ANTI-INFLAMMATORY ACTIVITY OF TINOCRISPOSIDE BY INHIBITING NITRIC OXIDE PRODUCTION IN LIPOPOLYSACCHARIDES-STIMULATED RAW 264.7 CELLS

Adek Zamrud Adnan; Muhammad Taher; Tika Afriani; Annisa Fauzana; Dewi Imelda Roesma; Andani Eka Putra

doi:10.22159/ajpcr.2018.v11i4.23739

Authors

Adek Zamrud Adnan Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Andalas University, Padang, Indonesia.
Muhammad Taher Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia, 25200 Kuantan, Malaysia.
Tika Afriani Department of Pharmacy, Mohammad Natsir University, Bukittinggi 26136, Indonesia.
Annisa Fauzana Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Andalas University, Padang, Indonesia.
Dewi Imelda Roesma Department of Biology, Andalas University, Indonesia.
Andani Eka Putra Department of Microbiology, Medicinal Faculty, Andalas, University, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i4.23739

Keywords:

Anti-inflammatory activity, Griess method, Tinocrisposide, Tinospora crispa

Abstract

Â Objective: The aim of this study was to investigate in vitro anti-inflammatory activity of tinocrisposide using lipopolysaccharides (LPS)-stimulated RAW 264.7 macrophage cells. Tinocrisposide is a furano diterpene glycoside that was isolated in our previous study from Tinospora crispa.

Methods: Anti-inflammatory effect was quantified spectrometrically using Griess method by measuring nitric oxide (NO) production after the addition of Griess reagent.

Results: The sample concentrations of 1, 5, 25, 50, and 100 Î¼M and 100 Î¼M of dexamethasone (positive control) have been tested against the LPS-stimulated RAW 264.7 cells, and the results showed NO level production of 39.23, 34.00, 28.9, 20.25, 16.3, and 13.68 Î¼M, respectively, and the inhibition level of 22.67, 33.00, 43.03, 60.10, 68.00, and 73%, respectively.

Conclusions: From the study, it could be concluded that tinocrisposide was able to inhibit the formation of NO in the LPS-stimulated RAW 264.7 cells in concentration activity-dependent manner, with half-maximal inhibition concentration 46.92 Î¼M. It can be developed as anti-inflammatory candidate drug because NO is a reactive nitrogen species which is produced by NO synthase. The production of NO has been established as a mediator in inflammatory diseases.

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Author Biography

Adek Zamrud Adnan, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Andalas University, Padang, Indonesia.

Professor of Faculty of Pharmacy of Andalas University Padang Indonesia

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