DETERMINATION OF THE ANTIOXIDANT ACTIVITY OF PRASMAN LEAF EXTRACTS (AYAPANA TRIPLINERVIS [VAHL]) AND THE TOTAL FLAVONOID AND PHENOL CONTENTS OF THE MOST ACTIVE EXTRACTS
DOI:
https://doi.org/10.22159/ijap.2020.v12s1.FF021Keywords:
Antioxidants, Ayapana triplinervis (Vahl), 2,2-diphenyl-1-picrylhydrazyl, Ferric reducing antioxidant power, Determination of phenol, Determination of flavonoidAbstract
Objective: Prasman (Ayapana triplinervis [Vahl]) is a plant that can eliminate free radicals through its antioxidant effects. However, little research has
been conducted to explore the antioxidant activity of the plant.
Methods: A. triplinervis (Vahl) leaves, which were determined by the Indonesian Institute of Sciences (LIPI) Bogor, were used in this study. Meanwhile,
extraction was performed using n-hexane, ethyl acetate, and methanol as the solvents. Based on the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay using
a UV–visible spectrophotometer and based on ferric reducing antioxidant power (FRAP) assay using a microplate reader.
Results: Based on the DPPH assay using a UV–visible spectrophotometer, n-hexane, ethyl acetate, and methanol extracts with a final concentration
of 25 μg/mL inhibited DPPH radical production by 38.91, 51.03, and 54.06%, respectively. Using the percent inhibition, the IC50 for the ethyl acetate
and methanol extracts were 28.71 and 23.472 μg/mL, respectively. Based on FRAP assay using a microplate reader, the n-hexane, ethyl acetate, and
methanol extracts had ferrous equivalent antioxidant capacity values of 460, 828.99, and 940.22 μmol/g, respectively. The methanol extract had the
greatest antioxidant activity. The ethyl acetate and methanol extracts at the initial concentrations contained total phenol levels of 12.06 and 42.11 mg
GAE/g extract, respectively, as well as total flavonoid levels of 3.24 and 3.41 mg QE/g extract, respectively.
Conclusion: Based on the determination of phenol and flavonoid levels, the methanol extract had the greatest antioxidant effects.
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References
polyphenolic assay. Int J Food Sci Nutr Diet 2014;3:147-56.
2. Ahmad AR, Juwita R, Siti AD, Malik A. Penetapan kadar fenolik dan
flavonoida total ekstrak metanol buah dan daun patikala (Etlingera
elatior Jack). Pharm Sci Res 2015;2:1-10.
3. Apak R, Gorinstein S, Böhm V, Schaich KM, Özyürek M, Güçlü K.
Methods of measurement and evaluation of natural antioxidant capacity/
activity (IUPAC technical report). Pure Appl Chem 2013;85:957-98.
4. Azizah D, Kumulowati E, Faramayuda F. Penetapan kadar flavonoid
metode AlCl3 pada ekstrak metanol kulit buah kakao (Theobroma
cacao L.). Kartika J Ilmiah Farm 2014;2:45-9.
5. Health Research and Development Agency. Inventory of Indonesian
Medicinal Plants. Jakarta: Department of Health, Republic of
Indonesia; 1991.
6. Badarinath AV, Rao KM, Chetty CM, Ramkanth S, Rajan TV,
Gnanaprakash K. A review on in vitro antioxidant method comparisions,
correlations and considerations. Int J Pharm Tech Res 2010;2:1276-85.
7. Bai MS, Wang C, Zong SC, Lei M, Gao JM. Antioxidant polyketide
phenolic metabolites from the edible mushroom Cortinarius
purpurascens. Food Chem 2013;141:3424-7.
8. Bhaigyabati T, Devi P, Bag G. Total flavonoid content and antioxidant
activity of aqueous rhizome extract of three Hedychium species of
Manipur Valley. Res J Pharm Biol Chem Sci 2014;5:154-9.
9. Blois MS. Antioxidant determinations by the use of a stable free radical.
Nature 1958;181:1199-200.
10. Bolanos de la Torre AA, Henderson T, Nigam PS, Owusu-
Apenten RK. A universally calibrated microplate ferric reducing
antioxidant power (FRAP) assay for foods and applications to Manuka
honey. Food Chem 2015;174:119-23.
11. Bose P, Gupta M, Mazumder UK, Kumar RS, Sivakumar T, Kumar RS.
Hepato protective and antioxidant effects of Eupatorium ayapana
against carbon tetrachloride induced hepatotoxicity in rats. Iran J Pharm
Ther 2007;6:27-33.
12. Closa D, Folch-Puy E. Critical review: Oxygen free radicals and the
systemic inflammatory response systemic inflammatory response. Int
Union Biochem Mol Biol Life 2004;56:185-19.
13. Dalimartha S. Atlas Indonesian Ungaran Medicinal Herbs. Jakarta:
Trubus Agriwidya; 1999.
14. Department of Health, Republic of Indonesia. Common Standard
Parameters of Medicinal Plant Extract. Jakarta: Department of Health,
Republic of Indonesia; 2000. p. 1-12.
15. Gauvin-Bialecki A, Marodon C. Essential oil of Ayapana triplinervis
from Reunion Island: A good natural source of thymohydroquinone
dimethyl ether. Biochem Syst Ecol 2008;36:853-8.
16. Gupta M, Mazumder UK, Chaudhuri I, Chaudhuri RK, Bose P,
Bhattacharya S, et al. Antimicrobial activity of Eupatorium ayapana.
Fitoterapia 2002;73:168-70.
17. Handajani A, Roosihermiatie B, Maryani H. Factors related to mortality
patterns in degenerative diseases in Indonesia. Bul Penelitian Sist
Kesehatan 2010;13:42-53.
18. Harborne JB. Phytochemical Methods. 2nd ed. Kosasih Padmawinata.
Bandung: Penerbit ITB; 1996.
19. Kalay S. Test the Antipyretic Effect of Ethanol Extracts on Prenatal
Leaves (Eupatorium triplinerve Vahl.) in Male Wistar Rats (Rattus
norvegicus L.) which is induced by DTP HB Vaccine. Manado: Faculty
of Mathematics and Sciences, Universitas Sam Ratulangi; 2014.
20. Kikuzaki H, Hisamoto M, Hirose K, Akiyama K, Taniguchi H.
Antioxidant properties of ferulic acid and its related compounds. J Agric
Food Chem 2002;50:2161-8.
21. Kokate CK, Rao RE, Varma KC. Pharmacological studies on the
essential oil of Eupatorium triplinerve Vahl. I. The effects on the central
nervous system and antimicrobial activity. Flavour Ind 1971;2:177-80.
22. Mega IM, Swastini DA. Phytochemical screening and antiradical
activity free of methanol extract of agarwood leaves (Gyrinops
versteegii). J Kim 2010;4:187-92.
23. Molyneux P. The use of the stable free radical diphenylpicrylhidrazyl
(DPPH) for estimating antioxidant activity. Songklanakarin J Sci
Technol 2004;26:211-9.
24. Munte L, Max RR, Citranigtyas G. The antioxidant activity of the
prasman leaf extract. Pharm J Ilmiah Farm 2015;4:41-50.
25. Ozcelik B, Lee J, Min D. Effects of light, oxygen, and pH on
the absorbance of 2, 2-Diphenyl-1-picrylhydrazyl. J Food Sci
2003;68:487-90.
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