THE EFFECT OF PROPOLIS EXTRACT AND PROPOLIS CANDIES ON THE GROWTH OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS ATCC 43718
DOI:
https://doi.org/10.22159/ajpcr.2017.v10s5.23086Keywords:
Propolis, Flavonoid, Aggregatibacter actinomycetemcomitans, PeriodontitisAbstract
Objective: This experiment aimed to analyze the effect of propolis extract and propolis containing candies on the growth of Aggregatibacter actinomycetemcomitans using spectrophotometric analysis and colony-forming units (CFU) counts.
Methods: After A. actinomycetemcomitans were exposed to propolis extract and candies, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined with spectrophotometry and post-exposure colony counting.
Results: The MIC of propolis extract against A. actinomycetemcomitans was determined to be 10%, and the MBC was 20%. A decrease in the total CFU count of A. actinomycetemcomitans was observed after propolis extract and candy exposure.
Conclusions: Propolis extract and propolis candies were effective in inhibiting the growth of A. actinomycetemcomitans ATCC 43718 in vitro.
Downloads
References
Kesic L, Petrovic M, Obradovic R, Pejcic A. The importance of Aggregatibacter actinomycetemcomitans in etiology of periodontal
disease-mini review. Acta Med Med 2009;48(3):35-7.
Agarwal G, Vemanaradhya GG, Mehta DS. Evaluation of chemical composition and efficacy of Chinese propolis extract on Porphyromonas
gingivalis and Aggregatibacter actinomycetemcomitans: An in vitro study. Contemp Clin Dent 2012;3(3):256-61.
Gomes RT, Lemos S, Abreu R, Gerais M, Horizonte B. Periodontitis treatment with Brazilian green propolis gel. Microbiol 2006;3:336-41.
Taylor SL, Lang SD. Aggregatibacter actinomycetemcomitans (Actinobacillus actinomycetemcomitans). Infect Dis Antimicrob.
Available from: http://www.antimicrobe.org/b72.asp.
Kim MJ, Kim CS, Kim BH, Ro SB, Lim YK, Park SN, et al. Antimicrobial effect of Korean propolis against the mutans streptococci
isolated from Korean. J Microbiol 2011;49(1):161-4. Coutinho A. Honeybee propolis extract in periodontal treatment:
A clinical and microbiological study of propolis in periodontal treatment. Indian J Dent Res 2012;23(2):294. Tulsani SG, Chikkanarasaiah N, Siddaiah SB, Krishnamurthy NH. The effect of Propolis and Xylitol chewing gums on salivary Streptococcus mutans count: a clinical trial. Indian J Dent Res 2014;25(6):737-41. Khalil ML. Biological activity of bee propolis in health and disease. Asian Pac J Cancer Prev 2006;7:22-31.
Patel HR, Krishnan CG, Thanveer K. Antimicrobial effect of honey on Streptococcus mutans-an in vitro study. Int J Dent Sci Res
;1(2):46-9. Ahmadi-Motamayel F, Hendi SS, Alikhani MY, Khamverdi Z. Antibacterial activity of honey on cariogenic bacteria. J Dent (Tehran) 2013;10(1):10-5. Bali EB, Açik L, Akca G, Sarper M, Elçi MP, Avcu F, et al. Antimicrobial activity against periodontopathogenic bacteria, antioxidant and cytotoxic effects of various extracts from endemic Thermopsis turcica. Asian Pac J Trop Biomed 2014;4(7):505-14.
Mehdi FM, Abdollah B, Ali FJ, Zahra H, Khosrow Z, Ehsan YM. An in vitro study on the anti-bacterial effect of Ferula assa-foetida L.
And quercus infectoria olivier extracts on Steptococcus mutans and Streptococcus sanguis. Avicenna J Dent Res 2015;7(1):1-5.
Dzoyem JP, Hamamoto H, Ngameni B, Ngadjui BT, Sekimizu K. Antimicrobial action mechanism of flavonoids from Dorstenia species.
Drug Discov Ther 2013;7(2):66-72.
Gebara EC, Lima LA, Mayer MP. Propolis antimicrobial activity against periodontopathic bacteria. Braz J Microbiol 2002;33(4):365-9.
Sutton S. Measurement of microbial cells by optical density. J Validation Technol 2011;17(1):46-9.
Research and Community Service Division. Bogor Agricultural Institute; 2011. Available from: http://www.lppm.ipb.ac.id/index.
php?option=com_content&view=article&id=2063:kenapa-gula-arenlebih-sehat-dari-gula-pasir&catid=38:warta-iptek&Itemid=50.
Mythireyi D, Krishnababa M. Aggregatibacter actinomycetemcomitans, an aggressive oral bacteria-a review. Int J Health Sci Res
;2(5):105-17.
Anonymous. Carbohydrate Metabolism in Bacteria-Use of Differences in Carbohydrate Metabolism for Identifying Bacteria; 2015. Available
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.