ANTIHYPERGLYCEMIC ACTIVITY OF TINOCRISPOSIDE BY STIMULATING 3T3-L1 ADIPOCYTE CELL DIFFERENTIATION

Adek Zamrud Adnan; Muhammad Taher; Annisa Fauzana; Tika Afriani; Dewi Imelda Roesma; Andani Eka Putra

doi:10.22159/ajpcr.2018.v11i11.29304

Authors

Adek Zamrud Adnan Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Andalas University, Padang, Indonesia.
Muhammad Taher Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia, 25200 Kuantan, Malaysia.
Annisa Fauzana Department of Pharmaceutical and Food Analyses, Universitas Abdurrab, Pekanbaru, 28293, Indonesia.
Tika Afriani Department of Pharmacy, Mohammad Natsir University, Bukittinggi 26136, Indonesia.
Dewi Imelda Roesma Department of Biology, Andalas University, Indonesia.
Andani Eka Putra Department of Microbiology, Medicinal Faculty, Andalas, University, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i11.29304

Keywords:

3T3-L1 adipocyte cell differentiation, Antihyperglycemic, Tinocrisposide, Tinospora crispa

Abstract

Objective: The aim of this study is to investigate the antihyperglycemic activity of tinocrisposide by stimulating 3T3-L1 adipocyte cell differentiation. Tinocrisposide is a furanoditerpene glycoside that was isolated from Tinospora crispa (Menispermaceae).

Methods: Adipocyte cell differentiation activity of tinocrisposide in interval concentrations of 50, 25, 12.5, and 6.25 Î¼g/ml has been investigated on 3T3-L1 cell line using insulin of 1 Î¼g/ml as a positive and Dulbecco's modified Eagle media (DMEM) as a negative control group. The effect of tinocrisposide was quantified with oil red O staining method by measuring an absorbance of lipid solution in isopropanol at a wavelength (Î») of 520 nm.

Results: Tinocrisposide in the concentrations of 50, 25, 12.5, and 6.25 Î¼g/ml insulin of 1 Î¼g/ml and DMEM groups showed absorbance value of 0.7669, 0.7253, 0.6563, 0.6481, 0.954, and 0.2653, respectively. It was found that there was a significant difference statistically in lipid droplets accumulation among all groups (p<0.05) and tinocrisposide at a concentration of 50 Î¼g/ml showed the highest lipid droplets accumulation in 3T3-L1 adipocyte cells.

Conclusion: From the study, it could be concluded that tinocrisposide was able to stimulate the differentiation of adipocyte cell and had antihyperglycemic activity.

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Author Biography

Adek Zamrud Adnan, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Andalas University, Padang, Indonesia.

Professor of Faculty of Pharmacy of Andalas University Padang Indonesia

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