ANTIOXIDANT ACTIVITY AND LIPOXYGENASE INHIBITORY ASSAY WITH TOTAL FLAVONOID CONTENT OF GARCINIA LATERIFLORA BLUME LEAVES EXTRACT

Sismita Avelia; Marista Gilang Mauldina; Berna Elya

doi:10.22159/ajpcr.2017.v10s5.23122

Authors

Sismita Avelia Department of Phytochemistry and Pharmacognosy, Faculty of Pharmacy, Universitas Indonesia, Jakarta, Indonesia.
Marista Gilang Mauldina Department of Phytochemistry and Pharmacognosy, Faculty of Pharmacy, Universitas Indonesia, Jakarta, Indonesia.
Berna Elya Department of Phytochemistry and Pharmacognosy, Faculty of Pharmacy, Universitas Indonesia, Jakarta, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10s5.23122

Keywords:

Antioxidant, Ferric reducing antioxidant power, Garcinia lateriflora Blume, Flavonoid, Lipoxygenase

Abstract

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Objective: Garcinia lateriflora Blume has been reported to have antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl method with methanol, ethyl acetate, and n-hexane leaves extract inhibitory concentration 50% (IC50) levels of 6.18, 8.03, and 156.8 Î¼g/mL, respectively. Meanwhile, there has been no literature regarding G. lateriflora Blume's lipoxygenase inhibition activity. The aim of this study was to determine the potential antioxidant activity and lipoxygenase inhibition activity of three leaf extracts of G. lateriflora Blume.

Methods: These study test methods involved an assessment of antioxidant activity using the ferric reducing antioxidant power method, an assessment of lipoxygenase inhibition activity through the in vitro method, and a qualitative analysis of flavonoid and total flavonoid content using thin-layer chromatography and the AlCl3 colorimetric method to reveal the most active extract.

Results: Based on the assessment for methanol, ethyl acetate, and n-hexane, the results showed that the effective concentration 50% levels of the antioxidant activity of G. lateriflora Blume leaves extract were 9.567, 16.555, and 50.550 Î¼g/mL, respectively. Furthermore, the IC50 levels of the lipoxygenase inhibition activity were 0.693, 0.793, and 1.316 Î¼g/mL, respectively. The most active extract for both of the tests was methanol extract, which has a total flavonoid content of 6.298 mg quercetin equivalents/g.

Conclusions: Based on the test results, it can be concluded that G. lateriflora Blume leaves extracts exhibit antioxidant and lipoxygenase inhibition activities, with methanol extract as the most active extract, containing more flavonoid than the other two extracts.

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References

Nichols JA, Katiyar SK. Skin photoprotection by natural polyphenols: Anti-inflammatory, antioxidant and DNA repair mechanisms. Arch Dermatol Res 2010;302(2):71-83.

Ritthiwigrom T, Laphookhieo S, Pyne SG. Chemical constituents and biological activities of Garcinia cowa Roxb. Maejo Int J Sci Technol 2013;7(2):212-31.

Katrin EB, Munâ€™im A, Hasiholan A, Marlin I, Mailandari M. Antioxidant activities of leaves extracts of three species of Garcinia. Int J Med Aromat Plants 2012;2(4):691-3.

Seruji NM, Khong HY, Kutoi CJ. Antioxidant, anti-inflammatory, and cytotoxic activities of Garcinia nervosa (Clusiaceae) J Chem 2012;2013(913406):1-6.

Padhye S, Ahmad A, Oswal N, Sarkar FH. Emerging role of garcinol, the antioxidant chalcone from Garcinia indica Choisy and its synthetic analogs. J Hematol Oncol 2009;2:38.

Deschamps JD, Kenyon VA, Holman TR. Baicalein is a potent in vitro inhibitor against both reticulocyte 15-human and platelet 12-human lipoxygenases. Bioorg Med Chem 2006;14(12):4295-301.

Wong C, Cheung W, Lau Y, De la TA, Owusu-Apenten R. Food and nutrition report a FRAP assay at pH 7 unveils extra antioxidant activity from green, black, white and rooibos tea but not apple tea. Food Nutr Rep 2015;1(1):16-23.

Fidrianny I, Aristya T, Hartati R. Antioxidant capacities of various leaves extracts from three species of legumes and correlation with total flavonoid, phenolic, carotenoid content. Int J Pharmacogn Phytochem Res 2015;7(3):628-34.

Chang H, Yang L. Radical-scavenging and rat liver mitochondria lipid peroxidative inhibitory effects of natural flavonoids from traditional medicinal herbs. J Med Plants Res 2012;6(6):997-1006.

Abdullah AR, Bakhari NA, Osman H. Study on the relationship of the phenolic, flavonoid and tannin content to the antioxidant activity of Garcinia atroviridis. Univ J Appl Sci 2013;1(3):95-100.

Santosa D, Haresmita PP. Antioxidant activity determination Garcinia dulcis (Roxb.) Kurz, Blumeamollis (D. Don) Merr., Siegesbeckia orientalis L., and Salvia riparia H. B. K which collected from Taman nasional Gunung Merapi using DPPH (2,2-diphenyl-1-pikril-hidrazil). Tradit Med J 2015;20(1):28-36.