ALPHA-GLUCOSIDASE INHIBITING ACTIVITY FROM THE MOST ACTIVE FRACTION OF CALOPHYLLUM TOMENTOSUM, WIGHT LEAVES

Berna Elya; Nuraini Puspitasari; Fasya Hadaina Maharani

doi:10.22159/ajpcr.2017.v10s5.23101

Authors

Berna Elya Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
Nuraini Puspitasari Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
Fasya Hadaina Maharani Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10s5.23101

Keywords:

Diabetes mellitus, Calophyllum tomentosum Wight, Fractionation, Nil, Enzyme inhibitory assay

Abstract

Objective: The purpose of this study was to obtain the inhibitory concentration, 50% value from the most active fraction of methanolic extract of leaf Calophyllum tomentosum, Wight, and to identify the group of compounds contained in the most active fraction.

Methods: The methanol extract was fractionated using the silica gel static phase, the n-hexane motion phase, ethyl acetate, and methanol enhanced by its polarity.

Results: There were nine selected fractions tested in inhibitory activity using an enzyme activity inhibitory assay.

Conclusions: The results showed the inhibition by the most active fraction is 51.288 Î¼g/mL; this fraction contains of flavonoid, saponin, and terpenoid.

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