QUANTITATIVE ESTIMATION OF BERBERINE CONTENT OF BERBERIS ASIATICA FROM DIFFERENT ALTITUDE OF GARHWAL HIMALAYA

Authors

  • Alok Maithani
  • Versha Parcha
  • Deepak Kumar

Abstract

Objective: Berberis asiatica is a popular medicinal plant of Garhwal Himalaya and its root is traditionally used for curing various ailments like jaundice, conjunctivitis, and diabetes mellitus. In the present study berberine content in B asiatica growing at seven different altitudes of Garhwal Himalayas was quantitatively determined by HPTLC analysis. Methods: Ethanolic extracts of various plant samples were analyzed with standard berberine (purified) in different concentrations 1-5µl. The peak areas of each standard and test samples were obtained from the system and a calibration graph was plotted between concentrations vs. Peak area. Results: The plant samples belonging to lowest altitude region was found to possess maximum concentration of berberine which was 2.94% however berberine content was non-linearly distributed among the other samples of higher altitudes.

Key words:  Berberis asiatica, Berberine, quantitative determination, isoquinoline alkaloid, and HPTLC.

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Published

01-02-2014

How to Cite

Maithani, A., V. Parcha, and D. Kumar. “QUANTITATIVE ESTIMATION OF BERBERINE CONTENT OF BERBERIS ASIATICA FROM DIFFERENT ALTITUDE OF GARHWAL HIMALAYA”. Asian Journal of Pharmaceutical and Clinical Research, vol. 7, no. 6, Feb. 2014, pp. 165-7, https://mail.innovareacademics.in/journals/index.php/ajpcr/article/view/872.