THE TOTAL POLYPHENOLIC CONTENT AND ANTIOXIDANT PROPERTIES OF VARIOUS HONEY AND SUGARS

Chaiyavat Chaiyasut; Periyanaina Kesika; Sartjin Peerajan; Bhagavathi Sundaram Sivamaruthi

doi:10.22159/ajpcr.2018.v11i5.24696

Authors

Chaiyavat Chaiyasut Innovation Center for Holistic Health, Nutraceuticals and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai-50200, Thailand.
Periyanaina Kesika Innovation Center for Holistic Health, Nutraceuticals and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai-50200, Thailand.
Sartjin Peerajan Health Innovation Institute, Chiang Mai-50200, Thailand.
Bhagavathi Sundaram Sivamaruthi Innovation Center for Holistic Health, Nutraceuticals and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai-50200, Thailand. http://orcid.org/0000-0002-5499-8350

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i5.24696

Keywords:

Antioxidant, 2, Nil, Ferric reducing antioxidant potential, Phenolic compound, Sugars, Thai honey

Abstract

Objective: Honey and sugars are rich in polyphenols and known for several health benefits. The quality of the honey varied on the floral source and geographical region. The sugar quality depends on the processing and source. The study aimed to evaluate the total polyphenolic content and antioxidant properties of selected Thai honey and sugar samples.

Methods: Total polyphenol content and antioxidant properties were determined by the colorimetric method, and 2, 2â€²-azino-bis-3-ethylbenzthiazoline- 6-sulfonic acid, ferric reducing antioxidant potential (FRAP), and ferrous ion-chelating assays, respectively.

Results: The honey from Eupatorium odoratum Linn (HEO) (0.75 mg gallic acid equivalent/ml of sample), and meal cane sugar (MCS) (4.13 mg gallic acid equivalent/g of sample) exhibited higher polyphenol content among the honey, and sugar samples, respectively. The refined sugar (RS) showed least phenolic content (0.02 mg gallic acid equivalent/g of sample) when compared to all tested sugars and honey samples. HEO exhibited high Trolox equivalent antioxidant capacity (TEAC; 235.93 mg TEAC per ml sample) among the honey samples. Among the sugars, MCS showed high TEAC (3891.22 mg TEAC per g sample). The honey from Nephelium lappaceum Linn and HEO has superior FRAP value and chelating power, respectively. The RS was least in total antioxidant capacity, FRAP, and chelating power.

Conclusion: The HEO, and MCS and palm sugars are harbored with phenolic content and antioxidants. The refining process drastically diminished the quality of the sugar. The information may help to choose best carbon source for the preparation of fermented beverages with improved functional properties.

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References

Al-Qassemi R, Robinson R. Some special nutritional properties of honey-a brief review. Nutr Food Sci 2003;33:254-60.

Pattamayutanon P, Angeli S, Thakeow P, Abraham J, Disayathanoowat T, Chantawannakul P. Volatile organic compounds of Thai honeys produced from several floral sources by different honey bee species. PLoS One 2017;12:e0172099.

Ball DW. The chemical composition of honey. J Chem Educ 2007;84:1643-6.

Ferreira IC, Aires E, Barreira JC, Estevinho LM. Antioxidant activity of Portuguese honey samples: Different contributions of the entire honey and phenolic extract. Food Chem 2009;114:1438-43.

Erejuwa OO, Gurtu S, Sulaiman SA, Ab Wahab MS, Sirajudeen KN, Salleh MS. Hypoglycemic and antioxidant effects of honey supplementation in streptozotocin-induced diabetic rats. Int J Vitam Nutr Res 2010;80:74-82.

Tan HT, Rahman RA, Gan SH, Halim AS, Hassan SA, Sulaiman SA,et al. The antibacterial properties of Malaysian Tualang honey against wound and enteric microorganisms in comparison to manuka honey. BMC Complement Altern Med 2009;9:34.

Pratibha JS, Manita TW. Antibacterial activity of honey against ESBL producing Klebsiella pneumoniae from burn wound infections. Int J Curr Pharm Res 2015;7:32-6.

Kassim M, Achoui M, Mustafa MR, Mohd MA, Yusoff KM. Ellagic acid, phenolic acids and flavonoids in Malaysian honey extracts demonstrate in vitro anti-inflammatory activity. Nutr Res 2010;30:650 9.

Koc AN, Silici S, Kasap F, Hormet-Oz HT, Mavus-Buldu H, Ercal BD. Antifungal activity of the honeybee products against Candida spp. and Trichosporon spp. J Med Food 2011;14:128-34.

Al-Waili NS, Saloom KY, Al-Waili TN, Al-Waili AN, Akmal M, Al- Waili FS, et al. Influence of various diet regimens on deterioration of hepatic function and hematological parameters following carbon tetrachloride: A potential protective role of natural honey. Nat Prod Res 2006;20:1258-64.

Zaid SS, Sulaiman SA, Sirajudeen KN, Othman NH. The effects of Tualang honey on female reproductive organs, tibia bone and hormonal profile in ovariectomised rats-animal model for menopause. BMC Complement Altern Med 2011;10:82.

