EFFECTS OF HUMAN PLATELET LYSATES WITHOUT ADDITIONAL GROWTH FACTORS ON THE PROTEIN PROFILES OF HUMAN UMBILICAL VEIN ENDOTHELIAL CELL CULTURE MEDIA

Hazriani Ra; Lisa Amir; Ratna Farida

doi:10.22159/ajpcr.2017.v10s5.23095

Authors

Hazriani Ra Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Depok, Indonesia
Lisa Amir Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Depok, Indonesia
Ratna Farida Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Depok, Indonesia

DOI:

https://doi.org/10.22159/ajpcr.2017.v10s5.23095

Keywords:

Fetal bovine serum, Growth factor, Human platelet lysate, Human umbilical vein endothelial cell, Protein profile

Abstract

Objectives: The objective of this study is to evaluate the effect of HPLs with no additional GFs on the HUVEC protein profile.

Methods: HUVEC cultures were examined in groups as follows: Fetal bovine serum (FBS), 2%-HPL with a GF, and 2%- and 5%-HPL without a GF which were analyzed with a sodium dodecyl sulfate-polyacrylamide gel electrophoresis test.

Results: The intensity, thickness, and molecular weight of HUVEC band proteins cultured without a GF were not significantly different compared to the control groups (FBS or HPL with a GF).

Conclusions: No difference was found in the HUVEC protein profile after they were cultured with FBS and HPLs, with or without GFs.

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