ANALYSIS OF BIOLOGICAL ACTIVITY AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY STUDY OF CONVENTIONAL EXTRACTION OF VITEX NEGUNDO LINN. LEAVES

Authors

  • Samreen Fatema Department of Chemistry, Maulana Azad College, Aurangabad, Maharashtra, India.
  • Milind Ubale Department of Chemistry, Vasantrao Naik College, Aurangabad, Maharashtra, India.
  • Mazahar Farooqui Department of Chemistry, Dr. Rafiq Zakaria College for Women, Navkhanda, Aurangabad, Maharashtra, India.
  • Pathan Mohd Arif Department of Chemistry, Maulana Azad College, Aurangabad, Maharashtra, India. http://orcid.org/0000-0001-8402-3450

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i1.27854

Keywords:

Vitex negundo, Antioxidant activity, Gas chromatography-mass spectrometry study, Aqueous extraction

Abstract

Objective: The main objective of the present work is to carry out the biological activity, gas chromatography-mass spectrometry (GC-MS) studies for the possible compounds present in Vitex negundo.

Methods: The aqueous extract of V. negundo Linn. was screened for biological activities such as antimicrobial, antituberculosis (TB), antimalarial, and antioxidant activities. The GC-MS analysis was carried out.

Results: The result shows that leaf extract is effective against Escherichia Coli and Bacillus subtitus while negative results for anti-TB and anti-malarial activity. The antioxidant activity of the leave extract is excellent.

Conclusion: The compounds present in the leaf extract of V. negundo are responsible for possessing the biological activity.

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Author Biographies

Samreen Fatema, Department of Chemistry, Maulana Azad College, Aurangabad, Maharashtra, India.

department of chemistry

Pathan Mohd Arif, Department of Chemistry, Maulana Azad College, Aurangabad, Maharashtra, India.

ASSOCIATE PROFESSOR, AND

INCHARGE, POST GRADUATE AND RESEARCH CENTRE, Maulana Azad College, Aurangabad (MS), India 431001

References

Murthy JR, Venkataraman S, MeeraKiran R, Deshmukh S, Chidambaraathan N, Devi P. Phytochemical investigation and antipyretic activity of leaf extract of Vitex negundo linn. Int J Pharmtech Res 2010;2:1068-73.

Mohanraj S, Vanathi P, Barniy NS, Saravaman D. Antimicrobial effectiveness of Vitex negundo leaf extracts. Indian J Fiber Text Res 2012;37:389-92.

Kannathasan K, Senthilkumar A, Venkatesalu V, Chandrasekaran M. Larvicidal activity of fatty acid methyl esters of Vitex species against Culex quinquefasciatus. Parasitol Res 2008;103:999-1001.

Karunamoorthi K, Ramanujam S, Rathinasamy R. Evaluation of leaf extracts of Vitex negundo L. (Family: Verbenaceae) against larvae of Culex tritaeniorhynchus and repellent activity on adult vector mosquitoes. Parasitol Res 2008;103:545-50.

Vadawate AV, Barve DM, Dave AM. In vitro flowering and rapid propagation of Vitex negundo L. – A medicinal plant. Indian J Biotechnol 2006;5:112-6.

Jeyaseelan EC, Pathmanathan MK, Jeyadeven JP. Inhibitory effect of different solvent extracts of Vitex negundo L. and Allium L. on phytopathogenic bacteria. Scholar Res Libr 2011;3:1-8.

Aswar PB, Khadabadi SS, Kuchekar BS, Rajukar RM, Saboo SS, Javarkar RD. In vitro evaluation of antibacterial and anti-fungal activity of Vitex negundo (Verbenacceae). Technobotanical Leaf 2009;13:962-7.

Talekar YP, Das B, Paul T, Talekar DY, Apte KG. Wound healing potential of Vitex negundo linn in experimental animals. Int J Pharm Pharm Sci 2012;4: 543-6.

Roosewelt C, Vincent S, Sujith K, Darwin CR. Wound healing activity of methanolic extract of Vitex negundo leaves in albino wistar rats. J Pharm Res 2011;4:2553-5.

