ANTIOXIDANT ACTIVITIES OF FRACTIONS FROM ETHYL ACETATE EXTRACTS OF GARCINIA FRUTICOSA LAUTERB. LEAVES
DOI:
https://doi.org/10.22159/ijap.2018.v10s1.10Keywords:
Antioxidant, 2, 2-Diphenyl-1-picrylhydrazyl, Fractionation, Ferric-reducing antioxidant power, Garcinia fruticosaAbstract
Objective: This study aimed to fractionate the antioxidant activity of the ethyl acetate leaf extract and to characterize the most active fractions
according to compound groups.
Methods: The ethyl acetate extract was fractionated with column chromatography using a gradient elution system. Fractions were first screened
qualitatively for antioxidant activity before active fractions were quantified with respect to in vitro antioxidant activity using the 2,2-diphenyl-1-
picrylhydrazyl (DPPH) radical scavenging assay and the ferric-reducing antioxidant power (FRAP) assay. The compound groups were identified
following separation by thin-layer chromatography.
Results: Fraction 11 exhibited the greatest DPPH radical-scavenging activity, with an IC50 value of 6.58 μg/mL, while the fraction with the greatest
antioxidant activity according to the FRAP assay was fraction 10, with a ferric ion equivalent antioxidant activity value of 1015.34 μmol/g.
Conclusion: Compound group identification revealed that Fractions 10 and 11 contained flavonoids, with two common to both fractions, while
fraction 10 also contained one specific flavonoid.
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