DEVELOPMENT AND STABILITY EVALUATION OF VAGINAL SUPPOSITORY CONTAINING GLYCYRRHIZA GLABRA L. FOR THE TREATMENT OF CANDIDA ALBICANS INFECTION

Sasithorn Sirilun; Bhagavathi Sundaram Sivamaruthi; Periyanaina Kesika; Noppawat Pengkumsri; Nucharee Tuntisuwanno; Khontaros Chaiyasut; Sartjin Peerajan; Chaiyavat CHAIYASUT

doi:10.22159/ajpcr.2018.v11i7.25927

Authors

Sasithorn Sirilun Innovation Center for Holistic Health, Nutraceuticals and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
Bhagavathi Sundaram Sivamaruthi Innovation Center for Holistic Health, Nutraceuticals and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
Periyanaina Kesika Innovation Center for Holistic Health, Nutraceuticals and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
Noppawat Pengkumsri Innovation Center for Holistic Health, Nutraceuticals and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
Nucharee Tuntisuwanno Innovation Center for Holistic Health, Nutraceuticals and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
Khontaros Chaiyasut Health Innovation Institute, Chiang Mai 50200, Thailand.
Sartjin Peerajan Health Innovation Institute, Chiang Mai 50200, Thailand.
Chaiyavat CHAIYASUT Innovation Center for Holistic Health, Nutraceuticals and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i7.25927

Keywords:

Antimicrobial activity, Candida albicans, Glycyrrhiza glabra L, Suppository formula, Licorice, Ethanolic extract

Abstract

Objective: The present study assessed the antioxidant activity, phytochemical content, and anti-Candida albicans property of ethanolic extract of Glycyrrhiza glabra L. (licorice). In addition, suppository formula (SF) was developed with licorice extract (LE), and the stability of SF was evaluated.

Methods: The total phenolic and flavonoid content was measured by colorimetric methods. 2, 2â€²-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS), ferric reducing antioxidant power (FRAP), inhibition of lipid peroxidation (LPO), nitric oxide (NO), and superoxide (SO) radical scavenging assays were performed to evaluate antioxidant property. Antimicrobial activity was determined by plating method. The active principle was determined by high-performance liquid chromatography method.

Results: The licorice sample was extracted with 95% ethanol, and 26.91Â±1.35% of yield was observed. The LE contains phenolic acids (167.70Â±3.18 mg gallic acid equivalent/g extract), flavonoids (162.53Â±9.95 mg quercetin equivalent/g extract), and glabridin (3.90Â±0.05 mg/g extract). LE exhibited the scavenging activity in terms of 810.53Â±25.37 Î¼M of Trolox equivalent/mg of extract, 165.04Â±5.10 Î¼M of FeSO4 equivalent/mg of extract, and 3750.35Â±1.25, 68.25Â±0.07, and 511Â±0.80 Î¼M of Trolox equivalent/mg of extract in ABTS, FRAP, LPO, NO, and SO assays, respectively. About â‰¥62.50, â‰¥125, and â‰¥250 Î¼g/mL of LE was recorded as minimal inhibitory concentration against C. albicans, Lactobacillus casei, and Lactobacillus acidophilus, respectively. The SF was formulated and the stability was evaluated. The activity and color of SFs did not differ on storage. Moreover, no spot formation was observed.

Conclusion: The SF with LE could be the safe therapeutic strategy for the treatment of candida infection in vaginal region.

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