Sharma A, Tyagi S, Nag R, Chaturvedi A, Nag TN. Antimicrobial activity and cellular toxicity of flavonoids extracts from Pongamia pinnata and Vitex negundo. Rom Biotechnol Lett 2011;16:6397-400.

Chowdhury FB, Azam FM, Hassan M, Jahan FI, Choedhury AR, Seraj S, et al. Studies with callus induction of Vitex negundo: As an aromatic medicinal plant. Am Eurasian J Sustain Agric 2011;5:6-14.

Adnaik RS, Pai PT, Mule SN, Naikwade NS, Magdum CS. Laxative activity of Vitex negundo linn leaves. Asian J Exp Sci 2008;22:159-60.

Dewade DR, Christina AJ, Chidambaranathan N, Bhaipale NS, Tekade NP. Antitumor activity of Vitex negundo linnagainst Dalton’s Ascitic Lymphoma. Int J Phaemtech Res 2010;2:1101-4.

Thombre R, Jagtap R, Patil N. Evaluation of phytoconstituents, antibacterial, antioxidant and cytotoxic activity of Vitex negundo l and Tabernaemontana divaricata l. Int J Pharm Biosci 2013;4:389-96.

Fatema S, Farooqui M, Permila G, Arif PM. The phytochemical and antioxidant property of ethereal extract of Hibiscus rosa-sinensis leaves extract. Res J Pharm Biol Chem Sci 2018;9:603-8.

Altameme HJ, Hameed IH, Abu-Serag NA. Analysis of bioactive phytochemical compounds of two medicinal plants, Equisetum arvense and Alchemila valgaris seeds using gas chromatography-mass spectrometry and fourier–transform infrared spectroscopy. Malays Appl Boil 2015;44:47-58.

Barreira JC, Ferreeira IC, Oliveira BP, Pereira JA. Antioxidant activities of the extracts from chestnut flower, leaf, skins and fruit. Food Chem 2008;107:1106-13.

Hua-Bin L, Ka-Wing C, Chi-Chu W, King-Wai F, Chen F, Jiang Y. Evaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgae. Food Chem 2007;102:771-6.

Kardong D, Upadhyaya S, Saikia LR. Screening of phytochemicals, antioxidant and antibacterial activity of crude extract of Pteridium aquilinum kuhn. J Pharm Res 2013;6:179-82.

Gupta R, Singh RL, Dwivedi N. In vitro antioxidant activity and GC-MS analysis of the ethanolic extract of Terminelia bellirica roxb (Baheda). Int J Pharm Pharm Sci 2016;8:275-82.

Amala VE, Jeyraj M. Determination of antibacterial, antifungal, bioactive constituents of triphala by FT-IR and GC-MS analysis. Int J Pharm Pharm Sci 2014;6:123-6.

Praveen Kumar P, Kumaravel S, Lalitha C. Screening of antioxidant activity, total phenolics and GC-MS study of Vitex negundo. Afr J Biochem Res 2010;4:191-5.

Hema R, Kumaravel S, Alagundaram K. GC/MS determination of bioactive components of Murraya Koenigii. J Am Sci 2011;7:80-3.

Akpuaka A, Ekwenchi MM, Dashak DA, Dildar A. Biological activities of characterized isolated of n-hexane extract of Azadirachta indica a juss (Neem) leaves. Nat Sci 2013;11:119-24.

Kalaivani CS, Sathish SS, Jankiraman N, Johnson M. GC-MS studies on Andrographis paniculata (burm. F.) wall ex. Nees - A medicinally important plant. Int J Med Aromat Plants 2012;2:69-74.

Published

07-01-2019

How to Cite

Fatema, S., M. Ubale, M. Farooqui, and P. M. Arif. “ANALYSIS OF BIOLOGICAL ACTIVITY AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY STUDY OF CONVENTIONAL EXTRACTION OF VITEX NEGUNDO LINN. LEAVES”. Asian Journal of Pharmaceutical and Clinical Research, vol. 12, no. 1, Jan. 2019, pp. 289-92, doi:10.22159/ajpcr.2019.v12i1.27854.

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