IN VITRO ANTIBACTERIAL ACTIVITY OF THE ETHANOLIC EXTRACT OF JALOH (SALIX TETRASPERMA ROXB.) LEAVES AGAINST STAPHYLOCOCCUS AUREUS AND PSEUDOMONAS AERUGINOSA
DOI:
https://doi.org/10.22159/ajpcr.2018.v11s1.26580Keywords:
Antibacterial, Salix tetrasperma Roxb, Jaloh, Staphylococcus aureus, Pseudomonas aeruginosaAbstract
Objective: This study aims to determine antibacterial activity of ethanolic extract of jaloh (Salix tetrasperma Roxb.) leaves against Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA).
Methods: Extract was obtained by maceration method of jaloh (S. tetrasperma Roxb.) leaves dried powder with 96% ethanol as solvent. The antibacterial activities of extract were tested by Kirby–Bauer method against SA and PA. Data were analyzed statistically using Kruskal–Wallis test for significant difference level p<0.05.
Results: Based on the regression test, the equation of regression curve of extract antibacterial activity on SA and PA, respectively, was y=350.456x-229.579 and y=331.866x-272.069. The minimum inhibitory concentrations (MICs) of SA and PA from the equation of regression curve, respectively, were 4.5193 and 6.6039 mg/mL.
Conclusion: Based on the MIC value, ethanolic jaloh leaves extract had a weak antibacterial activity against SA and PA.
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