BORON-SILICA BASED MESOPOROUS FOR CURCUMINOID ISOLATION FROM TURMERIC EXTRACT

ADANG FIRMANSYAH; ILMA NUGRAHANI; KOMAR RUSLAN WIRASUTISNA; SLAMET IBRAHIM

doi:10.22159/ijap.2020v12i5.37041

Authors

ADANG FIRMANSYAH School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia, Sekolah Tinggi Farmasi Indonesia, Bandung, Indonesia
ILMA NUGRAHANI School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia
KOMAR RUSLAN WIRASUTISNA School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia
SLAMET IBRAHIM School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia

DOI:

https://doi.org/10.22159/ijap.2020v12i5.37041

Keywords:

Boron-silica based mesoporous, Curcuminoid, Isolation, Turmeric extract

Abstract

Objective: The purpose of this study was to develop the isolation method for curcuminoid from turmeric extract using boron-silica based mesoporous as an adsorbent.

Methods: The formation of mesoporous materials were conducted using the sol-gel technique. The characterization of mesoporous materials was analyzed using a scanning electron microscope (SEM), transmission electron microscope (TEM), and Fourier transforms infrared spectrometry (FTIR). The extraction of turmeric was done by solvent extraction using ethanol 96 %. The isolation of curcuminoid was achieved by the adsorption method using mesoporous materials, both for silica-based mesoporous (MCM) and boron-silica based mesoporous (BMCM). The elution of curcuminoid-loaded mesoporous was conducted using various solvents. The concentration of total curcuminoid and its compounds was measured by visible spectrometry and high-performance liquid chromatography (HPLC).

Results: Morphology of MCM and BMCM shows the homogenous regular spherical shape, but having a different size. MCM has a smaller diameter particle size (500-600 nm) compared to BMCM (700-900 nm). On the other hand, BMCM has a smaller pore size (1-5 nm) compared to MCM (5-20 nm). The FTIR spectra of BMCM shows the additional vibration at 1400-1600 cm for B-O-H bond. Visible spectrometry measurement shows that the highest concentration of curcuminoid eluted from BMCM is 65.411±0.056 ppm by using ethyl acetate as a solvent, while the concentration of curcuminoid eluted from MCM is 11.503±0.054 ppm by using the same solvent. The results of curcuminoid adsorption and elution, indicating that ethyl acetate is the best solvent to elute curcuminoid due to its 98.83 % purity using HPLC analysis.

Conclusion: It was concluded that boron-silica based mesoporous showed stronger curcuminoid adsorption than silica-based mesoporous therefore found to be a potential adsorbent for curcuminoid isolation from turmeric extract.

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