Gharzouli K, Amira S, Gharzouli A, Khennouf S. Gastroprotective effects of honey and glucose-fructose-sucrose-maltose mixture against ethanol-, indomethacin- and acidified aspirin-induced lesions in the rat. Exp Toxicol Pathol 2002;54:217-21.

Sushanth KA, Lakshmi KC, Reddy DS. Evaluation of wound healing activity with a new formulation of dry Mangifera indica and honey using swiss albino mice. Asian J Pharm Clin Res 2016;9:139-42.

Arzoo, Parle M. Anti-obsessive-compulsive activity of honey. Asian J Pharm Clin Res 2017;10(5):206-9.

Elbakry KA, Malak CA, Howas MM. Immunomodulatory role of honey and propolis on carbon tetrachloride (CCL4) injected rats. Int J Pharm PharmSci 2015;7:259-62.

Yap P, Bakar MF. Physicochemical, phytochemical and antimicrobial properties of wild honey collected at mangrove and mountain areas in Sabah, Malaysian Borneo. Int J Pharm PharmSci 2014;6:287-92.

Luis G, Rubio C, GutiÃ©rrez AJ, HernÃ¡ndez C, GonzÃ¡lez-Weller D, Revert C, et al. Palm tree syrup; Nutritional composition of a natural edulcorant. Nutr Hosp 2012;27:548-52.

Radam RR, Sari HN, Lusyani H. Chemical compounds of granulated palm sugar made from sap of nipa palm (Nypa fruticans Wurmb) growing in three different places. J Wetlands Environ Manage 2014;2:108-14.

Guerra MJ, Mujica MV. Physical and chemical properties of granulated cane sugar panelasâ€. Cienc Tecnol Aliment Campinas 2010;30:250-7.

Al-Mamary M, Al-Meeri A, Al-Habori M. Antioxidant activities and total phenolics of different types of honey. Nutr Res 2002;22:1041-7.

Al ML, Daniel D, Moise A, Bobis O, Laslo L, Bogdanov S. Physico-chemical and bioactive properties of different floral origin honeys from Romania. Food Chem 2009;112:863-7.

Erejuwa OO, Sulaiman SA, Wahab MS. Honey: A novel antioxidant. Molecules 2012;17:4400-23.

Jantakee K, Tragoolpua Y. Activities of different types of Thai honey on pathogenic bacteria causing skin diseases, tyrosinase enzyme and generating free radicals. Biol Res 2015;48:4.

Bundit T, Anothai T, Pattaramart P, Roongpet T, Chuleeporn S. Comparison of antioxidant contents of Thai honeys to Manuka honey. Mal J Nutr 2016;22:413-20.

Chua LS, Rahaman NL, Adnan NA, Tan TT. Antioxidant activity of three honey samples in relation with their biochemical components. J Anal Methods Chem 2013;2013:313798.

Peerajan S, Chaiyasut C, Sirilun S, Chaiyasut K, Kesika P, Sivamaruthi BS. Enrichment of nutritional value of Phyllanthus emblica fruit juice using the probiotic bacterium, Lactobacillus paracasei HII01 mediated fermentation. Food Sci Technol Campinas 2016;36:116-23.

Chaiyasut C, Kesika P, Chaiyasut K, Sittiyuno P, Peerajan S, Sivamaruthi BS. Total phenolic content and free radical scavenging activity of representative medicinal plants of Thailand. Asian J Pharm Clin Res 2017;10:137-41.

Sivamaruthi BS, Pengkumsri N, Saelee M, Kesika P, Sirilun S, Peerajan S, et al. Impact of physical treatments on stability and radical scavenging capacity of anthocyanidins. Int J Pharm Pharm Sci 2016;8:162-7.

Chaiyasut C, Sivamaruthi BS, Pengkumsri N, Sirilun S, Peerajan S, Chaiyasut K, et al. Anthocyanin profile and its antioxidant activity of widely used fruits, vegetables, and flowers in Thailand. Asian J Pharm Clin Res 2016;9:218-24.

Kucuk M, Kolayh S, Karaoglu S, Ulusoy E, Baltaci C, Candan F. Biological activities and chemical composition of three honeys of different types from Anatolia. Food Chem 2007;100:526-34.

Saxena S, Gautam S, Sharma A. Physical, biochemical and antioxidant properties of some Indian honeys. Food Chem 2010;118:391-7.

Estevinho L, Pereira AP, Moreira L, Dias LG, Pereira E. Antioxidant and antimicrobial effects of phenolic compounds extracts of Northeast Portugal honey. Food Chem Toxicol 2008;46:3774-9.

Suntiparapop K, Prapaipong P, Chantawannakul P. Chemical and biological properties of honey from Thai stingless bee (Tetragonula leaviceps). J Apicult Res 2012;51:45-52.

Low RHP, Baba AS, Aboulfazli F. Effects of different levels of refined cane sugar and unrefined coconut palm sugar on the survivability of Lactobacillus acidophilus in probiotic ice cream and its sensory and antioxidant properties. Food SciTechnol Res 2015;21:857-62.

Phillips KM, Carlsen MH, Blomhoff R. Total antioxidant content of alternatives to refined sugar. J Am Diet Assoc 2009;109:64-71.