ethylbenzothiazoline-6-sulfonic acids) assay results indicated 85.20Â±0.50 and 52.41Â±0.60% of free radical scavenging activity in AEM. The protease activity (44.10Â±0.26%) and protein degradation (44.38Â±0.58%) were proscribed by AEM. AEM prevents 69.36Â±0.20% of cell lysis.

Conclusion: The results revealed that the AEM leaves were harmless and enriched with potent bioactive principles, which is further used for food and pharmacological applications.

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